Strain Fitness in Pseudomonas putida KT2440 around PP_0185

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0184 and PP_0185 are separated by 285 nucleotidesPP_0185 and PP_0186 are separated by 118 nucleotides PP_0184: PP_0184 - Argininosuccinate lyase, at 235,428 to 236,834 _0184 PP_0185: PP_0185 - Positive alginate biosynthesis regulatory protein, at 237,120 to 237,863 _0185 PP_0186: PP_0186 - Porphobilinogen deaminase, at 237,982 to 238,923 _0186 Position (kb) 237 238Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 236.269 kb on + strand, within PP_0184at 236.269 kb on + strand, within PP_0184at 236.269 kb on + strand, within PP_0184at 236.270 kb on - strand, within PP_0184at 236.270 kb on - strand, within PP_0184at 236.270 kb on - strand, within PP_0184at 236.495 kb on + strand, within PP_0184at 236.496 kb on - strand, within PP_0184at 236.496 kb on - strand, within PP_0184at 236.717 kb on - strandat 236.867 kb on + strandat 236.867 kb on + strandat 236.868 kb on - strandat 236.868 kb on - strandat 236.868 kb on - strandat 236.868 kb on - strandat 236.868 kb on - strandat 236.971 kb on + strandat 237.076 kb on + strandat 237.076 kb on + strandat 237.076 kb on + strandat 237.077 kb on - strandat 237.081 kb on + strandat 237.081 kb on + strandat 237.081 kb on + strandat 237.082 kb on - strandat 237.082 kb on - strandat 237.082 kb on - strandat 237.083 kb on + strandat 237.083 kb on + strandat 237.083 kb on + strandat 237.083 kb on + strandat 237.084 kb on - strandat 237.084 kb on - strandat 237.084 kb on - strandat 237.084 kb on - strandat 237.084 kb on - strandat 237.119 kb on + strandat 237.119 kb on + strandat 237.120 kb on - strandat 237.195 kb on + strand, within PP_0185at 237.195 kb on + strand, within PP_0185at 237.195 kb on + strand, within PP_0185at 237.196 kb on - strand, within PP_0185at 237.379 kb on - strand, within PP_0185at 237.379 kb on - strand, within PP_0185at 237.379 kb on - strand, within PP_0185at 237.540 kb on - strand, within PP_0185at 237.594 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.615 kb on + strandat 237.615 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.615 kb on + strand, within PP_0185at 237.616 kb on - strand, within PP_0185at 237.616 kb on - strand, within PP_0185at 237.616 kb on - strand, within PP_0185at 237.616 kb on - strand, within PP_0185at 237.616 kb on - strand, within PP_0185at 237.781 kb on - strand, within PP_0185at 237.902 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
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236,269 + PP_0184 0.60 -0.4
236,269 + PP_0184 0.60 -1.9
236,269 + PP_0184 0.60 -1.1
236,270 - PP_0184 0.60 -1.1
236,270 - PP_0184 0.60 -1.2
236,270 - PP_0184 0.60 +2.4
236,495 + PP_0184 0.76 -1.0
236,496 - PP_0184 0.76 +2.4
236,496 - PP_0184 0.76 -1.8
236,717 - +2.4
236,867 + -1.0
236,867 + -0.0
236,868 - +0.2
236,868 - +0.0
236,868 - +1.5
236,868 - +0.2
236,868 - -0.9
236,971 + -0.6
237,076 + +0.1
237,076 + +1.1
237,076 + +0.6
237,077 - -1.0
237,081 + -0.0
237,081 + -0.5
237,081 + +0.5
237,082 - -2.5
237,082 - -0.6
237,082 - +0.8
237,083 + +0.1
237,083 + +0.6
237,083 + -1.0
237,083 + -0.4
237,084 - -0.7
237,084 - -0.9
237,084 - -0.9
237,084 - -1.5
237,084 - -0.9
237,119 + +0.6
237,119 + -0.2
237,120 - -2.0
237,195 + PP_0185 0.10 -1.5
237,195 + PP_0185 0.10 -2.0
237,195 + PP_0185 0.10 -0.4
237,196 - PP_0185 0.10 -1.3
237,379 - PP_0185 0.35 +0.1
237,379 - PP_0185 0.35 -2.1
237,379 - PP_0185 0.35 -2.9
237,540 - PP_0185 0.56 -1.2
237,594 + PP_0185 0.64 +1.5
237,615 + PP_0185 0.67 -2.3
237,615 + PP_0185 0.67 -0.8
237,615 + PP_0185 0.67 -1.2
237,615 + PP_0185 0.67 -1.4
237,615 + PP_0185 0.67 -0.4
237,615 + -0.2
237,615 + PP_0185 0.67 -2.6
237,615 + PP_0185 0.67 -2.0
237,615 + PP_0185 0.67 -0.0
237,616 - PP_0185 0.67 -0.8
237,616 - PP_0185 0.67 -2.6
237,616 - PP_0185 0.67 -1.8
237,616 - PP_0185 0.67 -0.1
237,616 - PP_0185 0.67 -0.2
237,781 - PP_0185 0.89 -3.4
237,902 - +0.0

Or see this region's nucleotide sequence