Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0136 and PP_0137 are separated by 745 nucleotides PP_0137 and PP_0138 are separated by 147 nucleotides
PP_0136: PP_0136 - conserved exported protein of unknown function, at 143,551 to 144,021
_0136
PP_0137: PP_0137 - glutamate/aspartate-proton DAACS transporter, at 144,767 to 146,095
_0137
PP_0138: PP_0138 - conserved membrane protein of unknown function, at 146,243 to 147,472
_0138
Position (kb) 144
145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 143.788 kb on + strand, within PP_0136 at 143.788 kb on + strand, within PP_0136 at 143.788 kb on + strand, within PP_0136 at 143.788 kb on + strand, within PP_0136 at 143.788 kb on + strand, within PP_0136 at 143.789 kb on - strand, within PP_0136 at 143.789 kb on - strand, within PP_0136 at 143.789 kb on - strand, within PP_0136 at 143.789 kb on - strand, within PP_0136 at 143.789 kb on - strand, within PP_0136 at 143.789 kb on - strand, within PP_0136 at 143.831 kb on - strand, within PP_0136 at 143.831 kb on - strand, within PP_0136 at 143.831 kb on - strand, within PP_0136 at 143.831 kb on - strand, within PP_0136 at 143.875 kb on + strand, within PP_0136 at 144.017 kb on - strand at 144.064 kb on + strand at 144.065 kb on - strand at 144.065 kb on - strand at 144.067 kb on + strand at 144.067 kb on + strand at 144.068 kb on - strand at 144.068 kb on - strand at 144.068 kb on - strand at 144.085 kb on - strand at 144.086 kb on + strand at 144.086 kb on + strand at 144.087 kb on - strand at 144.099 kb on + strand at 144.099 kb on + strand at 144.099 kb on + strand at 144.099 kb on + strand at 144.099 kb on + strand at 144.100 kb on - strand at 144.100 kb on - strand at 144.100 kb on - strand at 144.160 kb on + strand at 144.161 kb on - strand at 144.178 kb on + strand at 144.178 kb on + strand at 144.179 kb on - strand at 144.202 kb on + strand at 144.203 kb on - strand at 144.262 kb on - strand at 144.294 kb on + strand at 144.295 kb on - strand at 144.295 kb on - strand at 144.295 kb on - strand at 144.295 kb on - strand at 144.352 kb on + strand at 144.352 kb on + strand at 144.353 kb on - strand at 144.386 kb on + strand at 144.386 kb on + strand at 144.386 kb on + strand at 144.386 kb on + strand at 144.387 kb on - strand at 144.439 kb on + strand at 144.439 kb on + strand at 144.503 kb on - strand at 144.545 kb on - strand at 144.581 kb on - strand at 144.605 kb on + strand at 144.605 kb on + strand at 144.605 kb on + strand at 144.606 kb on - strand at 144.606 kb on - strand at 144.606 kb on - strand at 144.660 kb on + strand at 144.660 kb on + strand at 144.660 kb on + strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.661 kb on - strand at 144.688 kb on + strand at 144.688 kb on - strand at 144.688 kb on - strand at 144.688 kb on - strand at 144.760 kb on + strand at 144.760 kb on + strand at 145.025 kb on + strand, within PP_0137 at 145.025 kb on + strand, within PP_0137 at 145.026 kb on - strand, within PP_0137 at 145.026 kb on - strand, within PP_0137 at 145.059 kb on + strand, within PP_0137 at 145.059 kb on + strand, within PP_0137 at 145.129 