Strain Fitness in Pseudomonas putida KT2440 around PP_5158

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
5,883,183	-				+0.1
5,883,215	+				+0.7
5,883,215	+				-0.3
5,883,215	+				+0.9
5,883,258	+				+0.5
5,883,259	-				+0.4
5,883,259	-				+1.7
5,883,268	-				+0.6
5,883,268	-				-1.6
5,883,268	-				-0.2
5,883,302	-				-2.5
5,883,342	+				-0.1
5,883,343	-				+3.1
5,883,414	+				-0.1
5,883,414	+				+0.4
5,883,414	+				-1.4
5,883,415	-				+0.1
5,883,415	-				-0.1
5,883,415	-				+0.7
5,883,415	-				-2.4
5,883,415	-				+0.4
5,883,415	-				-0.9
5,883,431	-				+0.2
5,883,431	-				-0.2
5,883,436	+				-1.0
5,883,437	-				-0.1
5,883,437	-				-0.4
5,883,448	+				-0.3
5,883,448	+				+0.3
5,883,449	-				-0.5
5,883,521	-		PP_5157	0.21	+1.1
5,883,540	+		PP_5157	0.25	-0.1
5,883,678	+		PP_5157	0.53	-0.3
5,883,678	+		PP_5157	0.53	+1.7
5,883,679	-		PP_5157	0.54	-2.2
5,883,736	+		PP_5157	0.65	+0.0
5,883,736	+		PP_5157	0.65	+1.4
5,883,737	-		PP_5157	0.65	-0.1
5,883,737	-		PP_5157	0.65	-0.1
5,883,737	-		PP_5157	0.65	+1.1
5,883,737	-		PP_5157	0.65	-0.6
5,883,737	-		PP_5157	0.65	-1.8
5,883,903	+				-0.5
5,883,904	-				+0.4
5,883,955	-				-1.3
5,883,965	-				-0.6
5,884,034	+				+1.3
5,884,034	+				-0.5
5,884,034	+				-0.5
5,884,034	+				-1.0
5,884,034	+				+0.6
5,884,035	-				-0.7
5,884,035	-				-1.1
5,884,035	-				+1.0
5,884,035	-				-0.3
5,884,035	-				+2.7
5,884,036	+				+0.3
5,884,036	+				-0.0
5,884,036	+				-0.3
5,884,037	-				+1.5
5,884,037	-				+0.1
5,884,037	-				-0.7
5,884,037	-				-0.3
5,884,040	-				+0.6
5,884,161	+		PP_5158	0.27	-0.1
5,884,161	+		PP_5158	0.27	-1.7
5,884,161	+		PP_5158	0.27	-0.2
5,884,161	+		PP_5158	0.27	-0.0
5,884,161	+		PP_5158	0.27	-0.1
5,884,161	+		PP_5158	0.27	+0.2
5,884,161	+		PP_5158	0.27	-1.1
5,884,162	-		PP_5158	0.27	+0.7
5,884,162	-		PP_5158	0.27	-0.1
5,884,162	-		PP_5158	0.27	-1.9
5,884,162	-		PP_5158	0.27	-1.1
5,884,208	+		PP_5158	0.36	-0.6
5,884,382	+		PP_5158	0.71	-2.2
5,884,382	+		PP_5158	0.71	-2.1
5,884,383	-		PP_5158	0.71	-2.1
5,884,383	-		PP_5158	0.71	-0.3
5,884,465	-		PP_5158	0.87	-1.6
5,884,585	-		PP_5159	0.11	+0.3
5,884,614	+		PP_5159	0.16	+1.1
5,884,614	+		PP_5159	0.16	+0.4
5,884,615	-		PP_5159	0.16	-0.2
5,884,717	+		PP_5159	0.32	-0.1
5,884,717	+		PP_5159	0.32	+0.8
5,884,778	-		PP_5159	0.42	-0.3
5,884,795	+		PP_5159	0.45	+0.7
5,884,796	-		PP_5159	0.45	-0.1
5,884,962	-		PP_5159	0.72	+1.4
5,885,131	-				-1.2
5,885,131	-				-0.2
5,885,131	-				+2.0
5,885,150	+				-0.1
5,885,150	+				+0.3
5,885,150	+				+0.3
5,885,150	+				-1.0
5,885,150	+				+1.9
5,885,151	-				+0.9
5,885,203	-				+0.7
5,885,203	-				-1.6
5,885,235	+				+0.6
5,885,235	+				-0.0
5,885,235	+				+0.4
5,885,336	+				-0.8
5,885,336	+				-0.5
5,885,336	+				+0.2
5,885,336	+				+0.1
5,885,336	+				-0.4
5,885,336	+				-0.5
5,885,337	-		PP_5160	0.10	+0.3
5,885,337	-		PP_5160	0.10	+1.0
5,885,337	-		PP_5160	0.10	+2.7
5,885,337	-		PP_5160	0.10	-2.4
5,885,337	-		PP_5160	0.10	+0.0
5,885,338	+		PP_5160	0.10	+0.1
5,885,338	+		PP_5160	0.10	+0.4
5,885,339	-		PP_5160	0.10	+0.0
5,885,339	-		PP_5160	0.10	-1.7
5,885,405	+		PP_5160	0.19	-0.3
5,885,433	-				+0.7