Strain Fitness in Pseudomonas putida KT2440 around PP_0351

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0350 and PP_0351 are separated by 251 nucleotidesPP_0351 and PP_0352 overlap by 4 nucleotidesPP_0352 and PP_0353 are separated by 147 nucleotides PP_0350: PP_0350 - Outer membrane ferrichrome-iron receptor, at 425,366 to 427,798 _0350 PP_0351: PP_0351 - putative Transmembrane sensor protein, at 428,050 to 429,006 _0351 PP_0352: PP_0352 - RNA polymerase sigma-70 factor, ECF subfamily, at 429,003 to 429,521 _0352 PP_0353: PP_0353 - Exonuclease, at 429,669 to 430,379 _0353 Position (kb) 428 429 430Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 427.134 kb on + strand, within PP_0350at 427.134 kb on + strand, within PP_0350at 427.134 kb on + strand, within PP_0350at 427.134 kb on + strand, within PP_0350at 427.134 kb on + strand, within PP_0350at 427.134 kb on + strand, within PP_0350at 427.135 kb on - strand, within PP_0350at 427.213 kb on + strand, within PP_0350at 427.249 kb on + strand, within PP_0350at 427.251 kb on + strand, within PP_0350at 427.251 kb on + strand, within PP_0350at 427.251 kb on + strand, within PP_0350at 427.251 kb on + strand, within PP_0350at 427.252 kb on - strand, within PP_0350at 427.252 kb on - strand, within PP_0350at 427.383 kb on + strand, within PP_0350at 427.383 kb on + strand, within PP_0350at 427.384 kb on - strand, within PP_0350at 427.482 kb on + strand, within PP_0350at 427.482 kb on + strand, within PP_0350at 427.482 kb on + strand, within PP_0350at 427.483 kb on - strand, within PP_0350at 427.558 kb on - strandat 427.558 kb on - strandat 427.680 kb on + strandat 427.680 kb on + strandat 427.681 kb on - strandat 427.777 kb on + strandat 427.777 kb on + strandat 427.777 kb on + strandat 427.778 kb on - strandat 427.778 kb on - strandat 427.973 kb on + strandat 427.973 kb on + strandat 427.996 kb on - strandat 428.051 kb on + strandat 428.051 kb on + strandat 428.082 kb on + strandat 428.082 kb on + strandat 428.083 kb on - strandat 428.083 kb on - strandat 428.248 kb on + strand, within PP_0351at 428.248 kb on + strand, within PP_0351at 428.248 kb on + strand, within PP_0351at 428.249 kb on - strand, within PP_0351at 428.249 kb on - strand, within PP_0351at 428.249 kb on - strand, within PP_0351at 428.425 kb on - strand, within PP_0351at 428.492 kb on - strand, within PP_0351at 428.577 kb on - strand, within PP_0351at 428.645 kb on - strand, within PP_0351at 428.645 kb on - strand, within PP_0351at 428.645 kb on - strand, within PP_0351at 428.682 kb on - strand, within PP_0351at 428.682 kb on - strand, within PP_0351at 429.031 kb on + strandat 429.031 kb on + strandat 429.031 kb on + strandat 429.032 kb on - strandat 429.032 kb on - strandat 429.032 kb on - strandat 429.058 kb on + strand, within PP_0352at 429.059 kb on - strand, within PP_0352at 429.268 kb on + strand, within PP_0352at 429.268 kb on + strand, within PP_0352at 429.268 kb on + strand, within PP_0352at 429.476 kb on - strandat 429.641 kb on + strandat 429.642 kb on - strandat 429.664 kb on + strandat 429.665 kb on - strandat 429.670 kb on + strandat 429.670 kb on + strandat 429.671 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
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427,134 + PP_0350 0.73 +0.4
427,134 + PP_0350 0.73 +0.6
427,134 + PP_0350 0.73 -1.8
427,134 + PP_0350 0.73 +0.5
427,134 + PP_0350 0.73 -0.3
427,134 + PP_0350 0.73 -0.3
427,135 - PP_0350 0.73 -0.6
427,213 + PP_0350 0.76 -0.3
427,249 + PP_0350 0.77 +0.6
427,251 + PP_0350 0.77 +0.8
427,251 + PP_0350 0.77 -0.4
427,251 + PP_0350 0.77 +0.1
427,251 + PP_0350 0.77 +0.2
427,252 - PP_0350 0.78 +0.0
427,252 - PP_0350 0.78 +0.3
427,383 + PP_0350 0.83 -0.8
427,383 + PP_0350 0.83 -0.1
427,384 - PP_0350 0.83 +0.2
427,482 + PP_0350 0.87 +0.5
427,482 + PP_0350 0.87 +0.4
427,482 + PP_0350 0.87 -0.2
427,483 - PP_0350 0.87 +0.6
427,558 - -0.4
427,558 - +0.5
427,680 + -0.5
427,680 + -0.2
427,681 - -0.4
427,777 + +0.4
427,777 + -0.1
427,777 + +1.1
427,778 - +0.8
427,778 - -0.1
427,973 + +2.1
427,973 + +1.4
427,996 - -0.1
428,051 + -0.2
428,051 + +0.4
428,082 + -0.0
428,082 + -1.0
428,083 - -2.8
428,083 - -2.8
428,248 + PP_0351 0.21 -0.4
428,248 + PP_0351 0.21 -0.4
428,248 + PP_0351 0.21 -0.9
428,249 - PP_0351 0.21 -4.0
428,249 - PP_0351 0.21 -1.8
428,249 - PP_0351 0.21 +0.5
428,425 - PP_0351 0.39 -2.3
428,492 - PP_0351 0.46 -1.5
428,577 - PP_0351 0.55 -0.8
428,645 - PP_0351 0.62 -3.0
428,645 - PP_0351 0.62 -2.0
428,645 - PP_0351 0.62 -2.3
428,682 - PP_0351 0.66 -1.1
428,682 - PP_0351 0.66 +0.0
429,031 + -0.3
429,031 + +1.1
429,031 + -0.4
429,032 - -0.5
429,032 - -1.5
429,032 - +0.7
429,058 + PP_0352 0.11 +0.4
429,059 - PP_0352 0.11 +1.8
429,268 + PP_0352 0.51 -1.4
429,268 + PP_0352 0.51 -0.8
429,268 + PP_0352 0.51 -0.2
429,476 - +2.1
429,641 + +0.5
429,642 - -1.2
429,664 + +1.1
429,665 - +1.1
429,670 + +1.1
429,670 + +0.0
429,671 - -0.6

Or see this region's nucleotide sequence