Strain Fitness in Pseudomonas putida KT2440 around PP_1111

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
1,269,759	-		PP_1110	0.21	-4.4
1,269,862	+				+0.1
1,269,902	-		PP_1110	0.41	-1.8
1,269,910	-		PP_1110	0.42	-4.2
1,270,084	+		PP_1110	0.67	-1.9
1,270,085	-		PP_1110	0.67	-3.5
1,270,249	-				-3.6
1,270,329	+				+0.1
1,270,363	-				-0.5
1,270,437	-		PP_1111	0.16	-4.7
1,270,544	+		PP_1111	0.29	-2.9
1,270,590	-		PP_1111	0.35	-3.7
1,270,590	-		PP_1111	0.35	+1.1
1,270,661	+		PP_1111	0.44	+0.1
1,270,719	+		PP_1111	0.51	-1.1
1,270,719	+		PP_1111	0.51	+1.1
1,270,775	+		PP_1111	0.58	+2.1
1,270,897	-		PP_1111	0.74	-3.6
1,270,897	-		PP_1111	0.74	-2.8
1,270,935	+		PP_1111	0.79	+1.1
1,270,935	+		PP_1111	0.79	-0.3
1,270,936	-		PP_1111	0.79	-4.0
1,270,936	-		PP_1111	0.79	-3.0
1,270,936	-		PP_1111	0.79	-4.9
1,270,936	-		PP_1111	0.79	-4.0
1,270,983	-		PP_1111	0.85	-2.0
1,270,983	-		PP_1111	0.85	-2.6
1,271,024	+				+2.1
1,271,083	-				-4.6
1,271,083	-				-4.7
1,271,099	-				-0.2
1,271,099	-				+0.7
1,271,099	-				+0.5
1,271,099	-				-0.2
1,271,126	+				-0.2
1,271,127	-				+0.2
1,271,132	+				+0.3
1,271,132	+				-0.0
1,271,133	-				+0.5
1,271,429	+		PP_1112	0.27	-0.2
1,271,429	+		PP_1112	0.27	-1.0
1,271,430	-		PP_1112	0.27	+1.2
1,271,430	-		PP_1112	0.27	-0.3
1,271,430	-		PP_1112	0.27	+0.5
1,271,430	-		PP_1112	0.27	+0.4
1,271,430	-		PP_1112	0.27	+1.2
1,271,554	+		PP_1112	0.38	+3.6
1,271,603	+		PP_1112	0.42	-0.6
1,271,604	-		PP_1112	0.42	+1.1
1,271,924	-		PP_1112	0.68	-1.3
1,271,980	+		PP_1112	0.73	-0.5
1,271,980	+		PP_1112	0.73	+0.2
1,271,980	+		PP_1112	0.73	-0.3
1,271,981	-		PP_1112	0.73	+0.0
1,271,997	-		PP_1112	0.74	+0.9
1,272,050	+		PP_1112	0.78	-0.9
1,272,050	+		PP_1112	0.78	-0.6
1,272,050	+		PP_1112	0.78	-0.1
1,272,050	+		PP_1112	0.78	+0.4
1,272,050	+		PP_1112	0.78	+2.1
1,272,051	-		PP_1112	0.79	+0.2
1,272,051	-		PP_1112	0.79	+0.2
1,272,051	-		PP_1112	0.79	+0.4
1,272,051	-		PP_1112	0.79	+0.9
1,272,051	-		PP_1112	0.79	+0.6
1,272,051	-		PP_1112	0.79	+0.2
1,272,051	-		PP_1112	0.79	+0.9
1,272,055	-		PP_1112	0.79	+0.7