Strain Fitness in Pseudomonas putida KT2440 around PP_0420

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0418 and PP_0420 are separated by 367 nucleotidesPP_0420 and PP_0421 are separated by 9 nucleotides PP_0418: PP_0418 - Esterase EstP, at 507,121 to 509,010 _0418 PP_0420: PP_0420 - aminodeoxychorismate synthase / para-aminobenzoate synthase multi-enzyme complex, at 509,378 to 509,971 _0420 PP_0421: PP_0421 - Anthranilate phosphoribosyltransferase, at 509,981 to 511,030 _0421 Position (kb) 509 510Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 508.417 kb on + strand, within PP_0418at 508.418 kb on - strand, within PP_0418at 508.436 kb on + strand, within PP_0418at 508.436 kb on + strand, within PP_0418at 508.436 kb on + strand, within PP_0418at 508.437 kb on - strand, within PP_0418at 508.437 kb on - strand, within PP_0418at 508.437 kb on - strand, within PP_0418at 508.513 kb on - strand, within PP_0418at 508.556 kb on + strand, within PP_0418at 508.559 kb on + strand, within PP_0418at 508.560 kb on - strand, within PP_0418at 508.560 kb on - strand, within PP_0418at 508.560 kb on - strand, within PP_0418at 508.719 kb on + strand, within PP_0418at 508.720 kb on - strand, within PP_0418at 508.720 kb on - strand, within PP_0418at 508.722 kb on + strand, within PP_0418at 508.723 kb on - strand, within PP_0418at 508.723 kb on - strand, within PP_0418at 508.737 kb on + strand, within PP_0418at 508.738 kb on - strand, within PP_0418at 508.741 kb on + strand, within PP_0418at 508.741 kb on + strand, within PP_0418at 508.741 kb on + strand, within PP_0418at 508.741 kb on + strand, within PP_0418at 508.742 kb on - strand, within PP_0418at 508.742 kb on - strand, within PP_0418at 508.929 kb on - strandat 509.036 kb on + strandat 509.108 kb on - strandat 509.108 kb on - strandat 509.115 kb on + strandat 509.115 kb on + strandat 509.115 kb on + strandat 509.116 kb on - strandat 509.116 kb on - strandat 509.116 kb on - strandat 509.116 kb on - strandat 509.140 kb on + strandat 509.140 kb on + strandat 509.140 kb on + strandat 509.141 kb on - strandat 509.155 kb on + strandat 509.155 kb on + strandat 509.156 kb on - strandat 509.156 kb on - strandat 509.156 kb on - strandat 509.168 kb on + strandat 509.168 kb on + strandat 509.168 kb on + strandat 509.169 kb on - strandat 509.205 kb on + strandat 509.246 kb on + strandat 509.247 kb on - strandat 509.247 kb on - strandat 509.356 kb on + strandat 509.371 kb on + strandat 509.382 kb on + strandat 509.399 kb on + strandat 509.399 kb on + strandat 509.415 kb on - strandat 509.415 kb on - strandat 509.429 kb on + strandat 509.524 kb on - strand, within PP_0420at 509.524 kb on - strand, within PP_0420at 509.610 kb on + strand, within PP_0420at 509.610 kb on + strand, within PP_0420at 509.610 kb on + strand, within PP_0420at 509.610 kb on + strand, within PP_0420at 509.610 kb on + strand, within PP_0420at 509.610 kb on + strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.611 kb on - strand, within PP_0420at 509.658 kb on + strand, within PP_0420at 509.659 kb on - strand, within PP_0420at 509.697 kb on + strand, within PP_0420at 509.697 kb on + strand, within PP_0420at 509.697 kb on + strand, within PP_0420at 509.699 kb on + strand, within PP_0420at 509.700 kb on - strand, within PP_0420at 509.700 kb on - strand, within PP_0420at 509.700 kb on - strand, within PP_0420at 509.700 kb on - strand, within PP_0420at 509.700 kb on - strand, within PP_0420at 509.700 kb on - strand, within PP_0420at 509.731 kb on + strand, within PP_0420at 509.732 kb on - strand, within PP_0420at 509.772 kb on + strand, within PP_0420at 509.773 kb on - strand, within PP_0420at 510.032 kb on - strandat 510.093 kb on + strand, within PP_0421at 510.093 kb on + strand, within PP_0421at 510.316 kb on + strand, within PP_0421at 510.374 kb on + strand, within PP_0421at 510.374 kb on - strand, within PP_0421at 510.514 kb on + strand, within PP_0421at 510.514 kb on + strand, within PP_0421at 510.515 kb on - strand, within PP_0421at 510.529 kb on + strand, within PP_0421at 510.529 kb on + strand, within PP_0421at 510.529 kb on + strand, within PP_0421at 510.530 kb on - strand, within PP_0421at 510.722 kb on + strand, within PP_0421at 510.723 kb on - strand, within PP_0421at 510.874 kb on + strand, within PP_0421at 510.875 kb on - strand, within PP_0421at 510.875 kb on - strand, within PP_0421at 510.893 kb on + strand, within PP_0421at 510.893 kb on + strand, within PP_0421at 510.893 kb on + strand, within PP_0421at 510.893 kb on + strand, within PP_0421at 510.893 kb on + strand, within PP_0421at 510.894 kb on - strand, within PP_0421at 510.930 kb on + strandat 510.931 kb on - strandat 510.948 kb on + strandat 510.949 kb on - strandat 510.949 kb on - strandat 510.949 kb on - strandat 510.949 kb on - strandat 510.949 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
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508,417 + PP_0418 0.69 +0.4
508,418 - PP_0418 0.69 +0.7
508,436 + PP_0418 0.70 +1.5
508,436 + PP_0418 0.70 +0.8
508,436 + PP_0418 0.70 +2.0
508,437 - PP_0418 0.70 +0.9
508,437 - PP_0418 0.70 +0.8
508,437 - PP_0418 0.70 +0.1
508,513 - PP_0418 0.74 -0.4
508,556 + PP_0418 0.76 +1.0
508,559 + PP_0418 0.76 -0.2
508,560 - PP_0418 0.76 -0.4
508,560 - PP_0418 0.76 +0.8
508,560 - PP_0418 0.76 -0.6
508,719 + PP_0418 0.85 +0.2
508,720 - PP_0418 0.85 +0.1
508,720 - PP_0418 0.85 -0.5
508,722 + PP_0418 0.85 +1.0
508,723 - PP_0418 0.85 +0.4
508,723 - PP_0418 0.85 -1.3
508,737 + PP_0418 0.86 +1.7
508,738 - PP_0418 0.86 -0.2
508,741 + PP_0418 0.86 +0.2
508,741 + PP_0418 0.86 +0.8
508,741 + PP_0418 0.86 +0.9
508,741 + PP_0418 0.86 +0.4
508,742 - PP_0418 0.86 +1.0
508,742 - PP_0418 0.86 -0.8
508,929 - -1.3
509,036 + -0.0
509,108 - +1.1
509,108 - -1.6
509,115 + +0.2
509,115 + +2.4
509,115 + -0.9
509,116 - +0.3
509,116 - -0.4
509,116 - -0.0
509,116 - +0.4
509,140 + +0.2
509,140 + +0.0
509,140 + -0.8
509,141 - -2.1
509,155 + -0.5
509,155 + +0.9
509,156 - -1.4
509,156 - +0.8
509,156 - -3.6
509,168 + +1.4
509,168 + -1.1
509,168 + -1.6
509,169 - -3.4
509,205 + -0.5
509,246 + +1.2
509,247 - -1.0
509,247 - -3.4
509,356 + +0.8
509,371 + +0.8
509,382 + -2.3
509,399 + -1.9
509,399 + -2.5
509,415 - -3.2
509,415 - -2.9
509,429 + -3.4
509,524 - PP_0420 0.25 -1.8
509,524 - PP_0420 0.25 -3.9
509,610 + PP_0420 0.39 -2.9
509,610 + PP_0420 0.39 -1.1
509,610 + PP_0420 0.39 -1.7
509,610 + PP_0420 0.39 -3.3
509,610 + PP_0420 0.39 -2.1
509,610 + PP_0420 0.39 +1.4
509,611 - PP_0420 0.39 -3.0
509,611 - PP_0420 0.39 -2.5
509,611 - PP_0420 0.39 -3.6
509,611 - PP_0420 0.39 -0.4
509,611 - PP_0420 0.39 -3.1
509,611 - PP_0420 0.39 -3.6
509,611 - PP_0420 0.39 -2.6
509,611 - PP_0420 0.39 -3.0
509,611 - PP_0420 0.39 -3.5
509,658 + PP_0420 0.47 -1.2
509,659 - PP_0420 0.47 -1.2
509,697 + PP_0420 0.54 +0.4
509,697 + PP_0420 0.54 -1.4
509,697 + PP_0420 0.54 -1.1
509,699 + PP_0420 0.54 -3.1
509,700 - PP_0420 0.54 -2.8
509,700 - PP_0420 0.54 -1.7
509,700 - PP_0420 0.54 -2.6
509,700 - PP_0420 0.54 +0.1
509,700 - PP_0420 0.54 -2.4
509,700 - PP_0420 0.54 -2.5
509,731 + PP_0420 0.59 -0.9
509,732 - PP_0420 0.60 -1.8
509,772 + PP_0420 0.66 -2.1
509,773 - PP_0420 0.66 -2.2
510,032 - -3.0
510,093 + PP_0421 0.11 -2.9
510,093 + PP_0421 0.11 -2.2
510,316 + PP_0421 0.32 +1.4
510,374 + PP_0421 0.37 -1.5
510,374 - PP_0421 0.37 -2.0
510,514 + PP_0421 0.51 -0.8
510,514 + PP_0421 0.51 -2.3
510,515 - PP_0421 0.51 -0.2
510,529 + PP_0421 0.52 -3.9
510,529 + PP_0421 0.52 -0.6
510,529 + PP_0421 0.52 -1.2
510,530 - PP_0421 0.52 -0.2
510,722 + PP_0421 0.71 -3.2
510,723 - PP_0421 0.71 -1.4
510,874 + PP_0421 0.85 -1.2
510,875 - PP_0421 0.85 -2.9
510,875 - PP_0421 0.85 -1.8
510,893 + PP_0421 0.87 -3.5
510,893 + PP_0421 0.87 -1.6
510,893 + PP_0421 0.87 -3.4
510,893 + PP_0421 0.87 -1.4
510,893 + PP_0421 0.87 -2.3
510,894 - PP_0421 0.87 -1.3
510,930 + -3.3
510,931 - -3.3
510,948 + -2.4
510,949 - -2.2
510,949 - -1.6
510,949 - -0.8
510,949 - -0.2
510,949 - -2.7

Or see this region's nucleotide sequence