Strain Fitness in Pseudomonas putida KT2440 around PP_0362

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0360 and PP_0361 are separated by 49 nucleotidesPP_0361 and PP_0362 are separated by 89 nucleotidesPP_0362 and PP_0363 are separated by 1 nucleotides PP_0360: PP_0360 - Molybdate transport regulator, at 438,425 to 439,189 _0360 PP_0361: PP_0361 - putative Competence protein ComF, at 439,239 to 439,970 _0361 PP_0362: PP_0362 - Biotin synthase, at 440,060 to 441,118 _0362 PP_0363: PP_0363 - 8-amino-7-oxononanoate synthase, at 441,120 to 442,292 _0363 Position (kb) 440 441 442Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 439.069 kb on - strand, within PP_0360at 439.069 kb on - strand, within PP_0360at 439.076 kb on - strand, within PP_0360at 439.120 kb on + strandat 439.120 kb on + strandat 439.121 kb on - strandat 439.121 kb on - strandat 439.121 kb on - strandat 439.193 kb on + strandat 439.193 kb on + strandat 439.193 kb on + strandat 439.201 kb on - strandat 439.201 kb on - strandat 439.202 kb on + strandat 439.202 kb on + strandat 439.202 kb on + strandat 439.202 kb on + strandat 439.202 kb on + strandat 439.202 kb on + strandat 439.203 kb on - strandat 439.203 kb on - strandat 439.216 kb on - strandat 439.216 kb on - strandat 439.216 kb on - strandat 439.242 kb on + strandat 439.242 kb on + strandat 439.242 kb on + strandat 439.242 kb on + strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.243 kb on - strandat 439.246 kb on + strandat 439.246 kb on + strandat 439.262 kb on + strandat 439.262 kb on + strandat 439.263 kb on - strandat 439.276 kb on + strandat 439.276 kb on + strandat 439.276 kb on + strandat 439.276 kb on + strandat 439.276 kb on + strandat 439.277 kb on - strandat 439.277 kb on - strandat 439.277 kb on - strandat 439.277 kb on - strandat 439.621 kb on + strand, within PP_0361at 439.627 kb on + strand, within PP_0361at 439.627 kb on + strand, within PP_0361at 439.628 kb on - strand, within PP_0361at 439.628 kb on - strand, within PP_0361at 439.902 kb on + strandat 439.902 kb on + strandat 439.903 kb on - strandat 439.970 kb on + strandat 439.971 kb on - strandat 439.985 kb on + strandat 439.986 kb on - strandat 439.986 kb on - strandat 439.995 kb on - strandat 439.995 kb on - strandat 440.037 kb on - strandat 440.043 kb on + strandat 440.044 kb on - strandat 440.252 kb on + strand, within PP_0362at 440.252 kb on + strand, within PP_0362at 440.253 kb on - strand, within PP_0362at 440.253 kb on - strand, within PP_0362at 440.253 kb on - strand, within PP_0362at 440.273 kb on + strand, within PP_0362at 440.274 kb on - strand, within PP_0362at 440.356 kb on + strand, within PP_0362at 440.356 kb on + strand, within PP_0362at 440.357 kb on - strand, within PP_0362at 440.411 kb on + strand, within PP_0362at 440.411 kb on + strand, within PP_0362at 440.411 kb on + strand, within PP_0362at 440.412 kb on - strand, within PP_0362at 440.412 kb on - strand, within PP_0362at 440.412 kb on - strand, within PP_0362at 440.412 kb on - strand, within PP_0362at 440.522 kb on + strand, within PP_0362at 440.523 kb on - strand, within PP_0362at 440.525 kb on + strand, within PP_0362at 440.525 kb on + strand, within PP_0362at 440.525 kb on + strand, within PP_0362at 440.525 kb on + strand, within PP_0362at 440.525 kb on + strand, within PP_0362at 