Strain Fitness in Pseudomonas putida KT2440 around PP_5489

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
2,182,359	+		PP_5487	0.43	-1.9
2,182,408	+		PP_5487	0.55	+0.3
2,182,413	-		PP_5487	0.57	-0.6
2,182,446	+		PP_5487	0.65	+2.8
2,182,580	+				-1.3
2,182,698	+		PP_5488	0.15	+0.6
2,182,743	+		PP_5488	0.22	-0.8
2,182,743	+		PP_5488	0.22	-0.5
2,182,909	-		PP_5488	0.47	-0.9
2,182,938	+		PP_5488	0.52	-0.7
2,182,938	+		PP_5488	0.52	+0.2
2,182,940	+		PP_5488	0.52	-0.2
2,183,189	+		PP_5488	0.90	-1.0
2,183,191	+				-0.4
2,183,192	-				-1.2
2,183,192	-				-0.5
2,183,195	+				-0.6
2,183,253	+				+0.2
2,183,254	-				-0.1
2,183,258	+				-1.0
2,183,259	-				-0.4
2,183,507	-		PP_5489	0.47	-0.1
2,183,535	+		PP_5489	0.53	-2.4
2,183,536	-		PP_5489	0.53	-1.5
2,183,592	+		PP_5489	0.64	+1.0
2,183,597	+		PP_5489	0.65	-0.8
2,183,597	+		PP_5489	0.65	-1.0
2,183,743	-				-0.4
2,183,789	-				+2.2
2,183,828	-				+1.2
2,183,942	+				+0.9
2,183,992	+				-1.6
2,184,004	+		PP_5490	0.11	-0.6
2,184,167	+		PP_5490	0.40	+0.6
2,184,167	+		PP_5490	0.40	+1.2
2,184,168	-		PP_5490	0.40	-0.8
2,184,236	+		PP_5490	0.52	-1.0
2,184,237	-		PP_5490	0.52	+0.7
2,184,256	+		PP_5490	0.55	-1.3
2,184,257	-		PP_5490	0.55	-1.0
2,184,265	+		PP_5490	0.57	-0.1
2,184,350	-		PP_5490	0.72	-1.6
2,184,377	+		PP_5490	0.77	+0.1
2,184,412	+		PP_5490	0.83	+0.4
2,184,567	+				-0.3
2,184,567	+				-0.1
2,184,568	-				+0.2
2,184,568	-				-0.2
2,184,628	+				-1.6
2,184,628	+				+0.7
2,184,628	+				+1.0
2,184,628	+				+0.4
2,184,658	-				+0.4
2,184,739	+				-1.3
2,184,739	+				+0.7
2,184,751	+				+0.4
2,184,752	-				+0.2
2,184,752	-				+0.0
2,184,763	-				-1.0