Strain Fitness in Pseudomonas putida KT2440 around PP_4220

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,778,620	+				-0.7
4,778,683	+				+4.1
4,778,738	+				+1.2
4,778,738	+				+0.5
4,778,739	-				+1.0
4,778,739	-				-0.7
4,778,739	-				-0.8
4,778,739	-				-0.4
4,779,067	+				+0.9
4,779,068	-				+1.5
4,779,068	-				+0.1
4,779,068	-				-1.1
4,779,068	-				+1.1
4,779,122	-				-0.4
4,779,381	-				+2.1
4,779,381	-				+1.4
4,779,753	-				+1.0
4,779,863	+				-0.2
4,779,864	-				-0.2
4,779,864	-				+0.4
4,779,864	-				+1.1
4,780,048	+				+0.1
4,780,048	+				+0.8
4,780,049	-				+0.6
4,780,094	+				-1.2
4,780,095	-				-0.3
4,780,095	-				+0.2
4,780,237	+		PP_4220	0.10	+0.7
4,780,409	+		PP_4220	0.12	+0.6
4,780,497	-		PP_4220	0.14	+0.7
4,780,751	-		PP_4220	0.17	+0.2
4,780,803	+		PP_4220	0.17	-0.3
4,780,839	-		PP_4220	0.18	+0.5
4,781,064	-		PP_4220	0.21	+0.7
4,781,171	-		PP_4220	0.22	-0.2
4,781,210	+		PP_4220	0.23	+0.4
4,781,288	+		PP_4220	0.24	-0.1
4,781,301	-		PP_4220	0.24	+0.6
4,781,301	-		PP_4220	0.24	+0.8
4,781,462	+		PP_4220	0.26	+0.2
4,781,516	+		PP_4220	0.26	+0.7
4,781,805	+		PP_4220	0.30	-0.7
4,781,880	-		PP_4220	0.31	+0.0
4,781,984	+		PP_4220	0.32	+0.7
4,781,985	-		PP_4220	0.32	+0.6
4,781,985	-		PP_4220	0.32	-0.6
4,782,167	-		PP_4220	0.35	-0.7
4,782,195	-		PP_4220	0.35	+0.2
4,782,575	+		PP_4220	0.40	+0.5
4,782,575	+		PP_4220	0.40	+0.6
4,782,575	+		PP_4220	0.40	+0.6
4,782,575	+		PP_4220	0.40	+0.1
4,782,587	+		PP_4220	0.40	-1.9
4,782,587	+		PP_4220	0.40	-0.0
4,782,587	+		PP_4220	0.40	+1.5
4,782,587	+		PP_4220	0.40	-0.3
4,782,653	+		PP_4220	0.41	+0.1
4,782,653	+		PP_4220	0.41	-0.2
4,782,653	+		PP_4220	0.41	+0.1
4,782,654	-		PP_4220	0.41	+2.1
4,782,738	+		PP_4220	0.42	-1.1
4,782,738	+		PP_4220	0.42	+0.0
4,782,739	-		PP_4220	0.42	-0.5
4,782,797	+		PP_4220	0.43	+0.1
4,782,848	+		PP_4220	0.43	+0.3
4,782,879	-		PP_4220	0.44	+0.3
4,782,879	-		PP_4220	0.44	-1.4
4,782,906	+		PP_4220	0.44	+1.1
4,783,011	-		PP_4220	0.45	+0.3
4,783,011	-		PP_4220	0.45	+0.6
4,783,076	+		PP_4220	0.46	+1.3
4,783,267	-				+1.1
4,783,529	+		PP_4220	0.52	+0.3
4,783,530	-		PP_4220	0.52	+1.2
4,783,613	+		PP_4220	0.53	+0.1
4,783,813	+		PP_4220	0.56	+0.0
4,783,814	-		PP_4220	0.56	-0.3
4,783,842	+		PP_4220	0.56	+0.1
4,784,208	+		PP_4220	0.61	-0.3
4,784,209	-		PP_4220	0.61	+0.3
4,784,244	+		PP_4220	0.61	+0.9
4,784,317	+		PP_4220	0.62	+0.5
4,784,318	-		PP_4220	0.62	+1.1
