Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0291 and PP_0292 are separated by 27 nucleotides PP_0292 and PP_0293 are separated by 10 nucleotides PP_0293 and PP_0294 are separated by 117 nucleotides
PP_0291: PP_0291 - conserved protein of unknown function, at 351,227 to 351,487
_0291
PP_0292: PP_0292 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 351,515 to 352,252
_0292
PP_0293: PP_0293 - Imidazole glycerol phosphate synthase subunit HisF, at 352,263 to 353,033
_0293
PP_0294: PP_0294 - Choline / carnitine / glycine betaine ABC transporter, ATPase component <small>(from data)</small>, at 353,151 to 354,329
_0294
Position (kb) 352
353
354 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 351.396 kb on - strand, within PP_0291 at 351.396 kb on - strand, within PP_0291 at 351.398 kb on + strand, within PP_0291 at 351.398 kb on + strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.399 kb on - strand, within PP_0291 at 351.499 kb on + strand at 351.499 kb on + strand at 351.523 kb on + strand at 351.524 kb on - strand at 351.590 kb on - strand, within PP_0292 at 351.791 kb on + strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.792 kb on - strand, within PP_0292 at 351.812 kb on + strand at 351.813 kb on - strand, within PP_0292 at 351.813 kb on - strand, within PP_0292 at 352.193 kb on + strand at 352.193 kb on + strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.194 kb on - strand at 352.241 kb on + strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.242 kb on - strand at 352.457 kb on + strand, within PP_0293 at 352.457 kb on + strand, within PP_0293 at 352.457 kb on + strand, within PP_0293 at 352.977 kb on + strand at 352.977 kb on + strand at 352.978 kb on - strand at 353.001 kb on + strand at 353.002 kb on - strand at 353.002 kb on - strand at 353.427 kb on - strand, within PP_0294 at 353.519 kb on + strand, within PP_0294 at 353.542 kb on + strand, within PP_0294 at 353.542 kb on + strand, within PP_0294 at 353.543 kb on - strand, within PP_0294 at 353.543 kb on - strand, within PP_0294 at 353.618 kb on + strand, within PP_0294 at 353.618 kb on + strand, within PP_0294
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 351,396 - PP_0291 0.65 -2.0 351,396 - PP_0291 0.65 -1.1 351,398 + PP_0291 0.66 -0.4 351,398 + PP_0291 0.66 -0.1 351,399 - PP_0291 0.66 -2.2 351,399 - PP_0291 0.66 -2.5 351,399 - PP_0291 0.66 +0.8 351,399 - PP_0291 0.66 -1.4 351,499 + -0.7 351,499 + -1.9 351,523 + +2.4 351,524 - -1.3 351,590 - PP_0292 0.10 +0.1 351,791 + PP_0292 0.37 -0.9 351,792 - PP_0292 0.38 -2.1 351,792 - PP_0292 0.38 -0.8 351,792 - PP_0292 0.38 -1.9 351,812 + +0.8 351,813 - PP_0292 0.40 -1.5 351,813 - PP_0292 0.40 -2.8 352,193 + -1.9 352,193 + -1.5 352,194 - -0.4 352,194 - -0.8 352,194 - -1.5 352,194 - -0.9 352,241 + -1.5 352,242 - -2.3 352,242 - -1.8 352,242 - -1.4 352,242 - -1.8 352,242 - -2.1 352,242 - +0.1 352,457 + PP_0293 0.25 -2.0 352,457 + PP_0293 0.25 -1.2 352,457 + PP_0293 0.25 -2.3 352,977 + -1.5 352,977 + -1.5 352,978 - -0.3 353,001 + -1.2 353,002 - -2.3 353,002 - -3.1 353,427 - PP_0294 0.23 -0.2 353,519 + PP_0294 0.31 -0.0 353,542 + PP_0294 0.33 -1.2 353,542 + PP_0294 0.33 -0.6 353,543 - PP_0294 0.33 +0.4 353,543 - PP_0294 0.33 +0.4 353,618 + PP_0294 0.40 +1.4 353,618 + PP_0294 0.40 -1.5
Or see this region's nucleotide sequence