Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3677 and PP_5610 overlap by 20 nucleotides PP_5610 and PP_3678 overlap by 8 nucleotides PP_3678 and PP_3679 are separated by 62 nucleotides PP_3679 and PP_3680 are separated by 237 nucleotides
PP_3677: PP_3677 - conserved protein of unknown function, at 4,177,428 to 4,179,614
_3677
PP_5610: PP_5610 - protein of unknown function, at 4,179,595 to 4,180,143
_5610
PP_3678: PP_3678 - conserved protein of unknown function, at 4,180,136 to 4,181,599
_3678
PP_3679: PP_3679 - conserved protein of unknown function, at 4,181,662 to 4,181,985
_3679
PP_3680: PP_3680 - conserved protein of unknown function, at 4,182,223 to 4,184,226
_3680
Position (kb)
4180
4181
4182 Strain fitness (log2 ratio)
-1
0
1
2 at 4179.236 kb on - strand, within PP_3677 at 4179.279 kb on + strand, within PP_3677 at 4179.279 kb on + strand, within PP_3677 at 4179.318 kb on + strand, within PP_3677 at 4179.478 kb on + strand at 4179.745 kb on - strand, within PP_5610 at 4179.810 kb on + strand, within PP_5610 at 4180.109 kb on + strand at 4180.516 kb on - strand, within PP_3678 at 4180.696 kb on - strand, within PP_3678 at 4180.723 kb on + strand, within PP_3678 at 4180.723 kb on + strand, within PP_3678 at 4180.800 kb on - strand, within PP_3678 at 4180.819 kb on - strand, within PP_3678 at 4180.939 kb on - strand, within PP_3678 at 4181.046 kb on + strand, within PP_3678 at 4181.101 kb on - strand, within PP_3678 at 4181.273 kb on - strand, within PP_3678 at 4181.387 kb on + strand, within PP_3678 at 4181.402 kb on + strand, within PP_3678 at 4181.404 kb on + strand, within PP_3678 at 4181.426 kb on - strand, within PP_3678 at 4181.430 kb on - strand, within PP_3678 at 4181.438 kb on - strand, within PP_3678 at 4181.444 kb on - strand, within PP_3678 at 4181.479 kb on + strand at 4181.480 kb on - strand at 4181.491 kb on - strand at 4181.536 kb on + strand at 4181.545 kb on + strand at 4181.545 kb on + strand at 4181.671 kb on + strand at 4181.698 kb on + strand, within PP_3679 at 4181.699 kb on - strand, within PP_3679 at 4181.699 kb on - strand, within PP_3679 at 4181.819 kb on + strand, within PP_3679 at 4181.882 kb on + strand, within PP_3679 at 4181.882 kb on + strand, within PP_3679 at 4181.882 kb on + strand, within PP_3679 at 4181.885 kb on + strand, within PP_3679 at 4181.886 kb on - strand, within PP_3679 at 4181.923 kb on + strand, within PP_3679 at 4181.924 kb on - strand, within PP_3679 at 4182.020 kb on + strand at 4182.020 kb on + strand at 4182.021 kb on - strand at 4182.021 kb on - strand at 4182.024 kb on + strand at 4182.024 kb on + strand at 4182.024 kb on + strand at 4182.025 kb on - strand at 4182.025 kb on - strand at 4182.025 kb on - strand at 4182.026 kb on + strand at 4182.027 kb on - strand at 4182.027 kb on - strand at 4182.041 kb on + strand at 4182.042 kb on - strand at 4182.054 kb on + strand at 4182.055 kb on - strand at 4182.059 kb on - strand at 4182.061 kb on + strand at 4182.061 kb on + strand at 4182.062 kb on - strand at 4182.112 kb on + strand at 4182.136 kb on + strand at 