Strain Fitness in Pseudomonas putida KT2440 around PP_5143

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
5,867,775	-				-0.1
5,867,787	-				+0.8
5,867,787	-				-0.3
5,867,789	-				-2.7
5,867,789	-				+0.9
5,868,726	-		PP_5143	0.19	-3.0
5,868,732	-		PP_5143	0.19	-1.7
5,868,732	-		PP_5143	0.19	-2.0
5,868,732	-		PP_5143	0.19	-0.9
5,869,347	-				+1.2
5,869,347	-				-1.3
5,869,347	-				-0.6
5,869,375	-				-1.2
5,869,395	-				-1.0
5,869,419	-				-0.6
5,869,492	+				+2.7
5,869,492	+				+0.9
5,869,492	+				+1.2
5,869,492	+				+0.3
5,869,492	+				-0.4
5,869,493	-				-0.3
5,869,493	-				+0.2
5,869,493	-				+2.7
5,869,493	-				-0.7
5,869,493	-				+0.8
5,869,493	-				+1.0
5,869,493	-				-0.1
5,869,493	-				-1.0
5,869,507	-				-0.2
5,869,569	-		PP_5144	0.10	+0.1
5,869,784	+		PP_5144	0.39	+0.1
5,869,784	+		PP_5144	0.39	+0.3
5,869,785	-		PP_5144	0.39	-1.4
5,869,817	+		PP_5144	0.43	-1.1
5,869,818	-		PP_5144	0.44	+0.7
5,869,863	-		PP_5144	0.50	+1.5
5,869,936	+		PP_5144	0.59	+0.3
5,869,936	+		PP_5144	0.59	+0.8
5,870,123	+		PP_5144	0.84	+0.7
5,870,241	+				-1.0
5,870,288	-				+0.2
5,870,288	-				+1.1
5,870,288	-				+1.0
5,870,319	+				-0.5
5,870,319	+				-0.3
5,870,319	+				+0.7
5,870,319	+				-0.6
5,870,320	-				-0.0
5,870,320	-				+0.7
5,870,320	-				+0.3
5,870,326	+				-0.7
5,870,327	-				-0.6