Strain Fitness in Pseudomonas putida KT2440 around PP_1748

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,945,568	-		PP_1745	0.55	-0.6
1,945,568	-		PP_1745	0.55	+0.7
1,945,774	+		PP_1745	0.81	-0.5
1,945,774	+		PP_1745	0.81	-1.9
1,945,774	+		PP_1745	0.81	-0.2
1,945,774	+		PP_1745	0.81	-0.5
1,945,774	+		PP_1745	0.81	-0.3
1,945,774	+		PP_1745	0.81	-0.5
1,945,776	+		PP_1745	0.81	+1.9
1,945,777	-		PP_1745	0.81	+0.2
1,945,873	+				+1.7
1,945,874	-				-0.2
1,945,939	-				-1.2
1,945,939	-				-0.7
1,945,944	+				+0.4
1,945,944	+				+0.1
1,945,944	+				+0.3
1,945,944	+				+0.1
1,945,945	-				+0.5
1,945,945	-				-0.7
1,945,945	-				+0.7
1,945,945	-				-0.5
1,945,945	-				-0.6
1,945,945	-				-0.3
1,945,945	-				-1.4
1,945,948	+				+0.8
1,945,948	+				-0.4
1,945,949	-				+0.3
1,945,949	-				+0.2
1,945,949	-				-1.1
1,945,960	+				+0.1
1,945,961	-				-1.2
1,945,961	-				-2.0
1,945,961	-				-0.7
1,945,987	+				+2.1
1,945,988	-				-0.7
1,945,994	+				-0.8
1,945,995	-				-0.2
1,946,191	+		PP_1746	0.78	+0.1
1,946,356	-		PP_1747	0.49	-0.5
1,946,459	-				+0.5
1,946,492	-				+0.1
1,946,496	-				+1.1
1,946,506	+				-0.3
1,946,506	+				-0.7
1,946,656	+				-0.3
1,946,657	-				-0.0
1,946,657	-				-0.1
1,946,704	-		PP_1748	0.13	-0.1
1,946,704	-		PP_1748	0.13	-1.1
1,946,804	-		PP_1748	0.21	-2.6
1,946,919	+		PP_1748	0.31	-1.8
1,946,919	+		PP_1748	0.31	+0.3
1,946,926	-		PP_1748	0.31	-0.8
1,947,153	-		PP_1748	0.50	-0.1
1,947,283	-		PP_1748	0.61	-0.8
1,947,633	+				-0.0
1,947,634	-				+1.5
1,947,825	+				-1.1
1,947,848	+				+0.2
1,948,019	-				+0.5
1,948,214	-		PP_1749	0.17	-1.1
1,948,337	+		PP_1749	0.25	-0.3