Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5220 and PP_5221 are separated by 203 nucleotides PP_5221 and PP_5222 are separated by 130 nucleotides PP_5222 and PP_5223 are separated by 69 nucleotides PP_5223 and PP_5224 are separated by 146 nucleotides PP_5224 and PP_5225 are separated by 1 nucleotides
PP_5220: PP_5220 - putative hydrolase, at 5,955,109 to 5,955,774
_5220
PP_5221: PP_5221 - conserved protein of unknown function, UPF0178 family, at 5,955,978 to 5,956,466
_5221
PP_5222: PP_5222 - adenylate cyclase, at 5,956,597 to 5,959,452
_5222
PP_5223: PP_5223 - regulator of nucleotide diphosphokinase, at 5,959,522 to 5,959,932
_5223
PP_5224: PP_5224 - conserved protein of unknown function, at 5,960,079 to 5,960,303
_5224
PP_5225: PP_5225 - frataxin, at 5,960,305 to 5,960,637
_5225
Position (kb) 5956
5957
5958
5959
5960 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5955.642 kb on - strand, within PP_5220 at 5955.642 kb on - strand, within PP_5220 at 5955.642 kb on - strand, within PP_5220 at 5955.691 kb on + strand, within PP_5220 at 5955.691 kb on + strand, within PP_5220 at 5955.692 kb on - strand, within PP_5220 at 5955.733 kb on + strand at 5955.733 kb on + strand at 5955.772 kb on + strand at 5955.772 kb on + strand at 5955.777 kb on + strand at 5955.778 kb on - strand at 5955.778 kb on - strand at 5955.778 kb on - strand at 5955.778 kb on - strand at 5955.898 kb on - strand at 5956.048 kb on + strand, within PP_5221 at 5956.252 kb on + strand, within PP_5221 at 5956.252 kb on + strand, within PP_5221 at 5956.253 kb on - strand, within PP_5221 at 5956.422 kb on + strand at 5956.422 kb on + strand at 5956.423 kb on - strand at 5956.423 kb on - strand at 5956.423 kb on - strand at 5956.442 kb on + strand at 5956.442 kb on + strand at 5956.442 kb on + strand at 5956.442 kb on + strand at 5956.443 kb on - strand at 5956.443 kb on - strand at 5956.443 kb on - strand at 5956.529 kb on + strand at 5956.529 kb on + strand at 5956.529 kb on + strand at 5956.742 kb on + strand at 5956.742 kb on + strand at 5956.742 kb on + strand at 5956.742 kb on + strand at 5956.742 kb on + strand at 5956.743 kb on - strand at 5956.795 kb on + strand at 5956.824 kb on - strand at 5956.832 kb on - strand at 5956.888 kb on + strand, within PP_5222 at 5956.888 kb on + strand, within PP_5222 at 5956.888 kb on + strand, within PP_5222 at 5956.889 kb on - strand, within PP_5222 at 5956.889 kb on - strand, within PP_5222 at 5956.889 kb on - strand, within PP_5222 at 5956.889 kb on - strand, within PP_5222 at 5956.889 kb on - strand, within PP_5222 at 5957.179 kb on + strand, within PP_5222 at 5957.250 kb on - strand at 5957.257 kb on + strand, within PP_5222 at 5957.258 kb on - strand, within PP_5222 at 5957.273 kb on - strand, within PP_5222 at 5957.273 kb on - strand, within PP_5222 at 5957.273 kb on - strand, within PP_5222 at 5957.275 kb on + strand, within PP_5222 at 5957.275 kb on + strand, within PP_5222 at 5957.275 kb on + strand, within PP_5222 at 5957.276 kb on - strand, within PP_5222 at 5957.276 kb on - strand, within PP_5222 at 5957.276 kb on - strand, within PP_5222 at 5957.281 kb on + strand, within PP_5222 at 5957.281 kb on + strand, within PP_5222 at 5957.281 kb on + strand, within PP_5222 at 5957.281 kb on + strand, within PP_5222 at 5957.281 kb on + strand, within PP_5222 at 5957.282 kb on - strand, within PP_5222 at 5957.282 kb on - strand, within PP_5222 at 5957.282 kb on - strand, within PP_5222 at 5957.452 kb on + strand, within PP_5222 at 5957.453 kb on - strand, within PP_5222 at 5957.494 kb on + strand, within PP_5222 at 5957.495 kb on - strand, within PP_5222 at 5957.495 kb on - strand, within PP_5222 at 5957.545 kb on + strand, within PP_5222 at 5957.546 kb on - strand, within PP_5222 at 5957.546 kb on - strand, within PP_5222 at 5957.546 kb on - strand, within PP_5222 at 5957.575 kb on + strand, within PP_5222 at 5957.575 kb on + strand, within PP_5222 at 5957.575 kb on + strand, within PP_5222 at 5957.615 kb on + strand, within PP_5222 at 5957.616 kb on - strand, within PP_5222 at 5957.616 kb on - strand, within PP_5222 at 5957.616 kb on - strand, within PP_5222 at 5957.630 kb on - strand, within PP_5222 at 5957.812 kb on - strand, within PP_5222 at 5957.822 kb on - strand, within PP_5222 at 5957.909 kb on - strand, within PP_5222 at 5958.029 kb on - strand, within PP_5222 at 5958.029 kb on - strand, within PP_5222 at 5958.029 kb on - strand, within PP_5222 at 5958.072 kb on - strand, within PP_5222 at 5958.072 kb on - strand, within PP_5222 at 5958.108 kb on - strand, within PP_5222 at 5958.237 kb on + strand, within PP_5222 at 5958.238 kb on - strand, within PP_5222 at 5958.238 kb on - strand, within PP_5222 at 5958.394 kb on + strand, within PP_5222 at 5958.394 kb on + strand, within PP_5222 at 5958.394 kb on + strand, within PP_5222 at 5958.395 kb on - strand, within PP_5222 at 5958.395 kb on - strand, within PP_5222 at 5958.475 kb on + strand, within PP_5222 at 5958.476 kb on - strand, within PP_5222 at 5958.476 kb on - strand, within PP_5222 at 5958.476 kb on - strand, within PP_5222 at 5958.560 kb on + strand, within PP_5222 at 5958.605 kb on + strand, within PP_5222 at 5958.661 kb on + strand, within PP_5222 at 5958.661 kb on + strand, within PP_5222 at 5958.668 kb on + strand, within PP_5222 at 5958.668 kb on + strand, within PP_5222 at 5958.668 kb on + strand, within PP_5222 at 5958.668 kb on + strand, within PP_5222 at 5958.668 kb on + strand, within PP_5222 at 5958.669 kb on - strand, within PP_5222 at 5958.758 kb on - strand, within PP_5222 at 5958.781 kb on + strand, within PP_5222 at 5958.793 kb on + strand, within PP_5222 at 5958.793 kb on + strand, within PP_5222 at 5958.861 kb on + strand, within PP_5222 at 5958.861 kb on + strand, within PP_5222 at 5958.875 kb on - strand at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.904 