Strain Fitness in Pseudomonas putida KT2440 around PP_3019

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,405,389	-		PP_3016	0.89	-0.4
3,405,519	+				-0.8
3,405,519	+				+0.6
3,405,520	-				+0.6
3,405,633	+		PP_3017	0.16	+0.5
3,405,633	+		PP_3017	0.16	-0.9
3,405,633	+		PP_3017	0.16	+2.1
3,405,664	-		PP_3017	0.22	+0.0
3,405,703	-		PP_3017	0.30	+1.3
3,405,734	+		PP_3017	0.36	-0.3
3,405,848	+		PP_3017	0.59	-0.2
3,405,848	+				+0.5
3,405,849	-		PP_3017	0.59	-1.4
3,405,849	-		PP_3017	0.59	-0.6
3,406,007	+				-0.6
3,406,008	-				+0.6
3,406,053	-				+0.1
3,406,053	-				+0.3
3,406,124	-		PP_3018	0.27	+0.0
3,406,258	+		PP_3018	0.82	+0.5
3,406,258	+		PP_3018	0.82	+1.2
3,406,258	+		PP_3018	0.82	+1.5
3,406,259	-		PP_3018	0.82	-0.7
3,406,259	-		PP_3018	0.82	-0.5
3,406,271	-		PP_3018	0.87	-0.5
3,406,271	-		PP_3018	0.87	-0.4
3,406,285	-				+0.1
3,406,294	+				+0.0
3,406,295	-				-0.1
3,406,295	-				+0.4
3,406,355	+				-0.7
3,406,476	-		PP_3019	0.24	-0.9
3,406,621	-		PP_3019	0.43	-1.3
3,406,689	+		PP_3019	0.53	-1.1
3,406,689	+		PP_3019	0.53	-1.6
3,406,689	+				+2.1
3,406,690	-		PP_3019	0.53	+1.0
3,406,690	-		PP_3019	0.53	-1.8
3,406,690	-		PP_3019	0.53	-0.0
3,406,725	+		PP_3019	0.57	-0.4
3,406,725	+		PP_3019	0.57	-2.0
3,406,772	+		PP_3019	0.64	-0.2
3,406,773	-		PP_3019	0.64	-0.6
3,407,024	+				+0.2
3,407,079	+				+0.8
3,407,079	+				+0.2
3,407,080	-				-1.1
3,407,080	-				+0.1
3,407,080	-				+0.2
3,407,080	-				-0.0
3,407,080	-				-0.0
3,407,080	-				+0.6
3,407,082	+				-0.7
3,407,083	-				-0.0
3,407,169	+				-0.4
3,407,169	+				-0.3
3,407,169	+				-0.1
3,407,235	+				+0.6
3,407,254	+				-0.1
3,407,255	-				+0.3
3,407,255	-				+0.2
3,407,255	-				-1.1
3,407,654	-		PP_3020	0.55	+0.2
3,407,698	+		PP_3020	0.61	-0.7
3,407,698	+		PP_3020	0.61	-0.7
3,407,699	-		PP_3020	0.61	+0.7
3,407,699	-		PP_3020	0.61	-0.1