kb on + strand, within PP_0137 at 145.129 kb on + strand, within PP_0137 at 145.139 kb on + strand, within PP_0137 at 145.139 kb on + strand, within PP_0137 at 145.162 kb on - strand, within PP_0137 at 145.162 kb on - strand, within PP_0137 at 145.324 kb on + strand, within PP_0137 at 145.380 kb on - strand, within PP_0137 at 145.380 kb on - strand, within PP_0137 at 145.380 kb on - strand, within PP_0137 at 145.475 kb on + strand, within PP_0137 at 145.475 kb on + strand, within PP_0137 at 145.475 kb on + strand, within PP_0137 at 145.476 kb on - strand, within PP_0137 at 145.476 kb on - strand, within PP_0137 at 145.506 kb on + strand, within PP_0137 at 145.586 kb on + strand, within PP_0137 at 145.586 kb on + strand, within PP_0137 at 145.643 kb on + strand, within PP_0137 at 145.701 kb on + strand, within PP_0137 at 145.702 kb on - strand, within PP_0137 at 145.718 kb on + strand, within PP_0137 at 145.719 kb on - strand, within PP_0137 at 145.754 kb on + strand, within PP_0137 at 145.754 kb on + strand, within PP_0137 at 145.842 kb on + strand, within PP_0137 at 145.842 kb on + strand, within PP_0137 at 145.943 kb on - strand, within PP_0137 at 145.943 kb on - strand, within PP_0137 at 146.006 kb on + strand at 146.006 kb on + strand at 146.006 kb on + strand at 146.007 kb on - strand at 146.007 kb on - strand at 146.007 kb on - strand at 146.030 kb on + strand at 146.031 kb on - strand at 146.031 kb on - strand at 146.033 kb on + strand at 146.034 kb on - strand at 146.034 kb on - strand at 146.067 kb on + strand at 146.067 kb on + strand at 146.204 kb on + strand at 146.238 kb on + strand at 146.238 kb on + strand at 146.238 kb on + strand at 146.238 kb on + strand at 146.238 kb on + strand at 146.239 kb on - strand at 146.239 kb on - strand at 146.239 kb on - strand at 146.239 kb on - strand at 146.239 kb on - strand at 146.239 kb on - strand at 146.239 kb on - strand at 146.585 kb on - strand, within PP_0138 at 146.721 kb on - strand, within PP_0138 at 146.861 kb on + strand, within PP_0138 at 146.861 kb on + strand, within PP_0138 at 146.861 kb on + strand, within PP_0138 at 146.862 kb on - strand, within PP_0138 at 146.862 kb on - strand at 146.862 kb on - strand, within PP_0138 at 147.023 kb on + strand, within PP_0138 at 147.023 kb on + strand, within PP_0138 at 147.023 kb on + strand, within PP_0138 at 147.023 kb on + strand, within PP_0138 at 147.023 kb on + strand, within PP_0138 at 147.023 kb on + strand, within PP_0138 at 147.024 kb on - strand, within PP_0138 at 147.024 kb on - strand, within PP_0138 at 147.024 kb on - strand, within PP_0138 at 147.024 kb on - strand, within PP_0138 at 147.083 kb on + strand, within PP_0138 at 147.083 kb on + strand, within PP_0138
