Strain Fitness in Pseudomonas putida KT2440 around PP_0965

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,104,505	+				+0.0
1,104,506	-				-3.0
1,104,506	-				-1.6
1,104,583	-				-2.2
1,104,628	+				+0.8
1,104,732	+		PP_0965	0.21	-1.3
1,104,732	+		PP_0965	0.21	-1.4
1,104,732	+		PP_0965	0.21	-0.2
1,104,732	+		PP_0965	0.21	-2.3
1,104,732	+		PP_0965	0.21	-1.8
1,104,733	-		PP_0965	0.21	-4.0
1,104,770	+		PP_0965	0.27	-2.9
1,104,770	+		PP_0965	0.27	-2.6
1,104,771	-		PP_0965	0.27	-1.6
1,104,771	-		PP_0965	0.27	-1.8
1,104,771	-		PP_0965	0.27	-2.1
1,104,771	-		PP_0965	0.27	-1.9
1,104,771	-		PP_0965	0.27	-4.1
1,104,827	+		PP_0965	0.36	-2.1
1,104,845	+		PP_0965	0.39	-2.5
1,104,846	-		PP_0965	0.39	-3.2
1,104,846	-		PP_0965	0.39	-3.6
1,104,898	-		PP_0965	0.47	-0.4
1,104,954	+		PP_0965	0.56	-1.9
1,104,955	-		PP_0965	0.56	-2.6
1,104,955	-		PP_0965	0.56	-1.2
1,104,966	-		PP_0965	0.58	-3.1
1,104,966	-		PP_0965	0.58	+0.1
1,104,966	-		PP_0965	0.58	-4.6
1,104,968	+		PP_0965	0.58	-2.5
1,104,968	+		PP_0965	0.58	-1.4
1,104,969	-		PP_0965	0.58	-1.8
1,105,008	-		PP_0965	0.64	-3.2
1,105,269	-				+0.1
1,105,279	+				-0.5
1,105,280	-				+0.6
1,105,317	-				-1.1
1,105,326	+				-0.6
1,105,326	+				+2.4
1,105,422	-				+0.0
1,105,441	+				+0.3
1,105,441	+				+0.7
1,105,441	+				+1.7
1,105,441	+				-0.0
1,105,442	-				-0.6
1,105,442	-				-2.5
1,105,442	-				-2.6
1,105,481	-				-3.0
1,105,503	+				+1.2
1,105,503	+				-0.3
1,105,503	+				-0.4
1,105,504	-				-1.1
1,105,504	-				+1.4
1,105,504	-				-0.4
1,105,504	-				-3.2
1,105,506	+				-0.5
1,105,507	-				-1.1
1,105,507	-				-2.9
1,105,507	-				-2.5
1,105,776	-		PP_0966	0.19	-2.1
1,105,778	+		PP_0966	0.19	-1.7
1,105,863	+		PP_0966	0.26	-1.3
1,105,926	+		PP_0966	0.31	-3.3
1,105,927	-		PP_0966	0.31	-2.4
1,105,927	-		PP_0966	0.31	-1.2
1,105,927	-		PP_0966	0.31	-2.9
1,105,927	-		PP_0966	0.31	+0.4
1,105,951	-		PP_0966	0.32	-2.8
1,106,184	+		PP_0966	0.50	-3.0
1,106,184	+		PP_0966	0.50	-3.4
1,106,184	+		PP_0966	0.50	-0.6
1,106,184	+		PP_0966	0.50	-0.4
1,106,184	+		PP_0966	0.50	-3.0
1,106,185	-		PP_0966	0.50	-1.3
1,106,185	-		PP_0966	0.50	-1.3
1,106,185	-		PP_0966	0.50	-2.5
1,106,185	-		PP_0966	0.50	-2.1
1,106,185	-		PP_0966	0.50	-3.7
1,106,185	-		PP_0966	0.50	-1.9
1,106,221	+		PP_0966	0.53	-3.6
1,106,222	-		PP_0966	0.53	-3.5
1,106,222	-		PP_0966	0.53	-2.3