Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0786 and PP_0787 are separated by 75 nucleotides PP_0787 and PP_0788 are separated by 243 nucleotides
PP_0786: PP_0786 - thioredoxin reductase, at 901,612 to 902,574
_0786
PP_0787: PP_0787 - quinolinate phosphoribosyltransferase (decarboxylating) monomer, at 902,650 to 903,498
_0787
PP_0788: PP_0788 - conserved protein of unknown function, at 903,742 to 905,901
_0788
Position (kb) 902
903
904 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.717 kb on + strand at 902.718 kb on - strand at 902.718 kb on - strand at 902.718 kb on - strand at 902.718 kb on - strand at 902.800 kb on - strand, within PP_0787 at 903.008 kb on + strand, within PP_0787 at 903.009 kb on - strand, within PP_0787 at 903.016 kb on + strand, within PP_0787 at 903.016 kb on + strand, within PP_0787 at 903.017 kb on - strand, within PP_0787 at 903.487 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.515 kb on + strand at 903.516 kb on - strand at 903.516 kb on - strand at 903.516 kb on - strand at 903.516 kb on - strand at 903.530 kb on - strand at 903.531 kb on + strand at 903.531 kb on + strand at 903.532 kb on - strand at 903.551 kb on + strand at 903.551 kb on + strand at 903.551 kb on + strand at 903.552 kb on - strand at 903.552 kb on - strand at 903.552 kb on - strand at 903.552 kb on - strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.640 kb on + strand at 903.641 kb on - strand at 903.641 kb on - strand at 903.641 kb on - strand at 903.644 kb on + strand at 903.645 kb on - strand at 903.645 kb on - strand at 903.675 kb on + strand at 903.687 kb on + strand at 903.799 kb on - strand at 903.799 kb on - strand at 903.953 kb on - strand at 903.953 kb on - strand at 903.953 kb on - strand at 904.014 kb on + strand, within PP_0788 at 904.015 kb on - strand, within PP_0788 at 904.015 kb on - strand, within PP_0788 at 904.213 kb on + strand, within PP_0788 at 904.214 kb on - strand, within PP_0788 at 904.255 kb on + strand, within PP_0788 at 904.255 kb on + strand, within PP_0788 at 904.275 kb on + strand, within PP_0788 at 904.275 kb on + strand, within PP_0788 at 904.330 kb on + strand, within PP_0788 at 904.331 kb on - strand, within PP_0788 at 904.331 kb on - strand, within PP_0788 at 904.357 kb on + strand, within PP_0788 at 904.358 kb on - strand, within PP_0788 at 904.395 kb on - strand, within PP_0788
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 902,717 + -3.7 902,717 + -3.3 902,717 + -2.4 902,717 + -1.5 902,717 + -3.4 902,717 + -3.0 902,717 + -0.2 902,717 + -2.9 902,717 + -3.0 902,718 - -2.4 902,718 - -2.2 902,718 - +2.4 902,718 - -2.3 902,800 - PP_0787 0.18 -2.5 903,008 + PP_0787 0.42 -2.7 903,009 - PP_0787 0.42 -3.4 903,016 + PP_0787 0.43 -1.7 903,016 + PP_0787 0.43 -0.9 903,017 - PP_0787 0.43 -2.3 903,487 + +0.8 903,515 + +0.1 903,515 + -0.1 903,515 + +0.5 903,515 + -0.5 903,515 + -1.0 903,516 - -0.8 903,516 - -0.2 903,516 - -0.1 903,516 - +0.2 903,530 - -0.1 903,531 + +0.1 903,531 + -0.7 903,532 - +0.4 903,551 + -1.6 903,551 + -0.6 903,551 + +0.4 903,552 - -0.5 903,552 - -0.5 903,552 - -0.1 903,552 - -1.9 903,640 + -0.5 903,640 + -0.2 903,640 + +0.8 903,640 + -0.1 903,640 + -0.2 903,641 - +0.4 903,641 - +0.1 903,641 - +0.1 903,644 + +0.5 903,645 - -0.8 903,645 - -0.5 903,675 + +2.4 903,687 + +0.6 903,799 - +0.0 903,799 - +1.4 903,953 - +0.1 903,953 - +1.2 903,953 - -0.9 904,014 + PP_0788 0.13 +0.5 904,015 - PP_0788 0.13 +2.4 904,015 - PP_0788 0.13 +0.1 904,213 + PP_0788 0.22 +0.0 904,214 - PP_0788 0.22 +0.4 904,255 + PP_0788 0.24 -0.2 904,255 + PP_0788 0.24 -0.7 904,275 + PP_0788 0.25 -0.2 904,275 + PP_0788 0.25 -0.1 904,330 + PP_0788 0.27 +0.1 904,331 - PP_0788 0.27 -0.3 904,331 - PP_0788 0.27 -0.1 904,357 + PP_0788 0.28 -0.2 904,358 - PP_0788 0.29 -0.0 904,395 - PP_0788 0.30 -0.7
Or see this region's nucleotide sequence