kb on + strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.905 kb on - strand, within PP_5222 at 5958.919 kb on + strand, within PP_5222 at 5958.919 kb on + strand, within PP_5222 at 5958.919 kb on + strand, within PP_5222 at 5958.919 kb on + strand, within PP_5222 at 5958.919 kb on + strand, within PP_5222 at 5958.920 kb on - strand, within PP_5222 at 5958.920 kb on - strand, within PP_5222 at 5958.920 kb on - strand, within PP_5222 at 5959.150 kb on + strand, within PP_5222 at 5959.150 kb on + strand, within PP_5222 at 5959.150 kb on + strand, within PP_5222 at 5959.150 kb on + strand, within PP_5222 at 5959.150 kb on + strand, within PP_5222 at 5959.150 kb on + strand, within PP_5222 at 5959.151 kb on - strand, within PP_5222 at 5959.151 kb on - strand, within PP_5222 at 5959.151 kb on - strand, within PP_5222 at 5959.151 kb on - strand, within PP_5222 at 5959.151 kb on - strand, within PP_5222 at 5959.153 kb on + strand, within PP_5222 at 5959.153 kb on + strand, within PP_5222 at 5959.153 kb on + strand, within PP_5222 at 5959.153 kb on + strand, within PP_5222 at 5959.154 kb on - strand, within PP_5222 at 5959.154 kb on - strand, within PP_5222 at 5959.154 kb on - strand, within PP_5222 at 5959.210 kb on + strand at 5959.210 kb on + strand at 5959.258 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.318 kb on + strand at 5959.319 kb on - strand at 5959.319 kb on - strand at 5959.319 kb on - strand at 5959.319 kb on - strand at 5959.319 kb on - strand at 5959.319 kb on - strand at 5959.319 kb on - strand at 5959.320 kb on + strand at 5959.321 kb on - strand at 5959.379 kb on - strand at 5959.379 kb on - strand at 5959.387 kb on + strand at 5959.387 kb on + strand at 5959.388 kb on - strand at 5959.388 kb on - strand at 5959.523 kb on + strand at 5959.523 kb on + strand at 5959.523 kb on + strand at 5959.524 kb on - strand at 5959.532 kb on + strand at 5959.533 kb on - strand at 5959.556 kb on + strand at 5959.556 kb on + strand at 5959.557 kb on - strand at 5959.557 kb on - strand at 5959.718 kb on + strand, within PP_5223 at 5959.718 kb on + strand, within PP_5223 at 5959.719 kb on - strand, within PP_5223 at 5959.851 kb on + strand, within PP_5223 at 5959.891 kb on + strand, within PP_5223 at 5959.891 kb on + strand, within PP_5223 at 5959.891 kb on + strand, within PP_5223 at 5959.891 kb on + strand, within PP_5223 at 5959.892 kb on - strand at 5959.892 kb on - strand at 5959.892 kb on - strand at 5960.045 kb on + strand at 5960.045 kb on + strand at 5960.046 kb on - strand at 5960.062 kb on + strand at 5960.223 kb on + strand, within PP_5224 at 5960.224 kb on - strand, within PP_5224 at 5960.224 kb on - strand, within PP_5224 at 5960.224 kb on - strand, within PP_5224 at 5960.266 kb on + strand, within PP_5224 at 5960.267 kb on - strand, within PP_5224 at 5960.298 kb on - strand at 5960.353 kb on - strand, within PP_5225
