Strain Fitness in Pseudomonas putida KT2440 around PP_5356

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
6,105,836	-		PP_5355	0.65	-1.0
6,105,836	-		PP_5355	0.65	+1.8
6,105,966	+		PP_5355	0.72	-0.3
6,106,355	+				-1.4
6,106,356	-				+0.1
6,106,356	-				-1.6
6,106,356	-				+0.5
6,106,466	+				+0.0
6,106,466	+				-0.8
6,106,467	-				+0.1
6,106,467	-				-0.0
6,106,467	-				-0.8
6,106,539	+				+0.4
6,106,539	+				+0.0
6,106,539	+				-0.5
6,106,540	-				+0.1
6,106,540	-				+0.7
6,106,540	-				-0.5
6,106,543	+				-2.6
6,106,543	+				+3.7
6,106,543	+				+1.4
6,106,543	+				-1.3
6,106,543	+				-0.1
6,106,543	+				+0.7
6,106,544	-				-1.1
6,106,544	-				+0.3
6,106,548	+				+0.4
6,106,549	-				-0.1
6,106,549	-				+1.7
6,106,582	+				+0.5
6,106,583	-				+0.1
6,106,583	-				-1.3
6,106,583	-				+0.8
6,106,583	-				-0.1
6,106,583	-				+0.5
6,106,583	-				-0.8
6,106,625	+				+1.9
6,106,626	-				-0.7
6,106,626	-				-0.1
6,106,750	-				+0.3
6,106,798	-				-0.4
6,106,798	-				+0.7
6,106,798	-				+0.1
6,106,871	+		PP_5356	0.15	-0.7
6,106,871	+		PP_5356	0.15	+0.5
6,106,871	+		PP_5356	0.15	+0.1
6,106,871	+		PP_5356	0.15	+0.7
6,106,871	+		PP_5356	0.15	+1.1
6,106,872	-		PP_5356	0.15	-0.1
6,106,872	-		PP_5356	0.15	+0.2
6,106,872	-		PP_5356	0.15	-1.1
6,106,872	-		PP_5356	0.15	+1.4
6,106,872	-		PP_5356	0.15	+0.9
6,106,872	-		PP_5356	0.15	-0.7
6,106,872	-		PP_5356	0.15	+0.4
6,106,872	-		PP_5356	0.15	+0.6
6,106,895	-		PP_5356	0.20	+0.1
6,106,895	-		PP_5356	0.20	+1.1
6,106,912	+		PP_5356	0.23	-0.8
6,106,912	+		PP_5356	0.23	+0.2
6,106,912	+		PP_5356	0.23	+0.8
6,106,912	+		PP_5356	0.23	+0.7
6,106,912	+		PP_5356	0.23	-1.4
6,106,912	+		PP_5356	0.23	+2.3
6,106,912	+		PP_5356	0.23	+0.1
6,106,913	-		PP_5356	0.24	+0.9
6,106,913	-		PP_5356	0.24	+0.6
6,106,913	-		PP_5356	0.24	-0.1
6,106,913	-		PP_5356	0.24	+0.7
6,106,913	-		PP_5356	0.24	-0.1
6,106,913	-		PP_5356	0.24	+0.5
6,106,942	+		PP_5356	0.30	-0.9
6,106,942	+		PP_5356	0.30	-0.6
6,106,943	-		PP_5356	0.30	-1.7
6,106,943	-		PP_5356	0.30	+1.1
6,106,943	-		PP_5356	0.30	-0.3
6,107,281	+				+1.0
6,107,281	+				+1.3
6,107,292	+				-1.6
6,107,359	+				+0.4
6,107,360	-				+1.7
6,107,360	-				+0.4
6,107,360	-				-0.5
6,107,490	+		PP_5357	0.24	-0.8
6,107,610	+		PP_5357	0.38	+0.2
6,107,611	-		PP_5357	0.38	+0.3
6,107,611	-		PP_5357	0.38	-0.2
6,107,821	+		PP_5357	0.62	-0.7
6,107,821	+		PP_5357	0.62	+0.4
6,107,821	+		PP_5357	0.62	+0.2
6,107,822	-		PP_5357	0.62	-0.1
6,107,822	-		PP_5357	0.62	+0.9
6,107,822	-		PP_5357	0.62	+0.8
6,107,822	-		PP_5357	0.62	+2.4
6,107,822	-		PP_5357	0.62	-0.1
6,107,822	-		PP_5357	0.62	+0.1
6,107,823	+		PP_5357	0.62	-0.3
6,107,823	+		PP_5357	0.62	-0.6
6,107,823	+		PP_5357	0.62	-1.5
6,107,824	-		PP_5357	0.62	+2.2
6,108,007	+		PP_5357	0.83	-0.2
6,108,008	-		PP_5357	0.83	+0.9
6,108,036	-		PP_5357	0.87	+0.6
6,108,143	+				-0.6
6,108,177	+				-2.3
6,108,178	-				+1.2
6,108,198	-				+0.9
6,108,198	-				-0.1
6,108,212	+				+0.1
6,108,213	-				-0.1
6,108,213	-				+0.7
6,108,213	-				+1.1
6,108,213	-				+2.2
6,108,213	-				-0.3
6,108,218	+				-2.0
6,108,219	-				+0.5
6,108,252	-				+0.2
6,108,252	-				+1.5
6,108,266	+				-0.1
6,108,266	+				+0.8
6,108,266	+				+0.3
6,108,266	+				-0.2
6,108,266	+				-0.8
6,108,267	-				+1.8
6,108,267	-				+0.4
6,108,267	-				+0.1
6,108,267	-				+1.5
6,108,267	-				+0.7