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 143,788 + PP_0136 0.50 -1.4 143,788 + PP_0136 0.50 -0.5 143,788 + PP_0136 0.50 -0.6 143,788 + PP_0136 0.50 +0.4 143,788 + PP_0136 0.50 +0.9 143,789 - PP_0136 0.51 +0.2 143,789 - PP_0136 0.51 -0.4 143,789 - PP_0136 0.51 +0.6 143,789 - PP_0136 0.51 +1.2 143,789 - PP_0136 0.51 +0.0 143,789 - PP_0136 0.51 -1.6 143,831 - PP_0136 0.59 +0.4 143,831 - PP_0136 0.59 +1.3 143,831 - PP_0136 0.59 +1.4 143,831 - PP_0136 0.59 -1.2 143,875 + PP_0136 0.69 +1.0 144,017 - +1.2 144,064 + +3.4 144,065 - -0.7 144,065 - +0.0 144,067 + -1.0 144,067 + -0.9 144,068 - -1.2 144,068 - -0.5 144,068 - +0.4 144,085 - -0.0 144,086 + -0.4 144,086 + -0.6 144,087 - -0.1 144,099 + +1.0 144,099 + -1.5 144,099 + +2.0 144,099 + -0.1 144,099 + -1.2 144,100 - +0.6 144,100 - -0.7 144,100 - -0.3 144,160 + -0.2 144,161 - -1.1 144,178 + -0.6 144,178 + +0.3 144,179 - +0.1 144,202 + +0.1 144,203 - +0.3 144,262 - -0.7 144,294 + -0.3 144,295 - +2.0 144,295 - -1.1 144,295 - +0.2 144,295 - -0.5 144,352 + +0.1 144,352 + -0.1 144,353 - -0.4 144,386 + -0.5 144,386 + -0.7 144,386 + -0.8 144,386 + +0.7 144,387 - -1.7 144,439 + -0.1 144,439 + -0.3 144,503 - +0.4 144,545 - +0.7 144,581 - -1.6 144,605 + -0.4 144,605 + -0.3 144,605 + +0.4 144,606 - -0.3 144,606 - -0.4 144,606 - -0.8 144,660 + -2.7 144,660 + -0.2 144,660 + -1.0 144,661 - -0.2 144,661 - +0.7 144,661 - -0.5 144,661 - +0.6 144,661 - +0.4 144,661 - +0.4 144,661 - -0.1 144,661 - +1.5 144,688 + -0.3 144,688 - +0.1 144,688 - +0.7 144,688 - -0.8 144,760 + +0.1 144,760 + +0.8 145,025 + PP_0137 0.19 -1.0 145,025 + PP_0137 0.19 -0.6 145,026 - PP_0137 0.19 -0.1 145,026 - PP_0137 0.19 +0.6 145,059 + PP_0137 0.22 -0.7 145,059 + PP_0137 0.22 +0.2 145,129 + PP_0137 0.27 +0.1 145,129 + PP_0137 0.27 -1.7 145,139 + PP_0137 0.28 -1.0 145,139 + PP_0137 0.28 -0.6 145,162 - PP_0137 0.30 +2.0 145,162 - PP_0137 0.30 +0.2 145,324 + PP_0137 0.42 -0.4 145,380 - PP_0137 0.46 +0.3 145,380 - PP_0137 0.46 -1.1 145,380 - PP_0137 0.46 +0.2 145,475 + PP_0137 0.53 -0.1 145,475 + PP_0137 0.53 +0.1 145,475 + PP_0137 0.53 +0.0 145,476 - PP_0137 0.53 +0.6 145,476 - PP_0137 0.53 -0.2 145,506 + PP_0137 0.56 -1.1 145,586 + PP_0137 0.62 -0.4 145,586 + PP_0137 0.62 -0.2 145,643 + PP_0137 0.66 +0.1 145,701 + PP_0137 0.70 +0.6 145,702 - PP_0137 0.70 +0.1 145,718 + PP_0137 0.72 +1.0 145,719 - PP_0137 0.72 +0.9 145,754 + PP_0137 0.74 +0.6 145,754 + PP_0137 0.74 -0.8 145,842 + PP_0137 0.81 +0.3 145,842 + PP_0137 0.81 -0.6 145,943 - PP_0137 0.88 +0.2 145,943 - PP_0137 0.88 +0.9 146,006 + +0.8 146,006 + -1.2 146,006 + -0.1 146,007 - -2.5 146,007 - -0.7 146,007 - -0.2 146,030 + -0.6 146,031 - -0.2 146,031 - +0.6 146,033 + +0.6 146,034 - -0.3 146,034 - -1.1 146,067 + +0.4 146,067 + -0.7 146,204 + +1.0 146,238 + -0.2 146,238 + -0.5 146,238 + -0.3 146,238 + +2.0 146,238 + -1.0 146,239 - -0.2 146,239 - -0.6 146,239 - -1.1 146,239 - +1.0 146,239 - +0.3 146,239 - -0.2 146,239 - +0.9 146,585 - PP_0138 0.28 -0.5 146,721 - PP_0138 0.39 -1.3 146,861 + PP_0138 0.50 -0.4 146,861 + PP_0138 0.50 +0.6 146,861 + PP_0138 0.50 -0.8 146,862 - PP_0138 0.50 +0.2 146,862 - +2.0 146,862 - PP_0138 0.50 -1.1 147,023 + PP_0138 0.63 -0.3 147,023 + PP_0138 0.63 +0.3 147,023 + PP_0138 0.63 +0.5 147,023 + PP_0138 0.63 +2.0 147,023 + PP_0138 0.63 -0.2 147,023 + PP_0138 0.63 +0.3 147,024 - PP_0138 0.63 -0.3 147,024 - PP_0138 0.63 -0.1 147,024 - PP_0138 0.63 -0.4 147,024 - PP_0138 0.63 -0.8 147,083 + PP_0138 0.68 +1.3 147,083 + PP_0138 0.68 +0.0
Or see this region's nucleotide sequence