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 5,955,642 - PP_5220 0.80 +0.5 5,955,642 - PP_5220 0.80 +0.1 5,955,642 - PP_5220 0.80 -0.7 5,955,691 + PP_5220 0.87 +2.0 5,955,691 + PP_5220 0.87 -0.3 5,955,692 - PP_5220 0.88 +0.2 5,955,733 + -0.9 5,955,733 + +0.0 5,955,772 + -0.8 5,955,772 + -0.1 5,955,777 + +1.7 5,955,778 - -0.6 5,955,778 - -0.7 5,955,778 - +0.7 5,955,778 - -0.5 5,955,898 - -1.0 5,956,048 + PP_5221 0.14 -0.1 5,956,252 + PP_5221 0.56 -0.2 5,956,252 + PP_5221 0.56 +0.1 5,956,253 - PP_5221 0.56 -0.1 5,956,422 + -0.8 5,956,422 + -0.2 5,956,423 - +0.7 5,956,423 - -0.4 5,956,423 - +0.9 5,956,442 + +0.3 5,956,442 + +0.2 5,956,442 + -0.4 5,956,442 + -0.6 5,956,443 - -0.1 5,956,443 - +0.4 5,956,443 - +0.5 5,956,529 + +1.0 5,956,529 + -0.5 5,956,529 + -0.6 5,956,742 + +0.1 5,956,742 + +0.6 5,956,742 + -1.7 5,956,742 + +0.6 5,956,742 + +0.3 5,956,743 - -0.8 5,956,795 + -0.1 5,956,824 - -1.2 5,956,832 - +1.5 5,956,888 + PP_5222 0.10 +0.0 5,956,888 + PP_5222 0.10 -0.1 5,956,888 + PP_5222 0.10 +0.8 5,956,889 - PP_5222 0.10 -0.9 5,956,889 - PP_5222 0.10 -0.4 5,956,889 - PP_5222 0.10 -0.2 5,956,889 - PP_5222 0.10 -0.0 5,956,889 - PP_5222 0.10 -0.9 5,957,179 + PP_5222 0.20 +1.0 5,957,250 - +1.6 5,957,257 + PP_5222 0.23 +0.1 5,957,258 - PP_5222 0.23 -0.8 5,957,273 - PP_5222 0.24 +1.0 5,957,273 - PP_5222 0.24 +0.2 5,957,273 - PP_5222 0.24 +0.1 5,957,275 + PP_5222 0.24 +0.6 5,957,275 + PP_5222 0.24 -1.8 5,957,275 + PP_5222 0.24 +0.7 5,957,276 - PP_5222 0.24 -1.5 5,957,276 - PP_5222 0.24 +0.6 5,957,276 - PP_5222 0.24 +0.5 5,957,281 + PP_5222 0.24 +1.0 5,957,281 + PP_5222 0.24 +0.9 5,957,281 + PP_5222 0.24 -0.4 5,957,281 + PP_5222 0.24 -0.0 5,957,281 + PP_5222 0.24 +1.0 5,957,282 - PP_5222 0.24 -0.5 5,957,282 - PP_5222 0.24 +0.9 5,957,282 - PP_5222 0.24 -0.3 5,957,452 + PP_5222 0.30 -0.6 5,957,453 - PP_5222 0.30 -1.9 5,957,494 + PP_5222 0.31 +1.2 5,957,495 - PP_5222 0.31 +0.8 5,957,495 - PP_5222 0.31 +0.2 5,957,545 + PP_5222 0.33 +2.0 5,957,546 - PP_5222 0.33 +1.6 5,957,546 - PP_5222 0.33 -0.2 5,957,546 - PP_5222 0.33 +0.1 5,957,575 + PP_5222 0.34 +1.3 5,957,575 + PP_5222 0.34 +0.8 5,957,575 + PP_5222 0.34 -0.2 5,957,615 + PP_5222 0.36 +1.9 5,957,616 - PP_5222 0.36 +0.2 5,957,616 - PP_5222 0.36 +0.3 5,957,616 - PP_5222 0.36 -1.4 5,957,630 - PP_5222 0.36 -0.7 5,957,812 - PP_5222 0.43 -0.6 5,957,822 - PP_5222 0.43 -0.0 5,957,909 - PP_5222 0.46 +1.5 5,958,029 - PP_5222 0.50 -1.0 5,958,029 - PP_5222 0.50 +0.8 5,958,029 - PP_5222 0.50 -0.3 5,958,072 - PP_5222 0.52 +0.0 5,958,072 - PP_5222 0.52 -0.2 5,958,108 - PP_5222 0.53 -2.5 5,958,237 + PP_5222 0.57 -0.4 5,958,238 - PP_5222 0.57 -0.2 5,958,238 - PP_5222 0.57 -0.7 5,958,394 + PP_5222 0.63 -0.1 5,958,394 + PP_5222 0.63 -1.0 5,958,394 + PP_5222 0.63 +0.0 5,958,395 - PP_5222 0.63 -0.8 5,958,395 - PP_5222 0.63 -0.1 5,958,475 + PP_5222 0.66 +1.5 5,958,476 - PP_5222 0.66 -0.0 5,958,476 - PP_5222 0.66 +0.4 5,958,476 - PP_5222 0.66 +0.3 5,958,560 + PP_5222 0.69 -0.1 