Strain Fitness in Pseudomonas putida KT2440 around PP_4964

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4963 and PP_4964 are separated by 12 nucleotidesPP_4964 and PP_4965 are separated by 151 nucleotides PP_4963: PP_4963 - Phosphoglycerate kinase, at 5,653,311 to 5,654,474 _4963 PP_4964: PP_4964 - D-erythrose 4-phosphate dehydrogenase, at 5,654,487 to 5,655,545 _4964 PP_4965: PP_4965 - transketolase A, at 5,655,697 to 5,657,694 _4965 Position (kb) 5654 5655 5656Strain fitness (log2 ratio) -1 0 1 2 3 4at 5654.818 kb on - strand, within PP_4964at 5654.824 kb on - strand, within PP_4964at 5654.951 kb on - strand, within PP_4964at 5655.007 kb on + strand, within PP_4964at 5655.007 kb on + strand, within PP_4964at 5655.007 kb on + strand, within PP_4964at 5655.008 kb on - strand, within PP_4964at 5655.008 kb on - strand, within PP_4964at 5655.227 kb on + strand, within PP_4964at 5655.228 kb on - strand, within PP_4964at 5655.229 kb on + strand, within PP_4964at 5655.229 kb on + strand, within PP_4964at 5655.229 kb on + strand, within PP_4964at 5655.230 kb on - strand, within PP_4964at 5655.306 kb on + strand, within PP_4964

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
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5,654,818 - PP_4964 0.31 +1.7
5,654,824 - PP_4964 0.32 -0.1
5,654,951 - PP_4964 0.44 +2.7
5,655,007 + PP_4964 0.49 +2.7
5,655,007 + PP_4964 0.49 +2.7
5,655,007 + PP_4964 0.49 +2.7
5,655,008 - PP_4964 0.49 +2.7
5,655,008 - PP_4964 0.49 +4.7
5,655,227 + PP_4964 0.70 +2.7
5,655,228 - PP_4964 0.70 +2.7
5,655,229 + PP_4964 0.70 +2.7
5,655,229 + PP_4964 0.70 -0.1
5,655,229 + PP_4964 0.70 +2.7
5,655,230 - PP_4964 0.70 +2.7
5,655,306 + PP_4964 0.77 +2.7

Or see this region's nucleotide sequence