Strain Fitness in Pseudomonas putida KT2440 around PP_5000

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
5,695,782	-		PP_4999	0.40	+0.5
5,695,782	-		PP_4999	0.40	+1.1
5,696,047	-		PP_4999	0.61	+2.1
5,696,047	-		PP_4999	0.61	+1.1
5,696,055	-		PP_4999	0.61	+2.1
5,696,511	-				+0.7
5,696,511	-				+0.5
5,696,513	-				+0.5
5,696,550	+				+0.3
5,696,550	+				-0.3
5,696,550	+				+0.3
5,696,551	-				-0.5
5,696,559	+				+0.5
5,696,609	+				+1.5
5,696,609	+				-1.1
5,696,649	+				-1.1
5,696,649	+				+0.9
5,696,650	-				+0.1
5,696,650	-				-0.6
5,696,650	-				+0.5
5,696,650	-				-1.3
5,696,711	+				+0.3
5,696,711	+				+0.3
5,696,712	-				-0.9
5,696,712	-				-0.2
5,697,012	-		PP_5000	0.47	-0.4
5,697,145	-		PP_5000	0.73	-0.8
5,697,360	-				+0.6
5,697,360	-				+1.2
5,697,634	+		PP_5001	0.20	+1.7
5,697,634	+				+0.5
5,697,635	-		PP_5001	0.20	+0.0
5,697,843	-		PP_5001	0.35	-2.0
5,697,843	-		PP_5001	0.35	-0.4