Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1326 and PP_1328 are separated by 733 nucleotides PP_1328 and PP_1329 overlap by 4 nucleotides PP_1329 and PP_1330 overlap by 4 nucleotides
PP_1326: PP_1326 - 16S rRNA 2'-O-ribose C1402 methyltransferase, at 1,511,744 to 1,512,628
_1326
PP_1328: PP_1328 - Protein MraZ, at 1,513,362 to 1,513,835
_1328
PP_1329: PP_1329 - Ribosomal RNA small subunit methyltransferase H, at 1,513,832 to 1,514,779
_1329
PP_1330: PP_1330 - Cell division protein FtsL, at 1,514,776 to 1,515,069
_1330
Position (kb)
1513
1514 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1512.519 kb on + strand, within PP_1326 at 1512.519 kb on + strand, within PP_1326 at 1512.593 kb on + strand at 1512.593 kb on + strand at 1512.593 kb on + strand at 1512.594 kb on - strand at 1512.594 kb on - strand at 1512.594 kb on - strand at 1512.626 kb on + strand at 1512.642 kb on + strand at 1512.642 kb on + strand at 1512.643 kb on - strand at 1513.095 kb on + strand at 1513.138 kb on + strand at 1513.235 kb on + strand at 1513.250 kb on + strand at 1513.299 kb on + strand at 1513.299 kb on + strand at 1513.299 kb on + strand at 1513.301 kb on + strand at 1513.301 kb on + strand at 1513.357 kb on + strand at 1513.546 kb on + strand, within PP_1328 at 1513.546 kb on + strand, within PP_1328 at 1513.546 kb on + strand, within PP_1328 at 1513.546 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 1,512,519 + PP_1326 0.88 -1.7 1,512,519 + PP_1326 0.88 -2.0 1,512,593 + +2.1 1,512,593 + -1.4 1,512,593 + -0.7 1,512,594 - +0.5 1,512,594 - -0.6 1,512,594 - -0.7 1,512,626 + +0.4 1,512,642 + -1.1 1,512,642 + +0.2 1,512,643 - -0.2 1,513,095 + -0.1 1,513,138 + +1.1 1,513,235 + +0.1 1,513,250 + -3.2 1,513,299 + -2.0 1,513,299 + -3.2 1,513,299 + -0.9 1,513,301 + -0.7 1,513,301 + -2.1 1,513,357 + -1.2 1,513,546 + PP_1328 0.39 -1.8 1,513,546 + PP_1328 0.39 -2.4 1,513,546 + PP_1328 0.39 -2.8 1,513,546 + +0.1
Or see this region's nucleotide sequence