440.525 kb on + strand, within PP_0362at 440.526 kb on - strand, within PP_0362at 440.558 kb on + strand, within PP_0362at 440.581 kb on + strand, within PP_0362at 440.581 kb on + strand, within PP_0362at 440.582 kb on - strand, within PP_0362at 440.582 kb on - strand, within PP_0362at 440.585 kb on + strand, within PP_0362at 440.586 kb on - strand, within PP_0362at 440.586 kb on - strand, within PP_0362at 440.612 kb on + strand, within PP_0362at 440.612 kb on + strand, within PP_0362at 440.612 kb on + strand, within PP_0362at 440.613 kb on - strand, within PP_0362at 440.613 kb on - strand, within PP_0362at 440.613 kb on - strand, within PP_0362at 440.806 kb on + strand, within PP_0362at 440.807 kb on - strand, within PP_0362at 440.813 kb on - strand, within PP_0362at 440.813 kb on - strand, within PP_0362at 440.933 kb on + strand, within PP_0362at 441.098 kb on + strandat 441.099 kb on - strandat 441.258 kb on + strand, within PP_0363at 441.258 kb on + strand, within PP_0363at 441.259 kb on - strand, within PP_0363at 441.349 kb on + strand, within PP_0363at 441.350 kb on - strand, within PP_0363at 441.350 kb on - strand, within PP_0363at 441.438 kb on + strand, within PP_0363at 441.438 kb on + strand, within PP_0363at 441.438 kb on + strand, within PP_0363at 441.439 kb on - strand, within PP_0363at 441.439 kb on - strand, within PP_0363at 441.439 kb on - strand, within PP_0363at 441.439 kb on - strand, within PP_0363at 441.440 kb on + strand, within PP_0363at 441.440 kb on + strand, within PP_0363at 441.441 kb on - strand, within PP_0363at 441.618 kb on - strand, within PP_0363at 441.799 kb on + strand, within PP_0363at 441.799 kb on + strand, within PP_0363at 441.799 kb on + strand, within PP_0363at 441.800 kb on - strand, within PP_0363at 441.886 kb on + strand, within PP_0363at 441.886 kb on + strand, within PP_0363at 441.887 kb on - strand, within PP_0363at 441.903 kb on + strand, within PP_0363at 441.903 kb on + strand, within PP_0363at 441.905 kb on + strand, within PP_0363at 441.905 kb on + strand, within PP_0363at 441.905 kb on + strandat 441.905 kb on + strand, within PP_0363at 441.905 kb on + strand, within PP_0363at 441.905 kb on + strand, within PP_0363at 441.906 kb on - strand, within PP_0363at 441.906 kb on - strand, within PP_0363at 441.906 kb on - strand, within PP_0363at 441.906 kb on - strand, within PP_0363at 441.909 kb on + strand, within PP_0363at 441.909 kb on + strand, within PP_0363at 441.910 kb on - strand, within PP_0363at 441.910 kb on - strand, within PP_0363at 441.910 kb on - strand, within PP_0363at 441.910 kb on - strand, within PP_0363at 441.910 kb on - strand, within PP_0363at 441.910 kb on - strand, within PP_0363at 441.910 kb on - strand, within PP_0363

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Per-strain Table

Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
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439,069 - PP_0360 0.84 -0.2
439,069 - PP_0360 0.84 +0.2
439,076 - PP_0360 0.85 -1.1
439,120 + -0.3
439,120 + -0.3
439,121 - +0.0
439,121 - -1.1
439,121 - -0.1
439,193 + +1.6
439,193 + -0.5
439,193 + +1.0
439,201 - +0.2
439,201 - +0.1
439,202 + +0.4
439,202 + -0.3
439,202 + -0.3
439,202 + -0.1
439,202 + -0.5
439,202 + -0.2
439,203 - -0.3
439,203 - -2.0
439,216 - +0.4
439,216 - -0.1
439,216 - +0.2
439,242 + -0.8
