Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0506 and PP_0507 overlap by 1 nucleotides PP_0507 and PP_0508 are separated by 0 nucleotides PP_0508 and PP_0509 are separated by 130 nucleotides
PP_0506: PP_0506 - putative ABC efflux transporter, permease protein, at 594,888 to 596,153
_0506
PP_0507: PP_0507 - putative ABC efflux transporter, ATP-binding protein, at 596,153 to 596,863
_0507
PP_0508: PP_0508 - conserved exported protein of unknown function, at 596,864 to 597,445
_0508
PP_0509: PP_0509 - conserved protein of unknown function, at 597,576 to 597,920
_0509
Position (kb) 596
597 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 595.264 kb on - strand, within PP_0506 at 595.335 kb on + strand, within PP_0506 at 595.335 kb on + strand, within PP_0506 at 595.336 kb on - strand, within PP_0506 at 595.336 kb on - strand, within PP_0506 at 595.501 kb on - strand, within PP_0506 at 595.551 kb on - strand, within PP_0506 at 595.590 kb on + strand, within PP_0506 at 595.605 kb on - strand, within PP_0506 at 595.681 kb on + strand, within PP_0506 at 595.681 kb on + strand, within PP_0506 at 595.681 kb on + strand, within PP_0506 at 595.682 kb on - strand, within PP_0506 at 595.781 kb on - strand, within PP_0506 at 595.781 kb on - strand, within PP_0506 at 595.781 kb on - strand, within PP_0506 at 595.786 kb on + strand, within PP_0506 at 595.786 kb on + strand, within PP_0506 at 595.888 kb on + strand, within PP_0506 at 595.888 kb on + strand, within PP_0506 at 595.889 kb on - strand, within PP_0506 at 595.889 kb on - strand, within PP_0506 at 595.889 kb on - strand, within PP_0506 at 595.916 kb on + strand, within PP_0506 at 595.916 kb on + strand, within PP_0506 at 595.917 kb on - strand, within PP_0506 at 595.948 kb on + strand, within PP_0506 at 595.948 kb on + strand, within PP_0506 at 595.949 kb on - strand, within PP_0506 at 596.150 kb on - strand at 596.562 kb on - strand, within PP_0507 at 596.599 kb on + strand, within PP_0507 at 596.599 kb on + strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.600 kb on - strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.601 kb on + strand, within PP_0507 at 596.602 kb on - strand, within PP_0507 at 596.602 kb on - strand, within PP_0507 at 597.024 kb on + strand, within PP_0508 at 597.025 kb on - strand, within PP_0508 at 597.025 kb on - strand, within PP_0508 at 597.295 kb on + strand, within PP_0508 at 597.327 kb on - strand, within PP_0508 at 597.630 kb on - strand, within PP_0509 at 597.630 kb on - strand, within PP_0509 at 597.630 kb on - strand, within PP_0509 at 597.630 kb on - strand, within PP_0509 at 597.679 kb on + strand, within PP_0509
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 595,264 - PP_0506 0.30 -1.3 595,335 + PP_0506 0.35 -1.2 595,335 + PP_0506 0.35 +0.1 595,336 - PP_0506 0.35 +2.4 595,336 - PP_0506 0.35 -2.5 595,501 - PP_0506 0.48 -1.3 595,551 - PP_0506 0.52 -1.1 595,590 + PP_0506 0.55 +0.7 595,605 - PP_0506 0.57 -0.8 595,681 + PP_0506 0.63 +0.4 595,681 + PP_0506 0.63 -0.8 595,681 + PP_0506 0.63 -1.8 595,682 - PP_0506 0.63 +1.4 595,781 - PP_0506 0.71 -2.4 595,781 - PP_0506 0.71 +0.4 595,781 - PP_0506 0.71 -0.7 595,786 + PP_0506 0.71 +1.2 595,786 + PP_0506 0.71 -1.4 595,888 + PP_0506 0.79 +0.4 595,888 + PP_0506 0.79 -2.1 595,889 - PP_0506 0.79 +0.2 595,889 - PP_0506 0.79 -1.1 595,889 - PP_0506 0.79 +0.1 595,916 + PP_0506 0.81 -1.0 595,916 + PP_0506 0.81 -0.9 595,917 - PP_0506 0.81 -1.7 595,948 + PP_0506 0.84 +1.8 595,948 + PP_0506 0.84 +0.4 595,949 - PP_0506 0.84 +0.6 596,150 - -0.7 596,562 - PP_0507 0.58 -0.8 596,599 + PP_0507 0.63 -0.2 596,599 + PP_0507 0.63 +0.6 596,600 - PP_0507 0.63 -1.8 596,600 - PP_0507 0.63 -0.9 596,600 - PP_0507 0.63 -0.9 596,600 - PP_0507 0.63 -1.1 596,600 - PP_0507 0.63 +1.4 596,601 + PP_0507 0.63 -3.1 596,601 + PP_0507 0.63 -1.9 596,601 + PP_0507 0.63 +2.4 596,601 + PP_0507 0.63 +0.4 596,602 - PP_0507 0.63 -1.3 596,602 - PP_0507 0.63 -0.9 597,024 + PP_0508 0.27 -1.4 597,025 - PP_0508 0.28 -1.6 597,025 - PP_0508 0.28 -0.6 597,295 + PP_0508 0.74 +0.2 597,327 - PP_0508 0.80 -0.7 597,630 - PP_0509 0.16 -0.6 597,630 - PP_0509 0.16 -0.7 597,630 - PP_0509 0.16 +0.9 597,630 - PP_0509 0.16 +0.1 597,679 + PP_0509 0.30 -0.5
Or see this region's nucleotide sequence