Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3257 and Atu3258 are separated by 27 nucleotides Atu3258 and Atu3259 are separated by 174 nucleotides Atu3259 and Atu3260 are separated by 53 nucleotides
Atu3257: Atu3257 - mannonate dehydratase, at 273,985 to 275,193
Atu3257
Atu3258: Atu3258 - hypothetical protein, at 275,221 to 275,643
Atu3258
Atu3259: Atu3259 - dehydrogenase, at 275,818 to 277,329
Atu3259
Atu3260: Atu3260 - ABC transporter substrate binding protein, at 277,383 to 278,381
Atu3260
Position (kb) 275
276
277
278 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 274.884 kb on - strand, within Atu3257 at 274.990 kb on - strand, within Atu3257 at 274.990 kb on - strand, within Atu3257 at 275.037 kb on - strand, within Atu3257 at 275.086 kb on + strand at 275.111 kb on + strand at 275.182 kb on + strand at 275.252 kb on + strand at 275.316 kb on + strand, within Atu3258 at 275.319 kb on - strand, within Atu3258 at 275.487 kb on + strand, within Atu3258 at 275.487 kb on + strand, within Atu3258 at 275.577 kb on + strand, within Atu3258 at 275.694 kb on + strand at 275.694 kb on + strand at 275.694 kb on + strand at 275.702 kb on - strand at 275.702 kb on - strand at 275.702 kb on - strand at 275.729 kb on - strand at 275.996 kb on + strand, within Atu3259 at 276.105 kb on + strand, within Atu3259 at 276.145 kb on - strand, within Atu3259 at 276.459 kb on + strand, within Atu3259 at 276.467 kb on - strand, within Atu3259 at 276.510 kb on + strand, within Atu3259 at 276.510 kb on + strand, within Atu3259 at 276.518 kb on - strand, within Atu3259 at 276.518 kb on - strand, within Atu3259 at 276.571 kb on + strand, within Atu3259 at 276.606 kb on + strand, within Atu3259 at 276.675 kb on + strand, within Atu3259 at 276.675 kb on + strand, within Atu3259 at 276.677 kb on - strand, within Atu3259 at 276.683 kb on - strand, within Atu3259 at 276.683 kb on - strand, within Atu3259 at 276.706 kb on + strand, within Atu3259 at 276.706 kb on + strand, within Atu3259 at 276.808 kb on - strand, within Atu3259 at 276.899 kb on + strand, within Atu3259 at 276.907 kb on - strand, within Atu3259 at 276.914 kb on + strand, within Atu3259 at 276.922 kb on - strand, within Atu3259 at 276.984 kb on - strand, within Atu3259 at 277.002 kb on + strand, within Atu3259 at 277.006 kb on - strand, within Atu3259 at 277.012 kb on + strand, within Atu3259 at 277.142 kb on + strand, within Atu3259 at 277.172 kb on + strand, within Atu3259 at 277.180 kb on - strand at 277.180 kb on - strand at 277.205 kb on + strand at 277.388 kb on - strand at 277.394 kb on + strand at 277.407 kb on - strand at 277.459 kb on + strand at 277.467 kb on - strand at 277.467 kb on - strand at 277.467 kb on - strand at 277.504 kb on + strand, within Atu3260 at 277.504 kb on + strand, within Atu3260 at 277.504 kb on + strand, within Atu3260 