Strain Fitness in Pseudomonas putida KT2440 around PP_5006

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
5,702,889	+		PP_5005	0.43	+0.3
5,702,889	+		PP_5005	0.43	+1.1
5,702,889	+		PP_5005	0.43	-0.6
5,702,889	+		PP_5005	0.43	-0.0
5,702,890	-		PP_5005	0.43	-0.0
5,702,890	-		PP_5005	0.43	+0.1
5,702,890	-		PP_5005	0.43	+1.0
5,702,890	-		PP_5005	0.43	+0.2
5,702,890	-		PP_5005	0.43	+1.1
5,702,890	-		PP_5005	0.43	+0.3
5,702,973	+		PP_5005	0.48	+0.6
5,702,973	+		PP_5005	0.48	+0.4
5,702,973	+		PP_5005	0.48	+0.4
5,702,974	-		PP_5005	0.48	+1.5
5,703,015	-		PP_5005	0.50	+1.1
5,703,132	+		PP_5005	0.57	+0.3
5,703,132	+		PP_5005	0.57	+0.7
5,703,300	+		PP_5005	0.67	+0.2
5,703,435	+		PP_5005	0.75	+2.7
5,703,436	-		PP_5005	0.75	+1.7
5,703,546	+		PP_5005	0.82	-0.5
5,703,645	+		PP_5005	0.88	+0.8
5,703,645	+		PP_5005	0.88	-0.1
5,703,648	+		PP_5005	0.88	+0.2
5,703,648	+		PP_5005	0.88	+0.1
5,703,648	+		PP_5005	0.88	+0.8
5,703,648	+		PP_5005	0.88	+0.1
5,703,649	-		PP_5005	0.88	+2.1
5,703,649	-		PP_5005	0.88	+1.2
5,703,779	+				+1.1
5,703,779	+				+1.4
5,703,779	+				-0.2
5,703,801	+				+0.7
5,703,801	+				+0.5
5,703,801	+				-0.5
5,703,831	+				-0.0
5,703,831	+				+0.4
5,703,831	+				+0.3
5,703,831	+				+0.3
5,703,831	+				+1.3
5,703,831	+				+0.7
5,703,831	+				+0.7
5,703,831	+				+0.2
5,703,832	-				+0.4
5,703,832	-				+0.8
5,703,832	-				-0.8
5,703,928	+		PP_5006	0.11	+1.1
5,703,929	-		PP_5006	0.11	+0.6
5,703,978	+		PP_5006	0.19	+1.9
5,703,979	-		PP_5006	0.19	+0.5
5,703,981	+		PP_5006	0.20	+0.5
5,703,981	+		PP_5006	0.20	+2.7
5,703,982	-		PP_5006	0.20	+1.2
5,703,982	-		PP_5006	0.20	+0.9
5,704,135	-		PP_5006	0.45	-0.1
5,704,271	-		PP_5006	0.67	+1.0
5,704,280	+		PP_5006	0.68	+0.9
5,704,281	-		PP_5006	0.68	+0.4
5,704,386	+		PP_5006	0.85	-0.0
5,704,386	+		PP_5006	0.85	+0.7
5,704,386	+		PP_5006	0.85	+1.4
5,704,490	+				+0.1
5,704,490	+				-1.7
5,704,490	+				-1.3
5,704,490	+				+1.0
5,704,490	+				-0.4
5,704,491	-				-0.6
5,704,491	-				+1.1
5,704,491	-				+2.7
5,704,529	+				+1.1
5,704,529	+				+2.7
5,704,550	+				+0.3
5,704,551	-				-0.5
5,704,884	-		PP_5007	0.38	+1.1
5,705,275	+		PP_5007	0.88	-0.3
5,705,279	-		PP_5007	0.88	+2.7
5,705,280	-		PP_5007	0.88	+2.7
5,705,280	-		PP_5007	0.88	-2.0
5,705,384	+				+0.9
5,705,470	-		PP_5008	0.21	-0.9