Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0047 and PP_0048 are separated by 117 nucleotides PP_0048 and PP_5423 are separated by 101 nucleotides PP_5423 and PP_0049 are separated by 189 nucleotides
PP_0047: PP_0047 - response regulator, at 54,227 to 54,901
_0047
PP_0048: PP_0048 - conserved protein of unknown function, at 55,019 to 55,342
_0048
PP_5423: PP_5423 - conserved membrane protein of unknown function, at 55,444 to 55,971
_5423
PP_0049: PP_0049 - conserved protein of unknown function, at 56,161 to 57,627
_0049
Position (kb) 55
56 Strain fitness (log2 ratio) -1
0
1
2 at 54.107 kb on - strand at 54.306 kb on + strand, within PP_0047 at 54.306 kb on + strand, within PP_0047 at 54.307 kb on - strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.381 kb on + strand, within PP_0047 at 54.382 kb on - strand, within PP_0047 at 54.449 kb on - strand, within PP_0047 at 54.600 kb on + strand, within PP_0047 at 54.601 kb on - strand, within PP_0047 at 54.689 kb on + strand, within PP_0047 at 54.699 kb on + strand, within PP_0047 at 54.718 kb on + strand, within PP_0047 at 54.799 kb on - strand, within PP_0047 at 54.831 kb on + strand, within PP_0047 at 54.832 kb on - strand, within PP_0047 at 54.832 kb on - strand, within PP_0047 at 54.862 kb on + strand at 54.863 kb on - strand at 54.900 kb on - strand at 55.016 kb on + strand at 55.017 kb on - strand at 55.025 kb on + strand at 55.025 kb on + strand at 55.027 kb on + strand at 55.028 kb on - strand at 55.028 kb on - strand at 55.214 kb on + strand, within PP_0048 at 55.377 kb on - strand at 55.465 kb on - strand at 55.537 kb on + strand, within PP_5423 at 55.867 kb on - strand, within PP_5423 at 55.877 kb on - strand, within PP_5423 at 55.981 kb on + strand at 55.981 kb on + strand at 55.982 kb on - strand at 55.993 kb on + strand at 56.007 kb on - strand at 56.126 kb on - strand at 56.126 kb on - strand at 56.214 kb on + strand at 56.251 kb on + strand at 56.251 kb on + strand at 56.258 kb on - strand at 56.293 kb on + strand at 56.320 kb on + strand, within PP_0049 at 56.331 kb on + strand, within PP_0049
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 54,107 - -0.6 54,306 + PP_0047 0.12 -1.1 54,306 + PP_0047 0.12 +1.5 54,307 - PP_0047 0.12 -0.6 54,381 + PP_0047 0.23 +0.6 54,381 + PP_0047 0.23 -0.2 54,381 + PP_0047 0.23 -0.1 54,382 - PP_0047 0.23 -0.2 54,449 - PP_0047 0.33 -1.3 54,600 + PP_0047 0.55 -0.6 54,601 - PP_0047 0.55 -0.4 54,689 + PP_0047 0.68 -0.3 54,699 + PP_0047 0.70 +0.3 54,718 + PP_0047 0.73 +1.2 54,799 - PP_0047 0.85 -1.6 54,831 + PP_0047 0.89 +0.2 54,832 - PP_0047 0.90 -0.8 54,832 - PP_0047 0.90 -0.6 54,862 + -0.8 54,863 - +0.1 54,900 - -0.5 55,016 + -0.2 55,017 - -0.8 55,025 + +0.6 55,025 + -0.0 55,027 + +0.1 55,028 - +0.3 55,028 - +0.1 55,214 + PP_0048 0.60 -1.5 55,377 - +0.1 55,465 - +0.6 55,537 + PP_5423 0.18 +0.3 55,867 - PP_5423 0.80 +0.9 55,877 - PP_5423 0.82 +0.3 55,981 + +0.5 55,981 + -0.6 55,982 - +0.1 55,993 + -1.0 56,007 - +0.8 56,126 - -0.3 56,126 - +1.4 56,214 + +1.4 56,251 + -1.0 56,251 + -0.1 56,258 - +2.4 56,293 + +1.2 56,320 + PP_0049 0.11 +0.4 56,331 + PP_0049 0.12 -0.1
Or see this region's nucleotide sequence