Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0506 and PP_0507 overlap by 1 nucleotides PP_0507 and PP_0508 are separated by 0 nucleotides PP_0508 and PP_0509 are separated by 130 nucleotides PP_0509 and PP_0510 are separated by 151 nucleotides
PP_0506: PP_0506 - putative ABC efflux transporter, permease protein, at 594,888 to 596,153
_0506
PP_0507: PP_0507 - putative ABC efflux transporter, ATP-binding protein, at 596,153 to 596,863
_0507
PP_0508: PP_0508 - conserved exported protein of unknown function, at 596,864 to 597,445
_0508
PP_0509: PP_0509 - conserved protein of unknown function, at 597,576 to 597,920
_0509
PP_0510: PP_0510 - Thioredoxin, at 598,072 to 598,944
_0510
Position (kb)
596
597
598 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 595.888 kb on + strand, within PP_0506 at 595.888 kb on + strand, within PP_0506 at 595.889 kb on - strand, within PP_0506 at 595.889 kb on - strand, within PP_0506 at 595.889 kb on - strand, within PP_0506 at 595.916 kb on + strand, within PP_0506 at 595.916 kb on + strand, within PP_0506 at 595.917 kb on - strand, within PP_0506 at 595.948 kb on + strand, within PP_0506 at 595.948 kb on + strand, within PP_0506 at 595.949 kb on - strand, within PP_0506 at 596.150 kb on - strand at 596.562 kb on - strand, within PP_0507 at 596.599 kb on + strand, within PP_0507 at 596.599 kb on + strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.602 kb on - strand, within PP_0507 at 596.602 kb on - strand, within PP_0507 at 597.024 kb on + strand, within PP_0508 at 597.025 kb on - strand, within PP_0508 at 597.025 kb on - strand, within PP_0508 at 597.295 kb on + strand, within PP_0508 at 597.327 kb on - strand, within PP_0508 at 597.630 kb on - strand, within PP_0509 at 597.630 kb on - strand, within PP_0509 at 597.630 kb on - strand, within PP_0509 at 597.630 kb on - strand, within PP_0509 at 597.679 kb on + strand, within PP_0509 at 597.904 kb on - strand at 597.904 kb on - strand at 598.192 kb on - strand, within PP_0510 at 598.232 kb on + strand, within PP_0510 at 598.233 kb on - strand, within PP_0510 at 598.311 kb on + strand, within PP_0510 at 598.311 kb on + strand, within PP_0510
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 595,888 + PP_0506 0.79 +0.4 595,888 + PP_0506 0.79 -2.1 595,889 - PP_0506 0.79 +0.2 595,889 - PP_0506 0.79 -1.1 595,889 - PP_0506 0.79 +0.1 595,916 + PP_0506 0.81 -1.0 595,916 + PP_0506 0.81 -0.9 595,917 - PP_0506 0.81 -1.7 595,948 + PP_0506 0.84 +1.8 595,948 + PP_0506 0.84 +0.4 595,949 - PP_0506 0.84 +0.6 596,150 - -0.7 596,562 - PP_0507 0.58 -0.8 596,599 + PP_0507 0.63 -0.2 596,599 + PP_0507 0.63 +0.6 596,600 - PP_0507 0.63 -1.8 596,600 - PP_0507 0.63 -0.9 596,600 - PP_0507 0.63 -0.9 596,600 - PP_0507 0.63 -1.1 596,600 - PP_0507 0.63 +1.4 596,601 + PP_0507 0.63 -3.1 596,601 + PP_0507 0.63 -1.9 596,601 + PP_0507 0.63 +2.4 596,601 + PP_0507 0.63 +0.4 596,602 - PP_0507 0.63 -1.3 596,602 - PP_0507 0.63 -0.9 597,024 + PP_0508 0.27 -1.4 597,025 - PP_0508 0.28 -1.6 597,025 - PP_0508 0.28 -0.6 597,295 + PP_0508 0.74 +0.2 597,327 - PP_0508 0.80 -0.7 597,630 - PP_0509 0.16 -0.6 597,630 - PP_0509 0.16 -0.7 597,630 - PP_0509 0.16 +0.9 597,630 - PP_0509 0.16 +0.1 597,679 + PP_0509 0.30 -0.5 597,904 - -1.1 597,904 - +0.3 598,192 - PP_0510 0.14 -0.5 598,232 + PP_0510 0.18 -0.4 598,233 - PP_0510 0.18 +1.4 598,311 + PP_0510 0.27 -1.6 598,311 + PP_0510 0.27 +0.4
Or see this region's nucleotide sequence