Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0021 and PP_0022 are separated by 184 nucleotides PP_0022 and PP_0023 are separated by 445 nucleotides PP_0023 and PP_0024 are separated by 122 nucleotides
PP_0021: PP_0021 - conserved hypothetical protein, at 25,881 to 26,804
_0021
PP_0022: PP_0022 - hypothetical protein, at 26,989 to 27,153
_0022
PP_0023: PP_0023 - conserved hypothetical protein, at 27,599 to 27,727
_0023
PP_0024: PP_0024 - putative membrane-associated metal-dependent hydrolase, at 27,850 to 29,547
_0024
Position (kb) 26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 25.999 kb on + strand, within PP_0021 at 25.999 kb on + strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.000 kb on - strand, within PP_0021 at 26.015 kb on - strand, within PP_0021 at 26.015 kb on - strand, within PP_0021 at 26.056 kb on - strand, within PP_0021 at 26.062 kb on + strand, within PP_0021 at 26.062 kb on + strand, within PP_0021 at 26.063 kb on - strand, within PP_0021 at 26.063 kb on - strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.099 kb on + strand, within PP_0021 at 26.100 kb on - strand, within PP_0021 at 26.100 kb on - strand, within PP_0021 at 26.100 kb on - strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.102 kb on + strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.103 kb on - strand, within PP_0021 at 26.132 kb on + strand, within PP_0021 at 26.132 kb on + strand, within PP_0021 at 26.158 kb on - strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.233 kb on + strand, within PP_0021 at 26.234 kb on - strand, within PP_0021 at 26.234 kb on - strand, within PP_0021 at 26.262 kb on + strand, within PP_0021 at 26.263 kb on - strand, within PP_0021 at 26.328 kb on + strand, within PP_0021 at 26.328 kb on + strand, within PP_0021 at 26.328 kb on + strand, within PP_0021 at 26.346 kb on + strand, within PP_0021 at 26.346 kb on + strand, within PP_0021 at 26.352 kb on + strand, within PP_0021 at 26.352 kb on + strand, within PP_0021 at 26.352 kb on + strand, within PP_0021 at 26.353 kb on - strand, within PP_0021 at 26.353 kb on - strand, within PP_0021 at 26.355 kb on - strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.394 kb on + strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.395 kb on - strand, within PP_0021 at 26.459 kb on + strand, within PP_0021 at 26.459 kb on + strand, within PP_0021 at 26.459 kb on + strand, within PP_0021 at 26.460 kb on - strand, within PP_0021 at 26.517 kb on + strand, within PP_0021 at 26.542 kb on - strand, within PP_0021 at 26.626 kb on + strand, within PP_0021 at 26.626 kb on + strand, within PP_0021 at 26.627 kb on - strand, within PP_0021 at 26.627 kb on - strand, within PP_0021 at 26.627 kb on - strand, within PP_0021 at 26.733 kb on + strand at 26.734 kb on - strand at 26.747 kb on + strand at 26.747 kb on + strand at 26.818 kb on - strand at 26.837 kb on + strand at 26.838 kb on - strand at 26.851 kb on + strand at 26.851 kb on + strand at 26.851 kb on + strand at 26.852 kb on - strand at 26.871 kb on + strand at 26.878 kb on + strand at 26.879 kb on - strand at 26.879 kb on - strand