Strain Fitness in Pseudomonas putida KT2440 around PP_4861

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
5,526,818	+		PP_4860	0.29	+0.6
5,526,819	-		PP_4860	0.29	-0.1
5,526,819	-		PP_4860	0.29	-0.0
5,526,866	+		PP_4860	0.33	-0.0
5,526,866	+		PP_4860	0.33	-1.3
5,526,866	+		PP_4860	0.33	+0.5
5,526,866	+		PP_4860	0.33	-0.1
5,526,866	+		PP_4860	0.33	-0.4
5,526,867	-		PP_4860	0.33	-0.1
5,526,867	-		PP_4860	0.33	+0.2
5,526,869	-		PP_4860	0.34	-0.3
5,526,903	-		PP_4860	0.37	+0.5
5,526,903	-		PP_4860	0.37	+0.0
5,526,995	+		PP_4860	0.45	-1.7
5,526,995	+		PP_4860	0.45	-1.8
5,526,996	-		PP_4860	0.45	-0.5
5,527,086	+		PP_4860	0.53	-0.5
5,527,087	-		PP_4860	0.53	-1.6
5,527,087	-		PP_4860	0.53	+0.5
5,527,210	+		PP_4860	0.64	+0.0
5,527,247	+		PP_4860	0.67	-0.3
5,527,247	+		PP_4860	0.67	-0.8
5,527,248	-		PP_4860	0.67	-0.1
5,527,248	-		PP_4860	0.67	-0.1
5,527,258	+		PP_4860	0.68	-1.8
5,527,258	+		PP_4860	0.68	+0.7
5,527,258	+		PP_4860	0.68	+0.9
5,527,258	+		PP_4860	0.68	-0.3
5,527,258	+		PP_4860	0.68	-0.1
5,527,259	-		PP_4860	0.68	-0.0
5,527,299	+		PP_4860	0.72	-0.0
5,527,299	+		PP_4860	0.72	-0.8
5,527,299	+		PP_4860	0.72	-0.2
5,527,300	-		PP_4860	0.72	-0.4
5,527,300	-		PP_4860	0.72	+0.4
5,527,300	-		PP_4860	0.72	+0.2
5,527,300	-		PP_4860	0.72	-0.2
5,527,300	-		PP_4860	0.72	+1.6
5,527,399	+		PP_4860	0.80	-0.5
5,527,399	+		PP_4860	0.80	+0.1
5,527,400	-		PP_4860	0.80	+0.1
5,527,400	-		PP_4860	0.80	-0.4
5,527,419	-		PP_4860	0.82	+0.4
5,527,432	+		PP_4860	0.83	-0.3
5,527,432	+		PP_4860	0.83	+0.3
5,527,433	-		PP_4860	0.83	-0.6
5,527,433	-		PP_4860	0.83	+0.4
5,527,433	-		PP_4860	0.83	+0.1
5,527,514	+				-0.1
5,527,515	-				-0.3
5,527,515	-				-1.5
5,527,515	-				-1.0
5,527,586	-				+0.3
5,527,619	+				+0.2
5,527,619	+				+2.1
5,527,620	-				-0.7
5,527,620	-				+0.2
5,527,620	-				+0.1
5,527,626	+				-0.2
5,527,627	-				-0.3
5,527,627	-				+1.3
5,527,627	-				+2.1
5,527,683	+				-0.8
5,527,683	+				-0.7
5,527,684	-				-1.3
5,527,716	+				-0.1
5,527,717	-				+0.8
5,527,717	-				-0.4
5,527,785	+		PP_4861	0.24	-1.0
5,527,785	+		PP_4861	0.24	-1.5
5,527,786	-		PP_4861	0.25	-0.4
5,527,786	-		PP_4861	0.25	+0.2
5,527,806	-		PP_4861	0.33	-0.7
5,527,836	-		PP_4861	0.45	-1.1
5,527,842	+		PP_4861	0.47	+2.1
5,527,842	+		PP_4861	0.47	-1.0
5,527,842	+		PP_4861	0.47	-0.4
5,527,843	-		PP_4861	0.48	-2.0
5,527,843	-		PP_4861	0.48	-0.7
5,527,926	+		PP_4861	0.81	-0.7
5,527,957	+				+0.3
5,527,957	+				-0.2
5,527,957	+				-0.3
5,527,957	+				-0.2
5,527,957	+				-0.2
5,527,958	-				-0.1
5,527,958	-				-0.5
5,527,961	+				-0.7
5,527,962	-				+1.3
5,527,962	-				-0.8
5,527,963	+				-1.0
5,527,963	+				-1.9
5,527,963	+				+2.2
5,528,017	+				-1.3
5,528,017	+				+0.6
5,528,018	-				-0.0
5,528,023	+				+0.1
5,528,023	+				-0.7
5,528,023	+				+0.1
5,528,024	-				-0.3
5,528,024	-				-1.9
5,528,091	+				+0.3
5,528,142	-		PP_4862	0.16	-0.2
5,528,177	+		PP_4862	0.20	+0.2
5,528,177	+		PP_4862	0.20	-0.2
5,528,185	+				+3.1
5,528,185	+		PP_4862	0.21	-0.2
5,528,185	+		PP_4862	0.21	-0.5
5,528,185	+		PP_4862	0.21	-0.6
5,528,186	-		PP_4862	0.21	+0.2
5,528,333	-		PP_4862	0.41	+0.1
5,528,333	-		PP_4862	0.41	-0.4
5,528,356	+		PP_4862	0.44	-0.4
5,528,356	+		PP_4862	0.44	+0.1
5,528,356	+		PP_4862	0.44	-0.4
5,528,356	+		PP_4862	0.44	+1.1
5,528,356	+		PP_4862	0.44	-0.4
5,528,357	-		PP_4862	0.44	-0.3
5,528,357	-		PP_4862	0.44	+0.3
5,528,357	-		PP_4862	0.44	+0.0
5,528,357	-		PP_4862	0.44	-0.1
5,528,440	+		PP_4862	0.55	-0.4
5,528,440	+		PP_4862	0.55	+0.5
5,528,440	+		PP_4862	0.55	+0.3
5,528,441	-		PP_4862	0.55	-0.9
5,528,441	-		PP_4862	0.55	+0.3
5,528,441	-		PP_4862	0.55	+0.1
5,528,441	-		PP_4862	0.55	+0.1
5,528,441	-		PP_4862	0.55	-0.7
5,528,441	-		PP_4862	0.55	+1.2
5,528,441	-		PP_4862	0.55	-1.4
5,528,582	+		PP_4862	0.73	+0.1
5,528,582	+		PP_4862	0.73	+2.1
5,528,582	+		PP_4862	0.73	-0.8
5,528,582	+		PP_4862	0.73	-0.7
5,528,583	-		PP_4862	0.73	-1.3
5,528,697	+		PP_4862	0.88	-0.2
5,528,698	-		PP_4862	0.88	-0.3
5,528,698	-		PP_4862	0.88	-1.0
5,528,773	-				+0.4
5,528,803	+				-0.3
5,528,804	-				-0.3
5,528,804	-				+0.5
5,528,804	-				+0.2
5,528,923	+				-1.4
5,528,924	-				-0.5
5,528,924	-				+1.5