5,958,605 + PP_5222 0.70 -0.8 5,958,661 + PP_5222 0.72 -0.3 5,958,661 + PP_5222 0.72 +0.3 5,958,668 + PP_5222 0.73 -0.4 5,958,668 + PP_5222 0.73 +0.6 5,958,668 + PP_5222 0.73 +0.4 5,958,668 + PP_5222 0.73 -0.2 5,958,668 + PP_5222 0.73 +0.7 5,958,669 - PP_5222 0.73 -0.9 5,958,758 - PP_5222 0.76 -1.5 5,958,781 + PP_5222 0.76 -1.0 5,958,793 + PP_5222 0.77 +0.5 5,958,793 + PP_5222 0.77 -1.2 5,958,861 + PP_5222 0.79 +0.8 5,958,861 + PP_5222 0.79 +0.1 5,958,875 - -0.5 5,958,904 + PP_5222 0.81 -0.4 5,958,904 + PP_5222 0.81 -0.3 5,958,904 + PP_5222 0.81 +0.3 5,958,904 + PP_5222 0.81 +2.0 5,958,904 + PP_5222 0.81 +0.5 5,958,904 + PP_5222 0.81 +0.8 5,958,904 + PP_5222 0.81 -0.1 5,958,904 + PP_5222 0.81 +0.3 5,958,905 - PP_5222 0.81 +3.0 5,958,905 - PP_5222 0.81 +0.2 5,958,905 - PP_5222 0.81 +0.5 5,958,905 - PP_5222 0.81 +0.8 5,958,905 - PP_5222 0.81 +1.5 5,958,905 - PP_5222 0.81 -0.2 5,958,905 - PP_5222 0.81 -1.1 5,958,919 + PP_5222 0.81 +1.0 5,958,919 + PP_5222 0.81 +0.5 5,958,919 + PP_5222 0.81 +0.1 5,958,919 + PP_5222 0.81 +0.5 5,958,919 + PP_5222 0.81 +0.5 5,958,920 - PP_5222 0.81 +0.4 5,958,920 - PP_5222 0.81 +0.8 5,958,920 - PP_5222 0.81 -0.5 5,959,150 + PP_5222 0.89 +1.0 5,959,150 + PP_5222 0.89 +0.9 5,959,150 + PP_5222 0.89 -0.5 5,959,150 + PP_5222 0.89 -0.2 5,959,150 + PP_5222 0.89 +3.0 5,959,150 + PP_5222 0.89 -1.7 5,959,151 - PP_5222 0.89 +0.5 5,959,151 - PP_5222 0.89 +0.8 5,959,151 - PP_5222 0.89 -0.4 5,959,151 - PP_5222 0.89 +0.0 5,959,151 - PP_5222 0.89 +0.0 5,959,153 + PP_5222 0.89 +0.1 5,959,153 + PP_5222 0.89 +0.6 5,959,153 + PP_5222 0.89 -0.3 5,959,153 + PP_5222 0.89 +0.7 5,959,154 - PP_5222 0.90 +1.2 5,959,154 - PP_5222 0.90 -0.1 5,959,154 - PP_5222 0.90 +1.7 5,959,210 + -0.0 5,959,210 + +2.0 5,959,258 + +0.5 5,959,318 + -0.2 5,959,318 + -1.5 5,959,318 + +0.2 5,959,318 + +0.4 5,959,318 + +0.7 5,959,318 + +1.0 5,959,318 + +0.2 5,959,318 + -0.1 5,959,319 - +1.0 5,959,319 - +0.4 5,959,319 - +0.4 5,959,319 - -1.4 5,959,319 - +0.5 5,959,319 - -0.3 5,959,319 - +0.2 5,959,320 + -0.2 5,959,321 - +0.0 5,959,379 - -0.4 5,959,379 - -0.5 5,959,387 + +0.0 5,959,387 + -0.9 5,959,388 - -0.4 5,959,388 - -1.0 5,959,523 + +0.8 5,959,523 + -0.7 5,959,523 + -0.0 5,959,524 - -0.8 5,959,532 + -1.2 5,959,533 - +0.7 5,959,556 + -0.6 5,959,556 + +0.5 5,959,557 - +0.3 5,959,557 - +0.1 5,959,718 + PP_5223 0.48 +2.0 5,959,718 + PP_5223 0.48 +1.0 5,959,719 - PP_5223 0.48 -0.1 5,959,851 + PP_5223 0.80 +0.5 5,959,891 + PP_5223 0.90 +0.4 5,959,891 + PP_5223 0.90 -0.5 5,959,891 + PP_5223 0.90 -1.0 5,959,891 + PP_5223 0.90 +0.5 5,959,892 - -0.2 5,959,892 - -1.1 5,959,892 - +2.4 5,960,045 + -0.2 5,960,045 + -0.6 5,960,046 - +0.0 5,960,062 + +0.7 5,960,223 + PP_5224 0.64 -0.1 5,960,224 - PP_5224 0.64 -1.0 5,960,224 - PP_5224 0.64 -0.5 5,960,224 - PP_5224 0.64 -0.3 5,960,266 + PP_5224 0.83 +0.3 5,960,267 - PP_5224 0.84 -0.4 5,960,298 - -0.1 5,960,353 - PP_5225 0.14 +0.6
Or see this region's nucleotide sequence