Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0709 and PP_0710 are separated by 92 nucleotides PP_0710 and PP_0711 are separated by 211 nucleotides PP_0711 and PP_0712 are separated by 106 nucleotides
PP_0709: PP_0709 - Transporter, NCS1 nucleoside transporter family, at 824,617 to 826,116
_0709
PP_0710: PP_0710 - conserved protein of unknown function, at 826,209 to 826,598
_0710
PP_0711: PP_0711 - putative hydrolase, at 826,810 to 827,430
_0711
PP_0712: PP_0712 - polyphosphate kinase 2, at 827,537 to 828,352
_0712
Position (kb)
826
827 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 825.246 kb on + strand, within PP_0709 at 825.247 kb on - strand, within PP_0709 at 825.275 kb on + strand, within PP_0709 at 825.275 kb on + strand, within PP_0709 at 825.275 kb on + strand, within PP_0709 at 825.275 kb on + strand, within PP_0709 at 825.275 kb on + strand, within PP_0709 at 825.275 kb on + strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.276 kb on - strand, within PP_0709 at 825.293 kb on + strand, within PP_0709 at 825.294 kb on - strand, within PP_0709 at 825.294 kb on - strand, within PP_0709 at 825.327 kb on + strand, within PP_0709 at 825.327 kb on + strand, within PP_0709 at 825.328 kb on - strand, within PP_0709 at 825.419 kb on + strand, within PP_0709 at 825.420 kb on - strand, within PP_0709 at 825.649 kb on + strand, within PP_0709 at 825.649 kb on + strand, within PP_0709 at 825.650 kb on - strand, within PP_0709 at 825.650 kb on - strand, within PP_0709 at 825.681 kb on - strand, within PP_0709 at 825.830 kb on - strand, within PP_0709 at 825.830 kb on - strand, within PP_0709 at 826.141 kb on + strand at 826.142 kb on - strand at 826.199 kb on + strand at 826.374 kb on + strand, within PP_0710 at 826.374 kb on + strand, within PP_0710 at 826.374 kb on + strand, within PP_0710 at 826.374 kb on + strand, within PP_0710 at 826.374 kb on + strand, within PP_0710 at 826.374 kb on + strand, within PP_0710 at 826.374 kb on + strand, within PP_0710 at 826.375 kb on - strand, within PP_0710 at 826.375 kb on - strand, within PP_0710 at 826.375 kb on - strand, within PP_0710 at 826.375 kb on - strand, within PP_0710 at 826.375 kb on - strand, within PP_0710 at 826.410 kb on - strand, within PP_0710 at 826.447 kb on + strand, within PP_0710 at 826.777 kb on + strand at 826.778 kb on - strand at 826.853 kb on + strand at 826.955 kb on - strand, within PP_0711 at 827.160 kb on - strand, within PP_0711 at 827.160 kb on - strand, within PP_0711 at 827.160 kb on - strand, within PP_0711 at 827.160 kb on - strand, within PP_0711 at 827.380 kb on - strand at 827.410 kb on + strand at 827.410 kb on + strand at 827.428 kb on + strand at 827.429 kb on - strand at 827.495 kb on - strand at 827.495 kb on - strand at 827.504 kb on + strand at 827.504 kb on + strand at 827.504 kb on + strand at 827.504 kb on + strand at 827.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 825,246 + PP_0709 0.42 -0.4 825,247 - PP_0709 0.42 +0.5 825,275 + PP_0709 0.44 +0.2 825,275 + PP_0709 0.44 +0.9 825,275 + PP_0709 0.44 +2.1 825,275 + PP_0709 0.44 -0.5 825,275 + PP_0709 0.44 -0.8 825,275 + PP_0709 0.44 +1.1 825,276 - PP_0709 0.44 +0.6 825,276 - PP_0709 0.44 +0.2 825,276 - PP_0709 0.44 -0.0 825,276 - PP_0709 0.44 -1.5 825,276 - PP_0709 0.44 -0.0 825,276 - PP_0709 0.44 -0.3 825,276 - PP_0709 0.44 +1.1 825,276 - PP_0709 0.44 -0.7 825,293 + PP_0709 0.45 -0.7 825,294 - PP_0709 0.45 -0.6 825,294 - PP_0709 0.45 -0.3 825,327 + PP_0709 0.47 -0.6 825,327 + PP_0709 0.47 -0.8 825,328 - PP_0709 0.47 +0.8 825,419 + PP_0709 0.53 +0.1 825,420 - PP_0709 0.54 -1.0 825,649 + PP_0709 0.69 +0.8 825,649 + PP_0709 0.69 +1.1 825,650 - PP_0709 0.69 -0.5 825,650 - PP_0709 0.69 -1.4 825,681 - PP_0709 0.71 -0.7 825,830 - PP_0709 0.81 +1.4 825,830 - PP_0709 0.81 +0.7 826,141 + -0.5 826,142 - -0.8 826,199 + +0.0 826,374 + PP_0710 0.42 -0.6 826,374 + PP_0710 0.42 -0.9 826,374 + PP_0710 0.42 +0.1 826,374 + PP_0710 0.42 -1.3 826,374 + PP_0710 0.42 -1.9 826,374 + PP_0710 0.42 -1.0 826,374 + PP_0710 0.42 +1.3 826,375 - PP_0710 0.43 -1.6 826,375 - PP_0710 0.43 -1.6 826,375 - PP_0710 0.43 -1.7 826,375 - PP_0710 0.43 -1.3 826,375 - PP_0710 0.43 -0.7 826,410 - PP_0710 0.52 +0.8 826,447 + PP_0710 0.61 +0.2 826,777 + +0.1 826,778 - +0.2 826,853 + +2.5 826,955 - PP_0711 0.23 -0.3 827,160 - PP_0711 0.56 -0.2 827,160 - PP_0711 0.56 +0.3 827,160 - PP_0711 0.56 -0.3 827,160 - PP_0711 0.56 +0.4 827,380 - +0.6 827,410 + -0.5 827,410 + +0.2 827,428 + +2.1 827,429 - -1.2 827,495 - +0.3 827,495 - -0.3 827,504 + -0.2 827,504 + -0.9 827,504 + +2.1 827,504 + +0.7 827,518 + -0.4
Or see this region's nucleotide sequence