Strain Fitness in Pseudomonas putida KT2440 around PP_3701

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,221,678	+		PP_3700	0.25	+1.3
4,221,696	-		PP_3700	0.26	+0.4
4,221,696	-		PP_3700	0.26	+0.1
4,221,732	+		PP_3700	0.30	-0.1
4,221,733	-		PP_3700	0.30	+1.6
4,221,814	-				-0.4
4,221,847	-				+0.1
4,221,867	-		PP_3700	0.42	-0.6
4,221,870	+		PP_3700	0.42	+1.0
4,221,870	+		PP_3700	0.42	+0.2
4,221,870	+		PP_3700	0.42	-0.1
4,221,904	+		PP_3700	0.45	-2.3
4,221,907	+		PP_3700	0.46	-0.3
4,221,908	-		PP_3700	0.46	+0.4
4,221,909	+		PP_3700	0.46	+0.8
4,221,909	+		PP_3700	0.46	-1.1
4,221,909	+		PP_3700	0.46	+0.7
4,221,912	-		PP_3700	0.46	+0.2
4,221,918	-				+0.6
4,222,046	-				+1.6
4,222,053	+		PP_3700	0.59	-0.5
4,222,169	+		PP_3700	0.69	+0.3
4,222,169	+				-0.2
4,222,169	+		PP_3700	0.69	+0.4
4,222,170	-		PP_3700	0.69	-0.2
4,222,200	+		PP_3700	0.72	-0.0
4,222,228	+		PP_3700	0.75	-0.1
4,222,228	+		PP_3700	0.75	-0.0
4,222,228	+		PP_3700	0.75	+0.4
4,222,228	+		PP_3700	0.75	-2.7
4,222,228	+		PP_3700	0.75	-0.1
4,222,229	-		PP_3700	0.75	+0.4
4,222,229	-		PP_3700	0.75	-0.1
4,222,252	-		PP_3700	0.77	+0.3
4,222,282	+		PP_3700	0.79	-0.0
4,222,282	+		PP_3700	0.79	-0.1
4,222,282	+		PP_3700	0.79	+0.0
4,222,283	-		PP_3700	0.79	+0.1
4,222,283	-		PP_3700	0.79	-0.4
4,222,283	-		PP_3700	0.79	-0.0
4,222,283	-		PP_3700	0.79	+0.6
4,222,403	+				-0.6
4,222,449	+				-0.4
4,222,449	+				-0.5
4,222,449	+				+1.0
4,222,449	+				-0.2
4,222,450	-				+0.1
4,222,450	-				-0.2
4,222,450	-				+0.8
4,222,450	-				-0.8
4,222,451	+				-0.6
4,222,451	+				+0.6
4,222,452	-				-0.5
4,222,452	-				-0.3
4,222,510	+				-0.2
4,222,511	-				-0.2
4,222,511	-				+0.0
4,222,530	+				-1.4
4,222,637	+				-0.4
4,222,637	+				+0.0
4,222,637	+				-0.7
4,222,637	+				+0.8
4,222,638	-				-0.6
4,222,696	+		PP_3701	0.22	-1.4
4,222,696	+		PP_3701	0.22	-0.4
4,222,696	+		PP_3701	0.22	-1.4
4,222,697	-		PP_3701	0.23	-0.2
4,222,697	-		PP_3701	0.23	+0.3
4,222,697	-		PP_3701	0.23	-1.9
4,222,697	-		PP_3701	0.23	-0.4
4,222,697	-		PP_3701	0.23	-0.4
4,222,698	+		PP_3701	0.23	-0.3
4,222,698	+		PP_3701	0.23	-0.4
4,222,699	-		PP_3701	0.24	-0.4
4,222,699	-		PP_3701	0.24	-0.2
4,222,720	+				+0.6
4,222,720	+		PP_3701	0.34	-1.6
4,222,720	+		PP_3701	0.34	-1.3
4,222,862	-				+0.1
4,222,870	+				-0.7
4,222,870	+				+1.3
4,222,871	-				+0.3
4,222,969	+		PP_3702	0.60	-0.8
4,222,995	+				+2.1
4,222,995	+		PP_3702	0.75	+0.5
4,222,995	+		PP_3702	0.75	+1.0
4,222,995	+		PP_3702	0.75	+0.2
4,222,995	+		PP_3702	0.75	-0.6
4,222,996	-		PP_3702	0.76	-0.1
4,222,996	-		PP_3702	0.76	+1.6
4,222,996	-		PP_3702	0.76	-0.7
4,222,996	-		PP_3702	0.76	-0.1
4,223,080	-				+0.5
4,223,095	+				+1.1
4,223,108	+				-1.9
4,223,108	+				-1.1
4,223,108	+				-1.9
4,223,108	+				-0.9
4,223,109	-				-1.3
4,223,109	-				-0.2
4,223,109	-				+1.5
4,223,109	-				-0.1
4,223,140	-				+1.0
4,223,140	-				+0.1
4,223,140	-				-0.4
4,223,140	-				+0.6
4,223,140	-				+0.1
4,223,173	+				-0.9
4,223,174	-		PP_3703	0.10	-0.6
4,223,381	+		PP_3703	0.36	-0.5
4,223,382	-		PP_3703	0.36	+0.0
4,223,431	+		PP_3703	0.42	-1.1
4,223,432	-		PP_3703	0.43	-0.9
4,223,432	-		PP_3703	0.43	+0.1
4,223,432	-		PP_3703	0.43	-0.4
4,223,432	-		PP_3703	0.43	-2.2
4,223,441	+		PP_3703	0.44	-0.4
4,223,478	+		PP_3703	0.48	+0.2
4,223,479	-		PP_3703	0.48	+0.2
4,223,479	-		PP_3703	0.48	+0.8
4,223,535	+		PP_3703	0.55	+0.3
4,223,536	-		PP_3703	0.56	+0.7
4,223,536	-		PP_3703	0.56	-0.4
4,223,587	+		PP_3703	0.62	+0.1
4,223,596	+		PP_3703	0.63	+2.1
4,223,639	+		PP_3703	0.69	+0.3
4,223,640	-		PP_3703	0.69	-0.3
4,223,716	+		PP_3703	0.78	-0.6
4,223,717	-		PP_3703	0.78	+0.9
4,223,717	-		PP_3703	0.78	-0.1
4,223,717	-		PP_3703	0.78	-0.5
4,223,727	+		PP_3703	0.80	+0.1
4,223,728	-		PP_3703	0.80	+0.6
4,223,728	-		PP_3703	0.80	+0.4
4,223,728	-		PP_3703	0.80	-1.1
4,223,734	+		PP_3703	0.81	-0.5
4,223,735	-		PP_3703	0.81	+0.2
4,223,807	+		PP_3703	0.90	-0.1
4,223,852	-				+1.1
4,223,852	-				-0.2