Strain Fitness in Pseudomonas putida KT2440 around PP_2667

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
3,053,906	+				+0.5
3,053,953	+				+0.8
3,053,953	+				+0.3
3,053,954	-				-0.2
3,054,039	-		PP_2666	0.13	-0.4
3,054,054	+		PP_2666	0.16	-0.1
3,054,055	-		PP_2666	0.16	+0.2
3,054,055	-		PP_2666	0.16	+0.2
3,054,095	-		PP_2666	0.23	+0.6
3,054,165	+		PP_2666	0.36	-0.4
3,054,166	-		PP_2666	0.36	-0.1
3,054,176	+				+0.8
3,054,176	+		PP_2666	0.38	+0.5
3,054,177	-		PP_2666	0.38	+1.0
3,054,177	-		PP_2666	0.38	-2.2
3,054,262	+		PP_2666	0.53	-0.3
3,054,263	-		PP_2666	0.53	-0.5
3,054,457	-		PP_2666	0.88	-0.6
3,054,457	-		PP_2666	0.88	+0.3
3,054,460	+		PP_2666	0.88	+0.1
3,054,460	+		PP_2666	0.88	+0.5
3,054,460	+		PP_2666	0.88	+0.4
3,054,461	-		PP_2666	0.88	-1.4
3,054,545	-				+0.4
3,054,555	+				+0.2
3,054,555	+				-0.0
3,054,556	-				+0.4
3,054,556	-				-0.3
3,054,556	-				-0.1
3,054,597	+				-0.0
3,054,597	+				-0.2
3,054,598	-				+0.2
3,054,615	-				+0.5
3,054,618	-				+0.7
3,054,618	-				+0.4
3,054,761	+				-0.2
3,054,762	-				+0.4
3,054,762	-				-0.3
3,054,762	-				-0.0
3,055,048	-		PP_2667	0.27	-0.0
3,055,172	+		PP_2667	0.43	+0.6
3,055,173	-		PP_2667	0.43	-0.6
3,055,224	+		PP_2667	0.49	+0.8
3,055,227	-		PP_2667	0.50	+0.0
3,055,251	+		PP_2667	0.53	-0.1
3,055,251	+		PP_2667	0.53	-0.5
3,055,252	-		PP_2667	0.53	+0.1
3,055,270	+		PP_2667	0.55	-0.1
3,055,431	+		PP_2667	0.76	-0.2
3,055,431	+		PP_2667	0.76	+0.3
3,055,432	-		PP_2667	0.76	+0.4
3,055,432	-		PP_2667	0.76	-1.3
3,055,432	-		PP_2667	0.76	+0.9
3,055,449	+		PP_2667	0.78	-0.2
3,055,449	+		PP_2667	0.78	-0.6
3,055,449	+		PP_2667	0.78	-0.4
3,055,449	+		PP_2667	0.78	-0.4
3,055,450	-		PP_2667	0.78	+0.2
3,055,450	-		PP_2667	0.78	+1.2
3,055,450	-		PP_2667	0.78	+0.1
3,055,450	-		PP_2667	0.78	-0.7
3,055,450	-		PP_2667	0.78	-2.1
3,055,450	-		PP_2667	0.78	+0.6
3,055,450	-		PP_2667	0.78	-0.2
3,055,450	-		PP_2667	0.78	-1.6
3,055,556	-				-0.7
3,055,556	-				+0.1
3,055,615	-				-1.5
3,055,892	-		PP_2668	0.37	+0.8
3,056,204	+		PP_2668	0.79	+0.8
3,056,204	+		PP_2668	0.79	-1.5
3,056,205	-		PP_2668	0.79	-0.4
3,056,603	-		PP_2669	0.25	-0.3
3,056,603	-		PP_2669	0.25	-0.5
3,056,603	-		PP_2669	0.25	-0.6