Strain Fitness in Pseudomonas putida KT2440 around PP_0290

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0288 and PP_0289 are separated by 179 nucleotidesPP_0289 and PP_0290 are separated by 0 nucleotidesPP_0290 and PP_0291 are separated by 0 nucleotidesPP_0291 and PP_0292 are separated by 27 nucleotides PP_0288: PP_0288 - putative Oxalate/formate antiporter, at 348,114 to 349,775 _0288 PP_0289: PP_0289 - Imidazoleglycerol-phosphate dehydratase, at 349,955 to 350,587 _0289 PP_0290: PP_0290 - Imidazole glycerol phosphate synthase subunit HisH, at 350,588 to 351,226 _0290 PP_0291: PP_0291 - conserved protein of unknown function, at 351,227 to 351,487 _0291 PP_0292: PP_0292 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data), at 351,515 to 352,252 _0292 Position (kb) 350 351 352Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 349.779 kb on - strandat 349.779 kb on - strandat 349.779 kb on - strandat 349.857 kb on + strandat 349.867 kb on + strandat 349.867 kb on + strandat 349.867 kb on + strandat 349.868 kb on - strandat 349.868 kb on - strandat 349.914 kb on + strandat 349.914 kb on + strandat 349.914 kb on + strandat 349.914 kb on + strandat 349.951 kb on + strandat 349.951 kb on + strandat 349.951 kb on + strandat 349.952 kb on - strandat 349.953 kb on + strandat 349.953 kb on + strandat 349.954 kb on - strandat 350.005 kb on + strandat 350.005 kb on + strandat 350.006 kb on - strandat 350.006 kb on - strandat 350.090 kb on - strand, within PP_0289at 350.174 kb on - strand, within PP_0289at 350.174 kb on - strand, within PP_0289at 350.174 kb on - strand, within PP_0289at 350.260 kb on + strand, within PP_0289at 350.260 kb on + strand, within PP_0289at 350.271 kb on - strand, within PP_0289at 350.282 kb on + strand, within PP_0289at 350.282 kb on + strand, within PP_0289at 350.282 kb on + strand, within PP_0289at 350.283 kb on - strand, within PP_0289at 350.355 kb on + strand, within PP_0289at 350.356 kb on - strand, within PP_0289at 350.356 kb on - strand, within PP_0289at 350.356 kb on - strand, within PP_0289at 350.360 kb on + strand, within PP_0289at 350.360 kb on + strand, within PP_0289at 350.361 kb on - strand, within PP_0289at 350.361 kb on - strand, within PP_0289at 350.361 kb on - strand, within PP_0289at 350.361 kb on - strand, within PP_0289at 350.449 kb on + strand, within PP_0289at 350.449 kb on + strand, within PP_0289at 350.548 kb on + strandat 350.548 kb on + strandat 350.548 kb on + strandat 350.549 kb on - strandat 350.585 kb on + strandat 350.612 kb on + strandat 350.658 kb on + strand, within PP_0290at 350.658 kb on + strand, within PP_0290at 350.659 kb on - strand, within PP_0290at 350.659 kb on - strand, within PP_0290at 350.668 kb on + strand, within PP_0290at 350.680 kb on + strand, within PP_0290at 351.035 kb on + strand, within PP_0290at 351.050 kb on + strand, within PP_0290at 351.050 kb on + strand, within PP_0290at 351.051 kb on - strand, within PP_0290at 351.051 kb on - strand, within PP_0290at 351.051 kb on - strand, within PP_0290at 351.053 kb on + strand, within PP_0290at 351.053 kb on + strand, within PP_0290at 351.053 kb on + strand, within PP_0290at 351.054 kb on - strand, within PP_0290at 351.054 kb on - strand, within PP_0290at 351.098 kb on + strand, within PP_0290at 