Strain Fitness in Pseudomonas putida KT2440 around PP_5352

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
6,099,855	+		PP_5351	0.47	+0.1
6,099,855	+		PP_5351	0.47	-0.9
6,099,856	-		PP_5351	0.47	+0.5
6,099,856	-		PP_5351	0.47	+1.5
6,099,856	-		PP_5351	0.47	-0.3
6,099,859	-		PP_5351	0.47	-0.2
6,099,876	-		PP_5351	0.48	+0.4
6,099,962	-				+1.7
6,100,044	+		PP_5351	0.59	+0.7
6,100,083	+		PP_5351	0.62	-0.5
6,100,084	-		PP_5351	0.62	+0.3
6,100,086	+		PP_5351	0.62	-0.6
6,100,086	+		PP_5351	0.62	+0.5
6,100,086	+		PP_5351	0.62	+0.4
6,100,087	-		PP_5351	0.62	-0.3
6,100,087	-		PP_5351	0.62	+0.6
6,100,182	+		PP_5351	0.69	-0.7
6,100,182	+		PP_5351	0.69	+1.7
6,100,183	-		PP_5351	0.69	-0.1
6,100,291	+		PP_5351	0.76	-0.1
6,100,291	+		PP_5351	0.76	-0.8
6,100,291	+		PP_5351	0.76	-0.1
6,100,291	+		PP_5351	0.76	+0.8
6,100,382	+		PP_5351	0.83	+0.9
6,100,383	-		PP_5351	0.83	+0.7
6,100,425	+		PP_5351	0.86	-0.2
6,100,426	-		PP_5351	0.86	+1.7
6,100,510	+				-1.0
6,100,511	-				+0.1
6,100,668	+				-0.2
6,100,668	+				-0.6
6,100,797	-				-0.4
6,100,802	+				-0.2
6,100,803	-				+0.4
6,100,807	+				-0.8
6,100,811	+				-1.4
6,100,837	+				-0.6
6,101,331	+		PP_5352	0.22	+3.3
6,101,377	+		PP_5352	0.24	+2.7
6,101,377	+		PP_5352	0.24	+2.7
6,101,377	+		PP_5352	0.24	+4.3
6,101,377	+		PP_5352	0.24	+3.1
6,101,377	+		PP_5352	0.24	+3.7
6,101,377	+		PP_5352	0.24	+1.7
6,101,377	+		PP_5352	0.24	+1.7
6,101,378	-		PP_5352	0.24	+1.7
6,101,378	-		PP_5352	0.24	+1.7
6,101,378	-		PP_5352	0.24	+2.7
6,101,379	+		PP_5352	0.24	+2.7
6,101,485	+		PP_5352	0.29	+2.7
6,101,485	+		PP_5352	0.29	+1.1
6,101,486	-		PP_5352	0.29	+1.7
6,101,529	+		PP_5352	0.31	+1.1
6,101,617	+		PP_5352	0.35	+1.1
6,101,617	+		PP_5352	0.35	+2.7
6,101,618	-		PP_5352	0.35	+1.7
6,101,618	-		PP_5352	0.35	+2.7
6,101,618	-		PP_5352	0.35	+0.4
6,101,656	+		PP_5352	0.37	+1.7
6,101,704	+		PP_5352	0.39	+1.1
6,101,704	+		PP_5352	0.39	+0.7
6,101,704	-		PP_5352	0.39	+2.7
6,101,827	+		PP_5352	0.45	+1.7
6,101,827	+		PP_5352	0.45	+2.7
6,101,828	-		PP_5352	0.45	-0.5
6,101,828	-		PP_5352	0.45	+1.1
6,101,851	+		PP_5352	0.46	+1.7
6,101,851	+		PP_5352	0.46	-0.5
6,101,851	+		PP_5352	0.46	+1.7
6,101,852	-		PP_5352	0.46	-0.5
6,101,852	-		PP_5352	0.46	+3.7
6,101,918	-		PP_5352	0.49	+1.1
6,101,918	-		PP_5352	0.49	+0.1
6,101,986	+		PP_5352	0.52	+0.8
6,101,986	+		PP_5352	0.52	+2.7
6,101,986	+		PP_5352	0.52	+2.7
6,101,987	-		PP_5352	0.52	+2.0
6,102,037	+		PP_5352	0.54	+2.7
6,102,038	-		PP_5352	0.54	+2.7
6,102,038	-		PP_5352	0.54	+1.7
6,102,291	+		PP_5352	0.66	+2.1
6,102,291	+		PP_5352	0.66	+1.7
6,102,291	+		PP_5352	0.66	-0.3
6,102,292	-		PP_5352	0.66	+1.3
6,102,334	+		PP_5352	0.68	+2.7
6,102,334	+		PP_5352	0.68	+2.1
6,102,335	-		PP_5352	0.68	-0.9
6,102,335	-		PP_5352	0.68	+0.5
6,102,374	+		PP_5352	0.70	+2.7
6,102,489	+		PP_5352	0.75	-1.6
6,102,490	-		PP_5352	0.75	+1.1
6,102,562	+		PP_5352	0.78	+0.4
6,102,562	+		PP_5352	0.78	+1.4
6,102,562	+		PP_5352	0.78	+2.7