4,784,321	+		PP_4220	0.62	+2.0
4,784,322	-		PP_4220	0.62	+2.1
4,784,426	+		PP_4220	0.63	+0.2
4,784,426	+		PP_4220	0.63	-0.1
4,784,426	+		PP_4220	0.63	+0.2
4,784,426	+		PP_4220	0.63	+0.4
4,784,427	-		PP_4220	0.63	-0.4
4,784,427	-		PP_4220	0.63	+0.3
4,784,495	+		PP_4220	0.64	+1.1
4,784,765	+		PP_4220	0.68	+0.7
4,784,796	-		PP_4220	0.68	-0.0
4,784,796	-		PP_4220	0.68	+0.6
4,784,849	+		PP_4220	0.69	-0.5
4,784,850	-		PP_4220	0.69	-0.1
4,784,850	-		PP_4220	0.69	+0.1
4,784,850	-		PP_4220	0.69	+0.6
4,785,119	-		PP_4220	0.72	+0.5
4,785,143	+		PP_4220	0.72	-0.6
4,785,143	+		PP_4220	0.72	-1.5
4,785,143	+		PP_4220	0.72	+1.1
4,785,144	-		PP_4220	0.72	-0.1
4,785,317	+		PP_4220	0.75	-1.2
4,785,318	-		PP_4220	0.75	+0.1
4,785,380	+		PP_4220	0.75	-0.0
4,785,381	-		PP_4220	0.75	-1.4
4,785,389	+		PP_4220	0.76	-0.1
4,785,389	+		PP_4220	0.76	+0.9
4,785,390	-		PP_4220	0.76	-0.1
4,785,738	-		PP_4220	0.80	+1.1
4,785,738	-		PP_4220	0.80	+1.4
4,785,738	-		PP_4220	0.80	+0.2
4,785,738	-		PP_4220	0.80	+0.4
4,785,738	-		PP_4220	0.80	-0.6
4,785,980	+		PP_4220	0.83	+0.9
4,785,980	+		PP_4220	0.83	+0.2
4,785,980	+		PP_4220	0.83	+0.1
4,785,980	+		PP_4220	0.83	+0.8
4,785,981	-		PP_4220	0.83	+0.1
4,785,981	-		PP_4220	0.83	+0.3
4,785,981	-		PP_4220	0.83	+1.2
4,786,161	+		PP_4220	0.85	+2.1
4,786,730	+				-0.3
4,786,731	-				+1.7
4,786,970	+				+0.0
4,786,971	-				+0.5
4,786,971	-				+0.2
4,787,092	+				+1.1
4,787,092	+				+0.1
4,787,097	+				-0.8
4,787,098	-				+0.1
4,787,099	+				+0.1
4,787,153	+				-0.3
4,787,153	+				+0.2
4,787,153	+				-1.0
4,787,154	-				-0.3
4,787,154	-				+0.6
4,787,331	+				+0.7
4,787,449	+				+0.1
4,787,450	-				-0.2
4,787,473	+				-0.2
4,787,474	-				+0.9
4,787,594	-				+0.5
4,787,613	+				-0.2
4,787,702	+				-0.3
4,787,703	-				-0.2
4,787,703	-				+0.1
4,787,724	+				+0.7
4,787,724	+				+0.4
4,787,724	+				+0.4
4,787,724	+				+0.1
4,787,725	-				+0.2
4,787,882	+				+0.9
4,787,883	-				+0.4
4,787,974	-		PP_4221	0.10	+1.2
4,788,245	+		PP_4221	0.14	+0.2
4,788,245	+		PP_4221	0.14	+0.2
4,788,246	-		PP_4221	0.14	-0.5
4,788,246	-		PP_4221	0.14	+2.1
4,788,246	-		PP_4221	0.14	+0.5
4,788,246	-		PP_4221	0.14	-0.6
4,788,246	-		PP_4221	0.14	-0.9
4,788,246	-		PP_4221	0.14	-0.3
4,788,249	+		PP_4221	0.14	-0.1
4,788,249	+		PP_4221	0.14	+0.5
4,788,249	+		PP_4221	0.14	-0.0
4,788,250	-		PP_4221	0.14	+0.2
4,788,300	+		PP_4221	0.15	+0.8
4,788,300	+		PP_4221	0.15	+0.6