Strain Fitness in Pseudomonas putida KT2440 around PP_3514

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,985,024	-		PP_3513	0.36	+0.2
3,985,024	-		PP_3513	0.36	-1.8
3,985,024	-		PP_3513	0.36	+0.1
3,985,024	-		PP_3513	0.36	-1.2
3,985,031	-		PP_3513	0.37	-0.3
3,985,117	+		PP_3513	0.47	-0.5
3,985,117	+		PP_3513	0.47	+0.2
3,985,118	-		PP_3513	0.47	+0.2
3,985,118	-		PP_3513	0.47	-0.4
3,985,118	-		PP_3513	0.47	+0.1
3,985,230	+		PP_3513	0.60	+0.0
3,985,231	-		PP_3513	0.60	-0.4
3,985,231	-		PP_3513	0.60	-0.0
3,985,305	+		PP_3513	0.69	-0.4
3,985,305	+		PP_3513	0.69	-0.5
3,985,305	+		PP_3513	0.69	-0.2
3,985,305	+		PP_3513	0.69	-0.3
3,985,305	+		PP_3513	0.69	-0.1
3,985,305	+		PP_3513	0.69	-0.5
3,985,306	-		PP_3513	0.69	-0.0
3,985,306	-		PP_3513	0.69	+0.6
3,985,306	-		PP_3513	0.69	+0.3
3,985,306	-		PP_3513	0.69	-0.6
3,985,306	-		PP_3513	0.69	-0.1
3,985,306	-		PP_3513	0.69	-0.2
3,985,306	-		PP_3513	0.69	+0.2
3,985,306	-		PP_3513	0.69	-0.8
3,985,307	+		PP_3513	0.69	+1.2
3,985,317	+		PP_3513	0.70	-0.8
3,985,317	+		PP_3513	0.70	-0.0
3,985,317	+		PP_3513	0.70	-0.3
3,985,318	-				+1.1
3,985,318	-		PP_3513	0.70	-1.1
3,985,318	-		PP_3513	0.70	+0.2
3,985,318	-		PP_3513	0.70	-1.5
3,985,369	-		PP_3513	0.76	-1.3
3,985,433	-		PP_3513	0.84	-0.8
3,985,493	+				-1.0
3,985,569	+				+0.6
3,985,569	+				-0.7
3,985,569	+				-0.3
3,985,570	-				-0.8
3,985,696	-				+0.7
3,985,723	+				-1.1
3,985,723	+				-1.0
3,985,724	-				-0.3
3,985,724	-				-0.7
3,985,724	-				+0.8
3,985,724	-				-0.7
3,985,724	-				-0.2
3,985,735	-				-0.5
3,985,988	+		PP_3514	0.15	-0.4
3,986,007	-		PP_3514	0.16	+0.3
3,986,007	-		PP_3514	0.16	-0.3
3,986,020	+		PP_3514	0.17	-0.7
3,986,039	+		PP_3514	0.18	-0.5
3,986,039	+		PP_3514	0.18	-0.8
3,986,040	-		PP_3514	0.18	+0.9
3,986,051	+		PP_3514	0.19	+0.9
3,986,052	-		PP_3514	0.19	-0.6
3,986,055	-		PP_3514	0.19	-0.2
3,986,055	-		PP_3514	0.19	+0.2
3,986,129	+		PP_3514	0.23	-0.1
3,986,130	-		PP_3514	0.23	-0.6
3,986,333	+		PP_3514	0.35	+0.3
3,986,333	+		PP_3514	0.35	-1.0
3,986,334	-		PP_3514	0.35	-0.7
3,986,429	+		PP_3514	0.40	+0.4
3,986,429	+		PP_3514	0.40	-0.8
3,986,429	+		PP_3514	0.40	+0.7
3,986,430	-		PP_3514	0.40	-0.8
3,986,430	-		PP_3514	0.40	-0.4
3,986,552	+		PP_3514	0.47	-1.1
3,986,552	+		PP_3514	0.47	+0.1
3,986,552	+		PP_3514	0.47	-0.4
3,986,553	-		PP_3514	0.47	-1.5
3,986,568	-		PP_3514	0.48	-0.6
3,986,594	+		PP_3514	0.50	+0.3
3,986,594	+		PP_3514	0.50	-0.2
3,986,595	-		PP_3514	0.50	-0.2
3,986,595	-		PP_3514	0.50	-0.3
3,986,648	+		PP_3514	0.53	-0.6
3,986,649	-		PP_3514	0.53	-0.0
3,986,659	-		PP_3514	0.54	-0.2
3,986,683	+				-0.3
3,986,867	+		PP_3514	0.65	-0.9
3,986,867	+		PP_3514	0.65	-0.6
3,986,868	-		PP_3514	0.66	-0.8
3,986,980	+		PP_3514	0.72	-0.8
3,987,023	+		PP_3514	0.74	-0.9
3,987,240	-		PP_3514	0.87	+0.4
3,987,345	+				-0.8
3,987,496	-				-0.4
3,987,496	-				-0.8
3,987,816	+		PP_3515	0.16	+0.2
3,988,105	-		PP_3515	0.30	+0.5
3,988,161	+		PP_3515	0.33	+0.2
3,988,161	+		PP_3515	0.33	-1.1
3,988,161	+		PP_3515	0.33	+0.1
3,988,162	-		PP_3515	0.33	+0.3
3,988,162	-		PP_3515	0.33	-0.3
3,988,214	-		PP_3515	0.35	+0.6
3,988,218	+		PP_3515	0.35	+1.5
3,988,219	-		PP_3515	0.35	+0.0
3,988,219	-		PP_3515	0.35	+0.3
3,988,356	-		PP_3515	0.42	+0.5