Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4906 and PP_4907 are separated by 44 nucleotides PP_4907 and PP_4908 are separated by 9 nucleotides PP_4908 and PP_4909 are separated by 75 nucleotides
PP_4906: PP_4906 - conserved protein of unknown function, at 5,576,279 to 5,577,814
_4906
PP_4907: PP_4907 - Thiosulfate sulfurtransferase, at 5,577,859 to 5,578,668
_4907
PP_4908: PP_4908 - phosphatidylserine decarboxylase, at 5,578,678 to 5,579,541
_4908
PP_4909: PP_4909 - phosphoserine phosphatase, at 5,579,617 to 5,580,864
_4909
Position (kb) 5577
5578
5579 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5576.883 kb on - strand, within PP_4906 at 5576.883 kb on - strand, within PP_4906 at 5577.054 kb on + strand, within PP_4906 at 5577.054 kb on + strand, within PP_4906 at 5577.054 kb on + strand, within PP_4906 at 5577.126 kb on + strand, within PP_4906 at 5577.126 kb on + strand, within PP_4906 at 5577.126 kb on + strand, within PP_4906 at 5577.126 kb on + strand, within PP_4906 at 5577.127 kb on - strand, within PP_4906 at 5577.166 kb on - strand, within PP_4906 at 5577.468 kb on - strand, within PP_4906 at 5577.528 kb on + strand, within PP_4906 at 5577.529 kb on - strand, within PP_4906 at 5577.657 kb on + strand, within PP_4906 at 5577.657 kb on + strand, within PP_4906 at 5577.658 kb on - strand, within PP_4906 at 5577.658 kb on - strand, within PP_4906 at 5577.660 kb on + strand, within PP_4906 at 5577.660 kb on + strand, within PP_4906 at 5577.660 kb on + strand, within PP_4906 at 5577.660 kb on + strand, within PP_4906 at 5577.661 kb on - strand at 5577.661 kb on - strand at 5577.661 kb on - strand at 5577.661 kb on - strand at 5577.796 kb on + strand at 5577.797 kb on - strand at 5577.824 kb on + strand at 5578.108 kb on + strand, within PP_4907 at 5578.108 kb on + strand, within PP_4907 at 5578.108 kb on + strand, within PP_4907 at 5578.108 kb on + strand, within PP_4907 at 5578.117 kb on + strand, within PP_4907 at 5578.186 kb on + strand, within PP_4907 at 5578.186 kb on + strand, within PP_4907 at 5578.186 kb on + strand, within PP_4907 at 5578.192 kb on + strand, within PP_4907 at 5578.192 kb on + strand, within PP_4907 at 5578.280 kb on + strand, within PP_4907 at 5578.570 kb on + strand, within PP_4907 at 5578.570 kb on + strand, within PP_4907 at 5578.612 kb on + strand at 5579.569 kb on - strand at 5579.569 kb on - strand at 5579.569 kb on - strand at 5579.654 kb on + strand at 5579.655 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 5,576,883 - PP_4906 0.39 -0.6 5,576,883 - PP_4906 0.39 -0.7 5,577,054 + PP_4906 0.50 +1.4 5,577,054 + PP_4906 0.50 -0.4 5,577,054 + PP_4906 0.50 -0.5 5,577,126 + PP_4906 0.55 -1.2 5,577,126 + PP_4906 0.55 -0.9 5,577,126 + PP_4906 0.55 +0.7 5,577,126 + PP_4906 0.55 -0.4 5,577,127 - PP_4906 0.55 -1.8 5,577,166 - PP_4906 0.58 +0.4 5,577,468 - PP_4906 0.77 -1.5 5,577,528 + PP_4906 0.81 -0.3 5,577,529 - PP_4906 0.81 -0.9 5,577,657 + PP_4906 0.90 +0.5 5,577,657 + PP_4906 0.90 +1.2 5,577,658 - PP_4906 0.90 -0.8 5,577,658 - PP_4906 0.90 +0.7 5,577,660 + PP_4906 0.90 -0.6 5,577,660 + PP_4906 0.90 +0.6 5,577,660 + PP_4906 0.90 +0.9 5,577,660 + PP_4906 0.90 -0.6 5,577,661 - -1.7 5,577,661 - -0.5 5,577,661 - +0.5 5,577,661 - -0.7 5,577,796 + +0.1 5,577,797 - +0.2 5,577,824 + +0.0 5,578,108 + PP_4907 0.31 -0.6 5,578,108 + PP_4907 0.31 +2.1 5,578,108 + PP_4907 0.31 -0.9 5,578,108 + PP_4907 0.31 -0.5 5,578,117 + PP_4907 0.32 -1.2 5,578,186 + PP_4907 0.40 +0.1 5,578,186 + PP_4907 0.40 -0.1 5,578,186 + PP_4907 0.40 -2.0 5,578,192 + PP_4907 0.41 -1.2 5,578,192 + PP_4907 0.41 +0.2 5,578,280 + PP_4907 0.52 -0.3 5,578,570 + PP_4907 0.88 -1.6 5,578,570 + PP_4907 0.88 -0.1 5,578,612 + -1.4 5,579,569 - +0.3 5,579,569 - +1.6 5,579,569 - +0.5 5,579,654 + +2.1 5,579,655 - -1.5
Or see this region's nucleotide sequence