at 277.612 kb on + strand, within Atu3260 at 277.744 kb on + strand, within Atu3260 at 277.783 kb on - strand, within Atu3260 at 277.805 kb on - strand, within Atu3260 at 277.810 kb on - strand, within Atu3260 at 277.842 kb on + strand, within Atu3260 at 277.877 kb on + strand, within Atu3260 at 278.012 kb on + strand, within Atu3260 at 278.078 kb on - strand, within Atu3260 at 278.097 kb on - strand, within Atu3260 at 278.108 kb on - strand, within Atu3260 at 278.108 kb on - strand, within Atu3260 at 278.108 kb on - strand, within Atu3260 at 278.108 kb on - strand, within Atu3260 at 278.118 kb on - strand, within Atu3260 at 278.138 kb on + strand, within Atu3260 at 278.163 kb on - strand, within Atu3260 at 278.284 kb on + strand at 278.292 kb on - strand at 278.309 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 274,884 - Atu3257 0.74 -0.2 274,990 - Atu3257 0.83 +1.0 274,990 - Atu3257 0.83 -0.1 275,037 - Atu3257 0.87 -0.4 275,086 + +0.1 275,111 + +0.7 275,182 + +0.2 275,252 + +0.1 275,316 + Atu3258 0.22 +0.5 275,319 - Atu3258 0.23 -0.3 275,487 + Atu3258 0.63 +2.0 275,487 + Atu3258 0.63 +0.1 275,577 + Atu3258 0.84 -2.2 275,694 + -0.8 275,694 + -0.3 275,694 + -1.0 275,702 - -0.3 275,702 - -1.1 275,702 - +4.6 275,729 - -0.2 275,996 + Atu3259 0.12 +1.2 276,105 + Atu3259 0.19 +0.1 276,145 - Atu3259 0.22 -0.3 276,459 + Atu3259 0.42 -1.0 276,467 - Atu3259 0.43 +1.0 276,510 + Atu3259 0.46 +0.2 276,510 + Atu3259 0.46 +0.4 276,518 - Atu3259 0.46 +0.1 276,518 - Atu3259 0.46 +0.0 276,571 + Atu3259 0.50 +1.1 276,606 + Atu3259 0.52 -0.3 276,675 + Atu3259 0.57 +0.0 276,675 + Atu3259 0.57 +0.7 276,677 - Atu3259 0.57 -3.8 276,683 - Atu3259 0.57 +0.1 276,683 - Atu3259 0.57 +0.1 276,706 + Atu3259 0.59 +0.4 276,706 + Atu3259 0.59 +1.4 276,808 - Atu3259 0.65 -0.2 276,899 + Atu3259 0.71 -0.0 276,907 - Atu3259 0.72 +0.8 276,914 + Atu3259 0.72 -1.6 276,922 - Atu3259 0.73 -1.5 276,984 - Atu3259 0.77 +0.2 277,002 + Atu3259 0.78 +0.5 277,006 - Atu3259 0.79 -0.1 277,012 + Atu3259 0.79 +0.0 277,142 + Atu3259 0.88 -1.6 277,172 + Atu3259 0.90 +2.1 277,180 - -0.4 277,180 - +1.7 277,205 + +0.1 277,388 - +0.9 277,394 + -0.2 277,407 - -0.3 277,459 + -1.7 277,467 - -1.4 277,467 - +0.4 277,467 - +0.6 277,504 + Atu3260 0.12 -0.1 277,504 + Atu3260 0.12 +0.1 277,504 + Atu3260 0.12 +2.5 277,612 + Atu3260 0.23 -0.7 277,744 + Atu3260 0.36 +1.0 277,783 - Atu3260 0.40 -0.1 277,805 - Atu3260 0.42 +0.2 277,810 - Atu3260 0.43 +0.1 277,842 + Atu3260 0.46 +0.1 277,877 + Atu3260 0.49 -0.1 278,012 + Atu3260 0.63 +4.1 278,078 - Atu3260 0.70 -0.6 278,097 - Atu3260 0.71 -0.4 278,108 - Atu3260 0.73 +0.4 278,108 - Atu3260 0.73 +0.3 278,108 - Atu3260 0.73 +0.3 278,108 - Atu3260 0.73 -0.1 278,118 - Atu3260 0.74 +0.8 278,138 + Atu3260 0.76 -1.5 278,163 - Atu3260 0.78 -1.5 278,284 + -0.1 278,292 - -1.9 278,309 + -0.3
Or see this region's nucleotide sequence