Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0591 and PP_0592 are separated by 85 nucleotides PP_0592 and PP_0593 are separated by 368 nucleotides PP_0593 and PP_0594 are separated by 9 nucleotides
PP_0591: PP_0591 - Adenine deaminase, at 689,689 to 690,636
_0591
PP_0592: PP_0592 - Oxidoreductase, short chain dehydrogenase/reductase family, at 690,722 to 691,408
_0592
PP_0593: PP_0593 - putative ribosome-binding factor, at 691,777 to 692,361
_0593
PP_0594: PP_0594 - Transcriptional regulator, TetR family, at 692,371 to 693,114
_0594
Position (kb) 690
691
692 Strain fitness (log2 ratio) -1
0
1
2 at 689.761 kb on - strand at 689.790 kb on - strand, within PP_0591 at 689.874 kb on + strand, within PP_0591 at 689.991 kb on + strand, within PP_0591 at 689.991 kb on + strand, within PP_0591 at 689.991 kb on + strand, within PP_0591 at 689.992 kb on - strand, within PP_0591 at 690.030 kb on - strand, within PP_0591 at 690.396 kb on - strand, within PP_0591 at 690.414 kb on - strand, within PP_0591 at 690.414 kb on - strand, within PP_0591 at 690.414 kb on - strand, within PP_0591 at 690.453 kb on - strand, within PP_0591 at 690.455 kb on + strand, within PP_0591 at 690.491 kb on + strand, within PP_0591 at 690.491 kb on + strand at 690.492 kb on - strand, within PP_0591 at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.632 kb on + strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.633 kb on - strand at 690.636 kb on + strand at 690.637 kb on - strand at 690.673 kb on + strand at 690.674 kb on - strand at 690.674 kb on - strand at 690.674 kb on - strand at 690.747 kb on + strand at 690.748 kb on - strand at 690.960 kb on + strand, within PP_0592 at 690.960 kb on + strand, within PP_0592 at 690.960 kb on + strand, within PP_0592 at 690.961 kb on - strand, within PP_0592 at 690.961 kb on - strand, within PP_0592 at 691.044 kb on - strand, within PP_0592 at 691.124 kb on + strand, within PP_0592 at 691.175 kb on + strand, within PP_0592 at 691.176 kb on - strand, within PP_0592 at 691.176 kb on - strand, within PP_0592 at 691.176 kb on - strand, within PP_0592 at 691.402 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.450 kb on + strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.451 kb on - strand at 691.465 kb on - strand at 691.465 kb on - strand at 691.575 kb on + strand at 691.575 kb on + strand at 691.576 kb on - strand at 691.576 kb on - strand at 691.576 kb on - strand at 691.576 kb on - strand at 691.631 kb on + strand at 691.631 kb on + strand at 691.632 kb on - strand at 691.654 kb on + strand at 691.682 kb on + strand at 691.714 kb on + strand at 691.740 kb on + strand at 691.797 kb on + strand at 691.798 kb on - strand at 691.798 kb on - strand at 691.798 kb on - strand at 691.798 kb on - strand at 692.039 kb on + strand, within PP_0593 at 692.331 kb on - strand at 692.352 kb on + strand at 692.352 kb on + strand at 692.352 kb on + strand at 692.353 kb on - strand at 692.353 kb on - strand at 692.361 kb on + strand at 692.361 kb on + strand at 692.362 kb on - strand at 692.362 kb on - strand at 692.362 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 689,761 - +0.3 689,790 - PP_0591 0.11 +0.1 689,874 + PP_0591 0.20 +1.8 689,991 + PP_0591 0.32 -0.1 689,991 + PP_0591 0.32 -0.0 689,991 + PP_0591 0.32 +0.2 689,992 - PP_0591 0.32 +0.3 690,030 - PP_0591 0.36 +0.5 690,396 - PP_0591 0.75 -0.3 690,414 - PP_0591 0.76 +0.0 690,414 - PP_0591 0.76 -0.7 690,414 - PP_0591 0.76 +0.5 690,453 - PP_0591 0.81 -0.5 690,455 + PP_0591 0.81 +0.3 690,491 + PP_0591 0.85 -0.8 690,491 + +2.4 690,492 - PP_0591 0.85 +0.9 690,632 + +0.1 690,632 + -1.0 690,632 + -0.3 690,632 + -0.2 690,632 + -1.5 690,632 + +1.1 690,632 + -0.5 690,632 + +0.4 690,632 + +0.8 690,632 + +0.5 690,632 + -0.2 690,632 + -0.8 690,633 - -0.6 690,633 - +0.3 690,633 - -0.8 690,633 - -0.7 690,633 - -0.1 690,633 - -0.5 690,633 - +1.5 690,633 - +1.1 690,633 - +0.0 690,633 - +0.5 690,633 - -1.5 690,633 - -0.2 690,633 - +1.0 690,636 + -0.1 690,637 - -0.9 690,673 + +0.7 690,674 - -0.3 690,674 - +0.3 690,674 - +1.0 690,747 + +0.3 690,748 - -1.1 690,960 + PP_0592 0.35 -1.5 690,960 + PP_0592 0.35 -0.8 690,960 + PP_0592 0.35 +1.0 690,961 - PP_0592 0.35 -0.6 690,961 - PP_0592 0.35 -0.5 691,044 - PP_0592 0.47 -1.3 691,124 + PP_0592 0.59 -0.8 691,175 + PP_0592 0.66 -0.1 691,176 - PP_0592 0.66 -1.0 691,176 - PP_0592 0.66 -0.4 691,176 - PP_0592 0.66 -0.3 691,402 + -0.1 691,450 + +0.2 691,450 + -1.0 691,450 + +0.5 691,450 + +1.0 691,450 + +0.0 691,450 + -1.2 691,451 - +0.1 691,451 - +0.6 691,451 - +0.7 691,451 - -0.3 691,451 - +0.4 691,465 - -0.3 691,465 - +1.0 691,575 + +0.2 691,575 + +0.5 691,576 - +1.1 691,576 - -0.1 691,576 - +0.5 691,576 - -0.0 691,631 + +0.6 691,631 + -0.6 691,632 - -1.2 691,654 + -0.2 691,682 + -1.3 691,714 + +0.8 691,740 + +0.6 691,797 + -0.4 691,798 - +0.6 691,798 - -0.3 691,798 - +2.4 691,798 - +0.1 692,039 + PP_0593 0.45 -0.4 692,331 - -0.4 692,352 + -0.6 692,352 + -1.0 692,352 + +0.0 692,353 - +0.1 692,353 - -0.5 692,361 + -1.1 692,361 + +1.4 692,362 - +0.8 692,362 - +0.9 692,362 - +0.0
Or see this region's nucleotide sequence