Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0620 and PP_0621 are separated by 266 nucleotides PP_0621 and PP_0622 are separated by 13 nucleotides PP_0622 and PP_0623 are separated by 147 nucleotides
PP_0620: PP_0620 - GntR family transcriptional regulator, at 725,989 to 726,753
_0620
PP_0621: PP_0621 - conserved protein of unknown function, at 727,020 to 727,331
_0621
PP_0622: PP_0622 - Outer membrane protein assembly factor BamD, at 727,345 to 728,364
_0622
PP_0623: PP_0623 - 23S rRNA pseudouridine(1911,1915,1917) synthase, at 728,512 to 729,474
_0623
Position (kb) 727
728
729 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 726.377 kb on + strand, within PP_0620 at 726.446 kb on + strand, within PP_0620 at 726.446 kb on + strand, within PP_0620 at 726.447 kb on - strand, within PP_0620 at 726.447 kb on - strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.482 kb on + strand, within PP_0620 at 726.483 kb on - strand, within PP_0620 at 726.839 kb on + strand at 726.840 kb on - strand at 726.840 kb on - strand at 726.840 kb on - strand at 726.867 kb on + strand at 727.066 kb on + strand, within PP_0621 at 727.066 kb on + strand, within PP_0621 at 727.066 kb on + strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.067 kb on - strand, within PP_0621 at 727.189 kb on + strand, within PP_0621 at 727.189 kb on + strand, within PP_0621 at 727.190 kb on - strand, within PP_0621 at 727.370 kb on + strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.371 kb on - strand at 727.444 kb on - strand at 727.643 kb on + strand, within PP_0622 at 728.416 kb on - strand at 728.416 kb on - strand at 728.416 kb on - strand at 728.771 kb on - strand, within PP_0623 at 729.019 kb on + strand, within PP_0623 at 729.019 kb on + strand, within PP_0623 at 729.266 kb on - strand, within PP_0623 at 729.283 kb on - strand, within PP_0623
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 726,377 + PP_0620 0.51 -0.5 726,446 + PP_0620 0.60 +1.1 726,446 + PP_0620 0.60 -0.3 726,447 - PP_0620 0.60 -1.4 726,447 - PP_0620 0.60 +0.5 726,482 + PP_0620 0.64 +0.6 726,482 + PP_0620 0.64 +0.1 726,482 + PP_0620 0.64 +0.9 726,482 + PP_0620 0.64 -0.1 726,483 - PP_0620 0.65 +1.1 726,839 + +1.3 726,840 - -0.9 726,840 - +0.5 726,840 - -2.2 726,867 + -2.0 727,066 + PP_0621 0.15 -1.7 727,066 + PP_0621 0.15 -0.2 727,066 + PP_0621 0.15 -2.3 727,067 - PP_0621 0.15 -1.2 727,067 - PP_0621 0.15 +0.7 727,067 - PP_0621 0.15 -0.3 727,189 + PP_0621 0.54 -1.2 727,189 + PP_0621 0.54 -1.7 727,190 - PP_0621 0.54 -1.1 727,370 + -0.9 727,371 - -1.4 727,371 - +3.0 727,371 - -0.1 727,371 - -0.3 727,444 - -0.6 727,643 + PP_0622 0.29 +2.4 728,416 - +2.4 728,416 - +2.4 728,416 - +2.4 728,771 - PP_0623 0.27 +0.1 729,019 + PP_0623 0.53 +2.4 729,019 + PP_0623 0.53 +2.4 729,266 - PP_0623 0.78 +2.4 729,283 - PP_0623 0.80 +1.4
Or see this region's nucleotide sequence