439,242 + +1.1
439,242 + +0.7
439,242 + -0.5
439,243 - +0.7
439,243 - -1.1
439,243 - +1.1
439,243 - -1.5
439,243 - +0.1
439,243 - -1.2
439,243 - +0.4
439,243 - +0.0
439,243 - -0.4
439,243 - +0.1
439,243 - +0.6
439,246 + -2.1
439,246 + -1.5
439,262 + -1.5
439,262 + -1.5
439,263 - -0.6
439,276 + +0.9
439,276 + -0.7
439,276 + -0.6
439,276 + -0.7
439,276 + +2.4
439,277 - -0.9
439,277 - +0.2
439,277 - +0.1
439,277 - +0.2
439,621 + PP_0361 0.52 +0.3
439,627 + PP_0361 0.53 -1.3
439,627 + PP_0361 0.53 +0.8
439,628 - PP_0361 0.53 +0.4
439,628 - PP_0361 0.53 +0.8
439,902 + +0.2
439,902 + +2.4
439,903 - -2.6
439,970 + +1.7
439,971 - -3.4
439,985 + -1.4
439,986 - -2.0
439,986 - -2.1
439,995 - -1.2
439,995 - -1.4
440,037 - -2.2
440,043 + +0.1
440,044 - -2.1
440,252 + PP_0362 0.18 -1.3
440,252 + PP_0362 0.18 -2.3
440,253 - PP_0362 0.18 +0.3
440,253 - PP_0362 0.18 -0.3
440,253 - PP_0362 0.18 -1.7
440,273 + PP_0362 0.20 -2.8
440,274 - PP_0362 0.20 -1.1
440,356 + PP_0362 0.28 -2.6
440,356 + PP_0362 0.28 -0.2
440,357 - PP_0362 0.28 -2.4
440,411 + PP_0362 0.33 -2.4
440,411 + PP_0362 0.33 +0.2
440,411 + PP_0362 0.33 +0.1
440,412 - PP_0362 0.33 -3.0
440,412 - PP_0362 0.33 -3.0
440,412 - PP_0362 0.33 -1.1
440,412 - PP_0362 0.33 +0.8
440,522 + PP_0362 0.44 -2.5
440,523 - PP_0362 0.44 -2.5
440,525 + PP_0362 0.44 -0.8
440,525 + PP_0362 0.44 -3.4
440,525 + PP_0362 0.44 -1.3
440,525 + PP_0362 0.44 -1.7
440,525 + PP_0362 0.44 -2.3
440,525 + PP_0362 0.44 -2.7
440,526 - PP_0362 0.44 -1.3
440,558 + PP_0362 0.47 -1.3
440,581 + PP_0362 0.49 +2.4
440,581 + PP_0362 0.49 -3.6
440,582 - PP_0362 0.49 -4.1
440,582 - PP_0362 0.49 -1.8
440,585 + PP_0362 0.50 -1.9
440,586 - PP_0362 0.50 -2.2
440,586 - PP_0362 0.50 -3.4
440,612 + PP_0362 0.52 -1.8
440,612 + PP_0362 0.52 -1.2
440,612 + PP_0362 0.52 -1.6
440,613 - PP_0362 0.52 -1.5
440,613 - PP_0362 0.52 -3.5
440,613 - PP_0362 0.52 -3.4
440,806 + PP_0362 0.70 -1.4
440,807 - PP_0362 0.71 -1.3
440,813 - PP_0362 0.71 -2.8
440,813 - PP_0362 0.71 -2.9
440,933 + PP_0362 0.82 -2.6
441,098 + -0.4
441,099 - -0.4
441,258 + PP_0363 0.12 -1.0
441,258 + PP_0363 0.12 -0.8
441,259 - PP_0363 0.12 -1.1
441,349 + PP_0363 0.20 -0.8
441,350 - PP_0363 0.20 -0.2
441,350 - PP_0363 0.20 +0.6
441,438 + PP_0363 0.27 +0.2
441,438 + PP_0363 0.27 -1.2
441,438 + PP_0363 0.27 -0.4
441,439 - PP_0363 0.27 -0.7
441,439 - PP_0363 0.27 +0.6
441,439 - PP_0363 0.27 -0.0
441,439 - PP_0363 0.27 -0.1
441,440 + PP_0363 0.27 +0.5
441,440 + PP_0363 0.27 -1.8
441,441 - PP_0363 0.27 -0.6
441,618 - PP_0363 0.42 -0.8
441,799 + PP_0363 0.58 +0.0
441,799 + PP_0363 0.58 -0.3
441,799 + PP_0363 0.58 +0.2
441,800 - PP_0363 0.58 +2.4
441,886 + PP_0363 0.65 +0.4
441,886 + PP_0363 0.65 +0.3
441,887 - PP_0363 0.65 +0.3
441,903 + PP_0363 0.67 +1.4
441,903 + PP_0363 0.67 -0.6
441,905 + PP_0363 0.67 +2.4
441,905 + PP_0363 0.67 +0.4
441,905 + +1.4
441,905 + PP_0363 0.67 -0.7
441,905 + PP_0363 0.67 +0.1
441,905 + PP_0363 0.67 +0.0
441,906 - PP_0363 0.67 -0.3
441,906 - PP_0363 0.67 +0.6
441,906 - PP_0363 0.67 -0.7
441,906 - PP_0363 0.67 -0.9
441,909 + PP_0363 0.67 -1.4
441,909 + PP_0363 0.67 -0.7
441,910 - PP_0363 0.67 -1.4
441,910 - PP_0363 0.67 +0.5
441,910 - PP_0363 0.67 +0.7
441,910 - PP_0363 0.67 -0.5
441,910 - PP_0363 0.67 +3.4
441,910 - PP_0363 0.67 +0.8
441,910 - PP_0363 0.67 -1.7

Or see this region's nucleotide sequence