Strain Fitness in Pseudomonas putida KT2440 around PP_0482

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
565,220	-		PP_0481	0.49	-1.0
565,232	+		PP_0481	0.49	-2.0
565,427	+		PP_0481	0.63	+1.1
565,468	+		PP_0481	0.66	+0.1
565,468	+		PP_0481	0.66	+0.2
565,469	-		PP_0481	0.66	-1.7
565,469	-		PP_0481	0.66	-0.5
565,469	-		PP_0481	0.66	-1.7
565,518	+		PP_0481	0.69	-0.9
565,519	-		PP_0481	0.69	+0.6
565,526	+		PP_0481	0.70	+1.0
565,607	+		PP_0481	0.75	+0.2
565,607	+		PP_0481	0.75	+0.1
565,608	-		PP_0481	0.75	+2.4
565,640	+		PP_0481	0.78	-1.1
565,641	-		PP_0481	0.78	+0.1
565,641	-		PP_0481	0.78	-0.9
565,641	-		PP_0481	0.78	-0.7
565,641	-		PP_0481	0.78	+0.3
565,645	+		PP_0481	0.78	-1.5
565,673	+		PP_0481	0.80	+0.8
565,762	+		PP_0481	0.86	-0.4
565,769	+		PP_0481	0.87	+0.6
565,770	-		PP_0481	0.87	+0.7
565,834	+				+0.6
565,834	+				-1.5
565,887	+				-0.7
565,887	+				+1.0
565,887	+				+0.9
565,892	+				-0.9
565,892	+				+1.8
565,893	-				+0.3
565,917	-				-0.3
566,010	+				+0.5
566,011	-				-0.1
566,066	-				-1.4
566,066	-				+1.8
566,086	+				+1.4
566,086	+				+0.1
566,086	+				-1.4
566,086	+				+0.1
566,087	-				+0.8
566,095	+				+0.2
566,095	+				-0.2
566,096	-				-0.4
566,143	+				-1.5
566,144	-				+0.8
566,144	-				-0.8
566,144	-				-1.9
566,144	-				-0.4
566,151	+				-2.6
566,151	+				-2.0
566,152	-				-2.0
566,152	-				-0.7
566,196	+		PP_0482	0.15	+0.7
566,196	+		PP_0482	0.15	-0.6
566,196	+		PP_0482	0.15	-1.0
566,217	+		PP_0482	0.20	-0.2
566,218	-		PP_0482	0.20	-1.0
566,218	-		PP_0482	0.20	-2.3
566,244	+		PP_0482	0.26	-0.6
566,360	+		PP_0482	0.51	-1.2
566,361	-		PP_0482	0.51	-2.8
566,403	+		PP_0482	0.60	+1.0
566,403	+		PP_0482	0.60	-0.7
566,404	-		PP_0482	0.60	+2.4
566,404	-		PP_0482	0.60	-1.1
566,460	+		PP_0482	0.72	+0.1
566,460	+		PP_0482	0.72	-0.8
566,460	+		PP_0482	0.72	-1.0
566,460	+		PP_0482	0.72	-0.5
566,460	+		PP_0482	0.72	-1.4
566,461	-		PP_0482	0.72	-0.7
566,461	-		PP_0482	0.72	-2.7
566,461	-		PP_0482	0.72	-0.4
566,517	+		PP_0482	0.85	-1.8
566,518	-		PP_0482	0.85	-0.9
566,518	-		PP_0482	0.85	+0.5
566,586	+				+2.4
566,586	+				-1.8
566,586	+				-0.6
566,586	+				-0.6
566,587	-				-0.9
566,587	-				+0.0
566,587	-				-0.3
566,587	-				+0.2
566,587	-				-2.5
566,587	-				-0.4
566,607	+				-0.7
566,630	-				-0.2
566,659	-				+0.7
567,220	+		PP_0483	0.20	+0.8