Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0733 and PP_0734 overlap by 1 nucleotides PP_0734 and PP_0735 overlap by 7 nucleotides PP_0735 and PP_0736 overlap by 8 nucleotides PP_0736 and PP_0737 are separated by 104 nucleotides
PP_0733: PP_0733 - Peptide chain release factor 1, at 852,496 to 853,578
_0733
PP_0734: PP_0734 - release factor-(glutamine-N5) methyltransferase, at 853,578 to 854,408
_0734
PP_0735: PP_0735 - molybdopterin-synthase adenylyltransferase, at 854,402 to 855,157
_0735
PP_0736: PP_0736 - Glutamate racemase, at 855,150 to 855,947
_0736
PP_0737: PP_0737 - lipid A 3-O-deacylase, at 856,052 to 856,570
_0737
Position (kb) 854
855
856 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 854.903 kb on + strand, within PP_0735 at 854.903 kb on + strand, within PP_0735 at 854.915 kb on + strand at 855.142 kb on + strand at 855.142 kb on + strand at 855.566 kb on - strand, within PP_0736 at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.972 kb on + strand at 855.973 kb on - strand at 855.994 kb on - strand at 855.994 kb on - strand at 856.023 kb on + strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 854,903 + PP_0735 0.66 -2.5 854,903 + PP_0735 0.66 -3.0 854,915 + +0.4 855,142 + +0.6 855,142 + -1.3 855,566 - PP_0736 0.52 -1.3 855,915 - -1.1 855,915 - +1.0 855,915 - -0.8 855,915 - -0.3 855,972 + +0.4 855,973 - +1.2 855,994 - -0.0 855,994 - +1.3 856,023 + +2.4 856,024 - +0.4 856,024 - -1.1 856,024 - -0.3 856,052 - -0.7
Or see this region's nucleotide sequence