Strain Fitness in Pseudomonas putida KT2440 around PP_2615

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,990,844	+		PP_2614	0.71	-0.9
2,990,845	-		PP_2614	0.71	+0.6
2,991,209	-		PP_2614	0.88	-0.2
2,991,212	-		PP_2614	0.88	-0.1
2,991,212	-				+1.2
2,991,212	-		PP_2614	0.88	+2.2
2,991,212	-		PP_2614	0.88	+0.6
2,991,600	-				+0.3
2,991,600	-				-1.1
2,991,826	-		PP_2615	0.53	-1.0
2,991,826	-		PP_2615	0.53	+0.7
2,991,839	-		PP_2615	0.55	-2.4
2,992,270	+				+1.5
2,992,270	+				+0.2
2,992,270	+				-0.3
2,992,270	+				+0.3
2,992,373	-		PP_2616	0.20	+0.5
2,992,681	-		PP_2616	0.55	-0.4
2,992,700	-		PP_2616	0.57	-0.7
2,992,706	-		PP_2616	0.58	-1.0
2,992,776	+		PP_2616	0.66	-0.4
2,992,776	+		PP_2616	0.66	+1.0
2,992,820	+		PP_2616	0.71	-1.5
2,992,820	+		PP_2616	0.71	+0.5
2,993,054	+				+0.2
2,993,055	-				+0.2
2,993,055	-				-0.0
2,993,055	-				-0.1