at 26.882 kb on + strand at 26.882 kb on + strand at 26.883 kb on - strand at 26.955 kb on + strand at 26.955 kb on + strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.956 kb on - strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.990 kb on + strand at 26.991 kb on - strand at 26.992 kb on + strand at 26.993 kb on - strand at 26.993 kb on - strand at 26.993 kb on - strand at 27.091 kb on - strand, within PP_0022 at 27.140 kb on + strand at 27.140 kb on + strand at 27.141 kb on - strand at 27.173 kb on - strand at 27.211 kb on - strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.291 kb on + strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.292 kb on - strand at 27.321 kb on + strand at 27.322 kb on - strand at 27.322 kb on - strand at 27.322 kb on - strand at 27.432 kb on + strand at 27.433 kb on - strand at 27.462 kb on - strand at 27.476 kb on + strand at 27.476 kb on + strand at 27.600 kb on + strand at 27.601 kb on - strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.766 kb on + strand at 27.767 kb on - strand at 27.767 kb on - strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.770 kb on + strand at 27.771 kb on - strand at 27.771 kb on - strand at 27.774 kb on + strand at 27.775 kb on - strand at 27.775 kb on - strand at 27.863 kb on + strand at 27.863 kb on + strand at 27.864 kb on - strand at 27.864 kb on - strand at 27.864 kb on - strand at 27.885 kb on + strand at 27.885 kb on + strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.886 kb on - strand at 27.917 kb on + strand at 28.054 kb on + strand, within PP_0024 at 28.055 kb on - strand, within PP_0024 at 28.055 kb on - strand, within PP_0024 at 28.094 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.108 kb on + strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.109 kb on - strand, within PP_0024 at 28.112 kb on + strand, within PP_0024 at 28.113 kb on - strand, within PP_0024 at 28.113 kb on - strand, within PP_0024
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 25,999 + PP_0021 0.13 -1.2 25,999 + PP_0021 0.13 +0.1 26,000 - PP_0021 0.13 +0.3 26,000 - PP_0021 0.13 +0.3 26,000 - PP_0021 0.13 +1.1 26,000 - PP_0021 0.13 -0.1 26,000 - PP_0021 0.13 +0.5 26,000 - PP_0021 0.13 +0.1 26,000 - PP_0021 0.13 -0.3 26,015 - PP_0021 0.15 -0.9 26,015 - PP_0021 0.15 +2.4 26,056 - PP_0021 0.19 +0.3 26,062 + PP_0021 0.20 +1.4 26,062 + PP_0021 0.20 -0.3 26,063 - PP_0021 0.20 +1.4 26,063 - PP_0021 0.20 -0.5 26,099 + PP_0021 0.24 -1.9 26,099 + PP_0021 0.24 +0.4 26,099 + PP_0021 0.24 +0.3 26,099 + PP_0021 0.24 -0.1 26,099 + PP_0021 0.24 -0.2 26,100 - PP_0021 0.24 +0.4 26,100 - PP_0021 0.24 +0.0 26,100 - PP_0021 0.24 +0.2 26,102 + PP_0021 0.24 -1.0 26,102 + PP_0021 0.24 -0.2 26,102 + PP_0021 0.24 -0.5 26,102 + PP_0021 0.24 -0.6 26,103 - PP_0021 0.24 +0.9 26,103 - PP_0021 0.24 +0.1 26,103 - PP_0021 0.24 -0.8 26,103 - PP_0021 0.24 +0.8 26,103 - PP_0021 0.24 -0.2 26,132 + PP_0021 0.27 +0.1 26,132 + PP_0021 0.27 +0.0 26,158 - PP_0021 0.30 -0.9 26,233 + PP_0021 0.38 -0.9 26,233 + PP_0021 0.38 -0.2 26,233 + PP_0021 0.38 -0.2 26,233 + PP_0021 0.38 +2.4 26,234 - PP_0021 0.38 -0.1 26,234 - PP_0021 0.38 +0.8 26,262 + PP_0021 0.41 +0.2 26,263 - PP_0021 0.41 +1.4 26,328 + PP_0021 0.48 -0.8 26,328 + PP_0021 0.48 -1.8 26,328 + PP_0021 0.48 +1.4 26,346 + PP_0021 0.50 -0.7 26,346 + PP_0021 0.50 -0.5 26,352 + PP_0021 0.51 -0.3 26,352 + PP_0021 0.51 +1.1 26,352 + PP_0021 0.51 +0.2 26,353 - PP_0021 0.51 +0.1 26,353 - PP_0021 0.51 -0.2 26,355 - PP_0021 0.51 -0.4 26,394 + PP_0021 0.56 +0.1 26,394 + PP_0021 0.56 +1.1 26,394 + PP_0021 0.56 +1.5 26,394 + PP_0021 0.56 -0.1 26,394 + PP_0021 0.56 +1.3 26,394 + PP_0021 0.56 -0.5 26,394 + PP_0021 0.56 -0.4 26,394 + PP_0021 0.56 -0.3 26,394 + PP_0021 0.56 +0.5 26,394 + PP_0021 0.56 -0.7 26,394 + PP_0021 0.56 +0.3 26,394 + PP_0021 0.56 +0.5 26,395 - PP_0021 0.56 -0.3 26,395 - PP_0021 0.56 +0.1 26,395 - PP_0021 0.56 +0.7 26,395 - +0.8 26,395 - PP_0021 0.56 +0.4 26,395 - PP_0021 0.56 +0.1 26,395 - PP_0021 0.56 +1.1 26,395 - PP_0021 0.56 -0.2 26,395 - PP_0021 0.56 -0.4 26,459 + PP_0021 0.63 +0.1 26,459 + PP_0021 0.63 +1.4 26,459 + PP_0021 0.63 -0.8 26,460 - PP_0021 0.63 +0.3 26,517 + PP_0021 0.69 -0.3 26,542 - PP_0021 0.72 -0.9 26,626 + PP_0021 0.81 -2.8 26,626 + PP_0021 0.81 -0.9 26,627 - PP_0021 0.81 -0.0 26,627 - PP_0021 0.81 -1.5 26,627 - PP_0021 0.81 +0.3 26,733 + -0.4 26,734 - +0.8 26,747 + +1.4 26,747 + -0.5 26,818 - +0.2 26,837 + +0.4 26,838 - +0.1 26,851 + +0.6 26,851 + -1.0 26,851 + +0.6 26,852 - -0.7 26,871 + +3.4 26,878 + -0.8 26,879 - -0.7 26,879 - -0.0 26,882 + -2.5 26,882 + +2.0 26,883 - +2.4 26,955 + +1.1 26,955 + +0.0 26,956 - +0.7 26,956 - +1.4 26,956 - -0.2 26,956 - -1.8 26,956 - -1.4 26,956 - +0.3 26,956 - -0.2 26,990 + -0.7 26,990 + +0.2 26,990 + +3.4 26,990 + -0.4 26,991 - -1.0 26,992 + +0.1 26,993 - +0.1 26,993 - -1.2 26,993 - -1.0 27,091 - PP_0022 0.62 -0.7 27,140 + -0.5 27,140 + -0.3 27,141 - -0.6 27,173 - -0.9 27,211 - -0.4 27,291 + -2.3 27,291 + +1.3 27,291 + +0.4 27,291 + -1.2 27,291 + +0.3 27,291 + -0.1 27,291 + -0.2 27,291 + -1.2 27,291 + -0.4 27,292 - -0.2 27,292 - +0.9 27,292 - +0.4 27,292 - -1.4 27,292 - +0.0 27,292 - -0.5 27,292 - -0.9 27,292 - -0.3 27,292 - -1.5 27,292 - -0.5 27,292 - +1.8 27,292 - +0.1 27,292 - +0.3 27,292 - -1.4 27,292 - +0.4 27,321 + +0.6 27,322 - -0.4 27,322 - -0.2 27,322 - -0.1 27,432 + +0.8 27,433 - -0.2 27,462 - +0.4 27,476 + -0.9 27,476 + +0.3 27,600 + +0.6 27,601 - -1.3 27,766 + -0.4 27,766 + -0.4 27,766 + -0.2 27,766 + -0.2 27,767 - -2.9 27,767 - -1.4 27,770 + -0.2 27,770 + +0.5 27,770 + +0.5 27,770 + -0.5 27,770 + +0.9 27,771 - -0.0 27,771 - +0.2 27,774 + -1.0 27,775 - -0.3 27,775 - -1.3 27,863 + -0.9 27,863 + -1.2 27,864 - +2.4 27,864 - -0.7 27,864 - -0.1 27,885 + -2.2 27,885 + -0.3 27,886 - +0.9 27,886 - -1.5 27,886 - +0.3 27,886 - +0.9 27,886 - -0.1 27,917 + +0.1 28,054 + PP_0024 0.12 +0.3 28,055 - PP_0024 0.12 +0.4 28,055 - PP_0024 0.12 +0.7 28,094 + PP_0024 0.14 -0.6 28,108 + PP_0024 0.15 +0.1 28,108 + PP_0024 0.15 -0.2 28,108 + PP_0024 0.15 -0.7 28,108 + PP_0024 0.15 +1.1 28,109 - PP_0024 0.15 +0.1 28,109 - PP_0024 0.15 -0.9 28,109 - PP_0024 0.15 +0.0 28,112 + PP_0024 0.15 +0.0 28,113 - PP_0024 0.15 -1.4 28,113 - PP_0024 0.15 -1.1
Or see this region's nucleotide sequence