Strain Fitness in Pseudomonas putida KT2440 around PP_4218

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,766,855	-		PP_4217	0.62	-3.5
4,766,855	-		PP_4217	0.62	-5.1
4,766,890	+		PP_4217	0.63	-1.9
4,766,890	+		PP_4217	0.63	-4.2
4,766,890	+		PP_4217	0.63	-0.9
4,766,890	+		PP_4217	0.63	-0.6
4,766,890	+		PP_4217	0.63	-3.9
4,766,891	-		PP_4217	0.63	-3.4
4,766,995	+		PP_4217	0.68	-4.6
4,766,995	+		PP_4217	0.68	-3.7
4,766,998	+		PP_4217	0.68	-1.8
4,766,999	-		PP_4217	0.68	-0.1
4,766,999	-		PP_4217	0.68	-2.5
4,767,013	+		PP_4217	0.68	-1.8
4,767,013	+		PP_4217	0.68	-2.5
4,767,014	-		PP_4217	0.68	-2.9
4,767,014	-		PP_4217	0.68	-4.0
4,767,055	+		PP_4217	0.70	-3.4
4,767,056	-		PP_4217	0.70	-4.3
4,767,082	+		PP_4217	0.71	-3.2
4,767,091	+		PP_4217	0.71	-3.4
4,767,091	+		PP_4217	0.71	-2.5
4,767,092	-		PP_4217	0.72	-2.1
4,767,099	+		PP_4217	0.72	-2.7
4,767,100	-		PP_4217	0.72	-2.9
4,767,100	-		PP_4217	0.72	-3.0
4,767,100	-		PP_4217	0.72	-3.2
4,767,167	-		PP_4217	0.75	-2.4
4,767,191	-		PP_4217	0.76	-4.0
4,767,265	-		PP_4217	0.79	-3.3
4,767,265	-		PP_4217	0.79	-0.2
4,767,295	+		PP_4217	0.80	-3.2
4,767,295	+		PP_4217	0.80	-3.6
4,767,296	-		PP_4217	0.80	-3.0
4,767,296	-		PP_4217	0.80	-4.3
4,767,314	+		PP_4217	0.81	-3.6
4,767,314	+		PP_4217	0.81	-1.6
4,767,382	+		PP_4217	0.83	-2.4
4,767,388	+		PP_4217	0.84	-3.6
4,767,388	+		PP_4217	0.84	-4.2
4,767,388	+		PP_4217	0.84	-5.2
4,767,388	+		PP_4217	0.84	-3.4
4,767,389	-		PP_4217	0.84	-2.8
4,767,487	+		PP_4217	0.88	-2.7
4,767,488	-		PP_4217	0.88	-5.4
4,767,488	-		PP_4217	0.88	-1.6
4,767,535	+		PP_4217	0.90	-3.1
4,767,536	-		PP_4217	0.90	-1.9
4,767,536	-		PP_4217	0.90	-2.6
4,767,536	-		PP_4217	0.90	-4.3
4,767,536	-		PP_4217	0.90	-2.0
4,767,536	-		PP_4217	0.90	-4.4
4,767,584	-				-3.1
4,767,584	-				+2.1
4,767,584	-				-3.9
4,767,584	-				-1.1
4,767,604	+				-0.7
4,767,604	+				-1.9
4,767,631	+				-4.9
4,767,632	-				-4.3
4,767,632	-				-2.2
4,767,677	+				-3.2
4,767,677	+				-2.2
4,767,678	-				-3.3
4,767,691	+				-1.9
4,767,691	+				-4.4
4,767,694	+				-3.0
4,767,695	-				-2.3
4,767,739	+				-1.7
4,767,739	+				-3.6
4,767,739	+				-4.2
4,767,739	+				-3.4
4,767,739	+				-0.9
4,767,740	-				-2.8
4,767,740	-				-3.8
4,767,740	-				-4.0
4,767,740	-				-2.8
4,767,775	+				-3.3
4,767,775	+				-4.2
4,767,926	+				-0.2
4,767,927	-				-0.9
4,767,927	-				+0.1
4,768,003	+		PP_4218	0.15	+0.2
4,768,132	+		PP_4218	0.29	+1.4
4,768,132	+		PP_4218	0.29	+3.4
4,768,132	+		PP_4218	0.29	+0.9
4,768,232	-		PP_4218	0.39	+0.3
4,768,239	+		PP_4218	0.40	-0.1
4,768,240	-		PP_4218	0.40	+0.5
4,768,240	-		PP_4218	0.40	+0.6
4,768,240	-		PP_4218	0.40	+0.2
4,768,240	-		PP_4218	0.40	-0.2
4,768,308	-		PP_4218	0.47	+1.9
4,768,528	+		PP_4218	0.70	-0.8
4,768,528	+		PP_4218	0.70	+2.1
4,768,547	-		PP_4218	0.72	+1.5
4,768,595	-		PP_4218	0.77	+0.5
4,768,613	+		PP_4218	0.79	+0.8
4,768,613	+		PP_4218	0.79	+0.5
4,768,614	-		PP_4218	0.79	-0.1
4,768,614	-		PP_4218	0.79	+1.5
4,768,783	+				+0.1
4,768,784	-				-0.3
4,768,908	+				+1.5
4,768,909	-				-0.0
4,768,909	-				-0.2
4,769,032	-				+1.4
4,769,251	-				+0.7
4,769,251	-				+2.1
4,769,584	+				+0.8
4,769,585	-				+0.7