Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0690 and PP_0691 are separated by 85 nucleotides PP_0691 and PP_0692 overlap by 4 nucleotides PP_0692 and PP_0693 are separated by 93 nucleotides
PP_0690: PP_0690 - GTPase Obg, at 801,516 to 802,742
_0690
PP_0691: PP_0691 - Glutamate 5-kinase, at 802,828 to 803,946
_0691
PP_0692: PP_0692 - conserved protein of unknown function, at 803,943 to 804,404
_0692
PP_0693: PP_0693 - conserved protein of unknown function, at 804,498 to 807,329
_0693
Position (kb) 802
803
804 Strain fitness (log2 ratio) -2
-1
0
1
2 at 802.728 kb on + strand at 802.728 kb on + strand at 802.728 kb on + strand at 802.753 kb on + strand at 802.788 kb on + strand at 802.798 kb on - strand at 802.801 kb on + strand at 802.801 kb on + strand at 802.802 kb on - strand at 802.802 kb on - strand at 802.814 kb on + strand at 802.814 kb on + strand at 802.866 kb on + strand at 802.976 kb on + strand, within PP_0691 at 803.358 kb on + strand, within PP_0691 at 803.359 kb on - strand, within PP_0691 at 803.359 kb on - strand, within PP_0691 at 803.359 kb on - strand, within PP_0691 at 803.359 kb on - strand at 803.405 kb on - strand, within PP_0691 at 803.830 kb on + strand, within PP_0691 at 803.831 kb on - strand, within PP_0691 at 803.831 kb on - strand, within PP_0691 at 803.896 kb on + strand at 803.896 kb on + strand at 803.897 kb on - strand at 803.899 kb on - strand at 803.918 kb on + strand at 803.942 kb on + strand at 803.962 kb on + strand at 803.962 kb on + strand at 803.962 kb on + strand at 804.087 kb on - strand, within PP_0692 at 804.109 kb on - strand, within PP_0692 at 804.311 kb on + strand, within PP_0692 at 804.311 kb on + strand, within PP_0692 at 804.311 kb on + strand, within PP_0692 at 804.312 kb on - strand, within PP_0692 at 804.312 kb on - strand, within PP_0692 at 804.321 kb on + strand, within PP_0692 at 804.321 kb on + strand, within PP_0692 at 804.322 kb on - strand, within PP_0692 at 804.330 kb on + strand, within PP_0692 at 804.405 kb on + strand at 804.406 kb on - strand at 804.406 kb on - strand at 804.420 kb on + strand at 804.421 kb on - strand at 804.421 kb on - strand at 804.421 kb on - strand at 804.451 kb on - strand at 804.451 kb on - strand at 804.451 kb on - strand at 804.586 kb on + strand at 804.586 kb on + strand at 804.586 kb on + strand at 804.587 kb on - strand at 804.587 kb on - strand at 804.745 kb on + strand at 804.746 kb on - strand at 804.746 kb on - strand at 804.748 kb on + strand at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.802 kb on + strand, within PP_0693 at 804.803 kb on - strand, within PP_0693 at 804.803 kb on - strand, within PP_0693 at 804.803 kb on - strand, within PP_0693
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 802,728 + +1.4 802,728 + +1.4 802,728 + -0.8 802,753 + -0.1 802,788 + -0.6 802,798 - +1.4 802,801 + +0.5 802,801 + +0.6 802,802 - -1.2 802,802 - -1.1 802,814 + +0.6 802,814 + -0.1 802,866 + -0.8 802,976 + PP_0691 0.13 +0.8 803,358 + PP_0691 0.47 +0.4 803,359 - PP_0691 0.47 -1.6 803,359 - PP_0691 0.47 -0.2 803,359 - PP_0691 0.47 +1.4 803,359 - +0.8 803,405 - PP_0691 0.52 +0.1 803,830 + PP_0691 0.90 -1.3 803,831 - PP_0691 0.90 -0.9 803,831 - PP_0691 0.90 -0.4 803,896 + -0.6 803,896 + -0.4 803,897 - -0.2 803,899 - +0.1 803,918 + -2.2 803,942 + +0.9 803,962 + -0.4 803,962 + -0.4 803,962 + +2.4 804,087 - PP_0692 0.31 -0.9 804,109 - PP_0692 0.36 +1.0 804,311 + PP_0692 0.80 +0.7 804,311 + PP_0692 0.80 +0.4 804,311 + PP_0692 0.80 +0.4 804,312 - PP_0692 0.80 +0.2 804,312 - PP_0692 0.80 -1.0 804,321 + PP_0692 0.82 +0.1 804,321 + PP_0692 0.82 -0.2 804,322 - PP_0692 0.82 +0.0 804,330 + PP_0692 0.84 -0.0 804,405 + +0.3 804,406 - -0.4 804,406 - -1.4 804,420 + +0.2 804,421 - -1.2 804,421 - -1.0 804,421 - -0.3 804,451 - -0.9 804,451 - +0.8 804,451 - +0.0 804,586 + -0.6 804,586 + +1.1 804,586 + +1.2 804,587 - -0.8 804,587 - +1.8 804,745 + -0.4 804,746 - -1.2 804,746 - +0.1 804,748 + -0.2 804,802 + PP_0693 0.11 -0.9 804,802 + PP_0693 0.11 +1.1 804,802 + PP_0693 0.11 +0.4 804,802 + PP_0693 0.11 -0.7 804,802 + PP_0693 0.11 +0.1 804,803 - PP_0693 0.11 -0.5 804,803 - PP_0693 0.11 -1.1 804,803 - PP_0693 0.11 +0.8
Or see this region's nucleotide sequence