Strain Fitness in Pseudomonas putida KT2440 around PP_0126

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
131,355	-				+0.8
131,355	-				+0.1
131,356	+				-0.8
131,356	+				+0.8
131,356	+				+2.4
131,357	-				-0.9
131,357	-				+0.3
131,357	-				+1.1
131,357	-				+0.9
131,358	+				+1.1
131,358	+				-0.2
131,358	+				-1.1
131,358	+				+0.8
131,358	+				-0.3
131,358	+				-2.7
131,358	+				-0.2
131,358	+				-0.2
131,358	+				-1.2
131,359	-				+2.8
131,359	-				-0.5
131,359	-				+1.4
131,359	-				+0.2
131,359	-				-1.5
131,359	-				-0.8
131,359	-				-0.3
131,359	-				+0.5
131,359	-				+0.8
131,424	-				-0.4
131,424	-				-1.2
131,424	-				-0.5
131,502	-				-1.4
131,502	-				+0.7
131,502	-				+1.4
131,532	-		PP_0125	0.19	+1.8
131,532	-		PP_0125	0.19	+0.1
131,553	+		PP_0125	0.26	-0.2
131,554	-		PP_0125	0.26	+1.0
131,554	-		PP_0125	0.26	+1.1
131,656	+		PP_0125	0.60	-0.7
131,772	-				-0.6
131,775	+				-1.0
131,775	+				-1.0
131,776	-				-0.8
131,776	-				+0.8
131,776	-				-0.2
131,776	-				+0.2
131,789	+				-0.0
131,789	+				+0.5
131,789	+				+1.6
131,790	-				+1.6
131,803	+				+0.4
131,804	-				-0.1
131,804	-				+0.2
131,804	-				-1.2
131,804	-				+0.1
131,819	+				+2.1
131,819	+				+0.4
131,819	+				-0.4
131,820	-				+0.7
131,820	-				+1.8
131,877	+		PP_0126	0.11	+0.3
131,877	+		PP_0126	0.11	-1.9
131,995	+		PP_0126	0.30	+0.4
132,019	-		PP_0126	0.34	+0.8
132,036	+		PP_0126	0.37	+0.4
132,037	-		PP_0126	0.37	-1.4
132,103	+		PP_0126	0.48	-1.4
132,286	+		PP_0126	0.77	-0.6
132,406	+				-0.8
132,406	+				-1.2
132,407	-				-1.5
132,424	+				+1.1
132,443	+				-0.4
132,443	+				-0.2
132,444	-				-0.3
132,444	-				+0.8
132,444	-				+1.6
132,444	-				-0.2
132,502	-				+1.4
132,502	-				-1.2
132,502	-				+0.7
132,502	-				+1.1
132,572	+				+1.4
132,572	+				-1.2
132,572	+				-0.2
132,573	-				-0.4
132,583	+				-0.2
132,584	-				-1.1
132,584	-				-1.5
132,584	-				+0.8
132,671	+		PP_0127	0.15	-1.9
132,671	+		PP_0127	0.15	+0.4
132,672	-		PP_0127	0.15	+0.4
132,672	-		PP_0127	0.15	-0.8
132,672	-		PP_0127	0.15	-1.5
132,740	+		PP_0127	0.26	-0.8
133,080	-		PP_0127	0.79	-1.5
133,080	-		PP_0127	0.79	-2.2
133,096	-		PP_0127	0.82	-0.9
133,122	-		PP_0127	0.86	+0.1
133,208	+				+0.3
133,208	+				+0.6
133,208	+				+0.4
133,240	+				-0.1
133,240	+				+0.4
133,240	+				+0.8
133,240	+				+1.1
133,240	+				+0.1
133,241	-				+0.3
133,387	+		PP_0128	0.19	+0.8
133,387	+		PP_0128	0.19	+0.7
133,388	-		PP_0128	0.19	+1.1