6,102,562	+		PP_5352	0.78	+0.4
6,102,562	+		PP_5352	0.78	+0.1
6,102,563	-		PP_5352	0.78	+2.7
6,102,563	-		PP_5352	0.78	+1.7
6,102,563	-		PP_5352	0.78	+0.7
6,102,563	-		PP_5352	0.78	+1.1
6,102,563	-		PP_5352	0.78	+1.7
6,102,655	+		PP_5352	0.83	+2.7
6,102,655	+		PP_5352	0.83	+2.7
6,102,655	+		PP_5352	0.83	+2.7
6,102,655	+		PP_5352	0.83	+1.1
6,102,655	+		PP_5352	0.83	+2.7
6,102,655	+		PP_5352	0.83	+1.7
6,102,656	-		PP_5352	0.83	+1.7
6,102,656	-		PP_5352	0.83	+2.7
6,102,656	-		PP_5352	0.83	+0.7
6,102,656	-		PP_5352	0.83	+2.7
6,102,656	-		PP_5352	0.83	+0.1
6,102,656	-		PP_5352	0.83	+0.4
6,102,656	-		PP_5352	0.83	+0.4
6,102,656	-		PP_5352	0.83	+0.4
6,102,659	+		PP_5352	0.83	+1.7
6,102,659	+		PP_5352	0.83	+0.7
6,102,659	+		PP_5352	0.83	+0.7
6,102,659	+		PP_5352	0.83	+0.7
6,102,659	+		PP_5352	0.83	+1.1
6,102,659	+		PP_5352	0.83	+2.7
6,102,660	-		PP_5352	0.83	+1.1
6,102,660	-		PP_5352	0.83	+2.7
6,102,660	-		PP_5352	0.83	+2.7
6,102,660	-		PP_5352	0.83	+0.1
6,102,660	-		PP_5352	0.83	+0.7
6,102,660	-		PP_5352	0.83	+2.7
6,102,660	-		PP_5352	0.83	+1.7
6,102,660	-		PP_5352	0.83	+0.4
6,102,660	-		PP_5352	0.83	+0.7
6,102,660	-		PP_5352	0.83	+3.7
6,102,660	-		PP_5352	0.83	+0.1
6,102,697	+		PP_5352	0.84	+0.4
6,102,697	+		PP_5352	0.84	+1.1
6,102,697	+		PP_5352	0.84	+0.7
6,102,698	-		PP_5352	0.85	+1.7
6,102,698	-		PP_5352	0.85	+1.1
6,102,718	+		PP_5352	0.85	+2.7
6,102,733	+		PP_5352	0.86	-1.6
6,102,734	-		PP_5352	0.86	+2.7
6,102,788	+		PP_5352	0.89	-0.4
6,102,788	+		PP_5352	0.89	-0.7
6,102,788	+		PP_5352	0.89	-0.5
6,102,788	+		PP_5352	0.89	-0.5
6,102,788	+		PP_5352	0.89	-1.9
6,102,789	-		PP_5352	0.89	-0.3
6,102,789	-		PP_5352	0.89	+0.1
6,102,789	-		PP_5352	0.89	-1.3
6,102,914	+				-1.1
6,102,990	+				+0.7
6,102,990	+				+0.4
6,103,033	+				-0.9
6,103,066	+				-1.0
6,103,067	-				+0.7
6,103,093	+				-0.5
6,103,093	+				-1.5
6,103,094	-				-0.5
6,103,094	-				+1.3
6,103,094	-				-0.5
6,103,167	+				+0.3
6,103,167	+				-0.8
6,103,167	+				+0.4
6,103,167	+				+0.7
6,103,167	+				+0.6
6,103,167	+				+0.5
6,103,167	+				+0.5
6,103,168	-				-0.4
6,103,168	-				+0.5
6,103,168	-				-0.9
6,103,168	-				+0.5
6,103,168	-				+0.2
6,103,168	-				+1.1
6,103,168	-				-0.4
6,103,168	-				-0.8
6,103,168	-				+0.4
6,103,168	-				-0.9
6,103,168	-				+0.9
6,103,168	-				-0.0
6,103,168	-				+0.1
6,103,168	-				+1.6
6,103,182	+				-0.3
6,103,276	-		PP_5353	0.13	+0.3
6,103,328	+		PP_5353	0.19	+0.4
6,103,821	+		PP_5353	0.76	+1.1
6,103,821	+		PP_5353	0.76	-0.4
6,103,821	+		PP_5353	0.76	+1.0
6,103,821	+		PP_5353	0.76	+0.6
6,103,821	+		PP_5353	0.76	+2.7
6,103,821	+		PP_5353	0.76	+0.3
6,103,821	+		PP_5353	0.76	+1.3
6,103,822	-		PP_5353	0.76	-0.1
6,103,822	-		PP_5353	0.76	+2.3
6,103,825	+		PP_5353	0.77	-0.9
6,103,825	+		PP_5353	0.77	-0.3
6,103,825	+		PP_5353	0.77	+1.7
6,103,826	-		PP_5353	0.77	-0.9
6,103,826	-		PP_5353	0.77	+0.2
6,104,020	+				-1.2
6,104,034	+				+0.6
6,104,034	+				+0.0
6,104,034	+				+0.5
6,104,035	-				-0.8