Strain Fitness in Pseudomonas putida KT2440 around PP_3190

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,618,622	-		PP_3189	0.38	-1.4
3,618,894	+		PP_3189	0.60	-0.7
3,618,894	+		PP_3189	0.60	-1.2
3,618,912	+		PP_3189	0.61	+0.2
3,618,913	-		PP_3189	0.61	-1.1
3,619,002	+		PP_3189	0.69	+0.0
3,619,095	+		PP_3189	0.76	+0.1
3,619,116	-		PP_3189	0.78	+0.3
3,619,116	-		PP_3189	0.78	-1.5
3,619,164	+		PP_3189	0.82	+0.1
3,619,243	+		PP_3189	0.88	+1.0
3,619,243	+		PP_3189	0.88	-0.2
3,619,244	-		PP_3189	0.88	-0.1
3,619,294	-				+0.3
3,619,482	+				-0.1
3,619,482	+				+0.4
3,619,702	-		PP_3190	0.31	-1.3
3,619,847	+		PP_3190	0.47	-1.1
3,620,135	+		PP_3190	0.77	-0.4
3,620,549	+		PP_3191	0.19	+0.1
3,620,829	+		PP_3191	0.46	+0.3
3,620,866	+		PP_3191	0.49	-1.2
3,620,866	+		PP_3191	0.49	+0.7
3,620,866	+		PP_3191	0.49	-0.0
3,620,883	+		PP_3191	0.51	+1.3
3,621,013	+		PP_3191	0.63	-0.2
3,621,014	-		PP_3191	0.63	+0.8
3,621,014	-		PP_3191	0.63	-0.2
3,621,299	+				-0.5
3,621,300	-				-0.4
3,621,300	-				+0.2
3,621,300	-				+0.4