4182.136 kb on + strand at 4182.137 kb on - strand at 4182.183 kb on - strand at 4182.183 kb on - strand at 4182.207 kb on - strand at 4182.207 kb on - strand at 4182.207 kb on - strand at 4182.241 kb on + strand at 4182.242 kb on - strand at 4182.356 kb on + strand at 4182.356 kb on + strand at 4182.357 kb on - strand at 4182.357 kb on - strand at 4182.357 kb on - strand at 4182.372 kb on + strand at 4182.372 kb on + strand at 4182.372 kb on + strand at 4182.372 kb on + strand at 4182.372 kb on + strand at 4182.373 kb on - strand at 4182.373 kb on - strand at 4182.373 kb on - strand at 4182.373 kb on - strand at 4182.373 kb on - strand at 4182.373 kb on - strand at 4182.376 kb on + strand at 4182.376 kb on + strand at 4182.376 kb on + strand at 4182.377 kb on - strand at 4182.377 kb on - strand at 4182.377 kb on - strand at 4182.383 kb on + strand at 4182.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 4,179,236 - PP_3677 0.83 -1.0 4,179,279 + PP_3677 0.85 +0.0 4,179,279 + PP_3677 0.85 +0.9 4,179,318 + PP_3677 0.86 -1.0 4,179,478 + -1.0 4,179,745 - PP_5610 0.27 +0.3 4,179,810 + PP_5610 0.39 +0.6 4,180,109 + -0.2 4,180,516 - PP_3678 0.26 -0.5 4,180,696 - PP_3678 0.38 -1.5 4,180,723 + PP_3678 0.40 -1.3 4,180,723 + PP_3678 0.40 -1.4 4,180,800 - PP_3678 0.45 -0.3 4,180,819 - PP_3678 0.47 -0.4 4,180,939 - PP_3678 0.55 +0.0 4,181,046 + PP_3678 0.62 -1.7 4,181,101 - PP_3678 0.66 -0.4 4,181,273 - PP_3678 0.78 -1.3 4,181,387 + PP_3678 0.85 +0.1 4,181,402 + PP_3678 0.86 -0.7 4,181,404 + PP_3678 0.87 -0.5 4,181,426 - PP_3678 0.88 -0.0 4,181,430 - PP_3678 0.88 -1.1 4,181,438 - PP_3678 0.89 -0.6 4,181,444 - PP_3678 0.89 -0.4 4,181,479 + -0.3 4,181,480 - -0.7 4,181,491 - +0.2 4,181,536 + -0.2 4,181,545 + +0.3 4,181,545 + -0.3 4,181,671 + +1.8 4,181,698 + PP_3679 0.11 -0.7 4,181,699 - PP_3679 0.11 +0.7 4,181,699 - PP_3679 0.11 -0.2 4,181,819 + PP_3679 0.48 -0.0 4,181,882 + PP_3679 0.68 +1.7 4,181,882 + PP_3679 0.68 -0.7 4,181,882 + PP_3679 0.68 -1.5 4,181,885 + PP_3679 0.69 +1.1 4,181,886 - PP_3679 0.69 +1.2 4,181,923 + PP_3679 0.81 -1.0 4,181,924 - PP_3679 0.81 -1.2 4,182,020 + -0.6 4,182,020 + -0.0 4,182,021 - -0.3 4,182,021 - +1.2 4,182,024 + +0.5 4,182,024 + +0.3 4,182,024 + +0.1 4,182,025 - -0.1 4,182,025 - -0.1 4,182,025 - +0.0 4,182,026 + +0.3 4,182,027 - +0.4 4,182,027 - -0.0 4,182,041 + +0.4 4,182,042 - -0.9 4,182,054 + -0.5 4,182,055 - +0.9 4,182,059 - -0.7 4,182,061 + -0.8 4,182,061 + -0.9 4,182,062 - +0.6 4,182,112 + +0.8 4,182,136 + -0.1 4,182,136 + +0.4 4,182,137 - +0.0 4,182,183 - +1.3 4,182,183 - +0.8 4,182,207 - -1.0 4,182,207 - +1.3 4,182,207 - +0.3 4,182,241 + +0.1 4,182,242 - -0.8 4,182,356 + -0.5 4,182,356 + -0.1 4,182,357 - +0.3 4,182,357 - +0.4 4,182,357 - +0.3 4,182,372 + -0.5 4,182,372 + +0.3 4,182,372 + -0.5 4,182,372 + -1.1 4,182,372 + +0.1 4,182,373 - +0.5 4,182,373 - +0.2 4,182,373 - -1.1 4,182,373 - -1.1 4,182,373 - -1.1 4,182,373 - -1.3 4,182,376 + +0.6 4,182,376 + -0.0 4,182,376 + -1.0 4,182,377 - +0.8 4,182,377 - +0.1 4,182,377 - +1.0 4,182,383 + -0.4 4,182,384 - -0.5
Or see this region's nucleotide sequence