351.099 kb on - strand, within PP_0290at 351.224 kb on + strandat 351.224 kb on + strandat 351.225 kb on - strandat 351.396 kb on - strand, within PP_0291at 351.396 kb on - strand, within PP_0291at 351.398 kb on + strand, within PP_0291at 351.398 kb on + strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.399 kb on - strand, within PP_0291at 351.499 kb on + strandat 351.499 kb on + strandat 351.523 kb on + strandat 351.524 kb on - strandat 351.590 kb on - strand, within PP_0292at 351.791 kb on + strand, within PP_0292at 351.792 kb on - strand, within PP_0292at 351.792 kb on - strand, within PP_0292at 351.792 kb on - strand, within PP_0292at 351.812 kb on + strandat 351.813 kb on - strand, within PP_0292at 351.813 kb on - strand, within PP_0292at 352.193 kb on + strandat 352.193 kb on + strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.194 kb on - strandat 352.194 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
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349,779 - +0.3
349,779 - -0.4
349,779 - -0.6
349,857 + -0.2
349,867 + -0.2
349,867 + +1.5
349,867 + +0.0
349,868 - +0.6
349,868 - -1.2
349,914 + -1.2
349,914 + -0.7
349,914 + -0.5
349,914 + +0.6
349,951 + +0.1
349,951 + -0.6
349,951 + +0.3
349,952 - -0.9
349,953 + -0.2
349,953 + -1.2
349,954 - -1.7
350,005 + -2.1
350,005 + -3.0
350,006 - -1.4
350,006 - -2.2
350,090 - PP_0289 0.21 +0.1
350,174 - PP_0289 0.35 -2.3
350,174 - PP_0289 0.35 -0.2
350,174 - PP_0289 0.35 -1.2
350,260 + PP_0289 0.48 -1.4
350,260 + PP_0289 0.48 -2.3
350,271 - PP_0289 0.50 -0.8
350,282 + PP_0289 0.52 -3.2
350,282 + PP_0289 0.52 -2.1
350,282 + PP_0289 0.52 -2.5
350,283 - PP_0289 0.52 -1.2
350,355 + PP_0289 0.63 -2.8
350,356 - PP_0289 0.63 -0.8
350,356 - PP_0289 0.63 -0.6
350,356 - PP_0289 0.63 +1.4
350,360 + PP_0289 0.64 -3.4
350,360 + PP_0289 0.64 -0.8
350,361 - PP_0289 0.64 -2.6
350,361 - PP_0289 0.64 -2.3
350,361 - PP_0289 0.64 +2.4
350,361 - PP_0289 0.64 -2.2
350,449 + PP_0289 0.78 -2.1
350,449 + PP_0289 0.78 -1.1
350,548 + +0.8
350,548 + +2.4
350,548 + -0.2
350,549 - -1.5
350,585 + -0.4
350,612 + -0.4
350,658 + PP_0290 0.11 -2.3
350,658 + PP_0290 0.11 -3.4
350,659 - PP_0290 0.11 -2.5
350,659 - PP_0290 0.11 -2.4
350,668 + PP_0290 0.13 -2.5
350,680 + PP_0290 0.14 -2.1
351,035 + PP_0290 0.70 -2.8
351,050 + PP_0290 0.72 -2.8
351,050 + PP_0290 0.72 -3.5
351,051 - PP_0290 0.72 -2.6
351,051 - PP_0290 0.72 -2.4
351,051 - PP_0290 0.72 +0.1
351,053 + PP_0290 0.73 -2.3
351,053 + PP_0290 0.73 -0.8
351,053 + PP_0290 0.73 -1.5
351,054 - PP_0290 0.73 -2.1
351,054 - PP_0290 0.73 -3.2
351,098 + PP_0290 0.80 -2.2
351,099 - PP_0290 0.80 -1.6
351,224 + +2.4
351,224 + -1.2
351,225 - -0.9
351,396 - PP_0291 0.65 -2.0
351,396 - PP_0291 0.65 -1.1
351,398 + PP_0291 0.66 -0.4
351,398 + PP_0291 0.66 -0.1
351,399 - PP_0291 0.66 -2.2
351,399 - PP_0291 0.66 -2.5
351,399 - PP_0291 0.66 +0.8
351,399 - PP_0291 0.66 -1.4
351,499 + -0.7
351,499 + -1.9
351,523 + +2.4
351,524 - -1.3
351,590 - PP_0292 0.10 +0.1
351,791 + PP_0292 0.37 -0.9
351,792 - PP_0292 0.38 -2.1
351,792 - PP_0292 0.38 -0.8
351,792 - PP_0292 0.38 -1.9
351,812 + +0.8
351,813 - PP_0292 0.40 -1.5
351,813 - PP_0292 0.40 -2.8
352,193 + -1.9
352,193 + -1.5
352,194 - -0.4
352,194 - -0.8
352,194 - -1.5
352,194 - -0.9

Or see this region's nucleotide sequence