Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4636 and PP_4637 are separated by 138 nucleotides PP_4637 and PP_4638 are separated by 227 nucleotides
PP_4636: PP_4636 - acetyl-CoA acetyltransferase, at 5,259,459 to 5,260,637
_4636
PP_4637: PP_4637 - 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase family protein, at 5,260,776 to 5,261,600
_4637
PP_4638: PP_4638 - Methylenetetrahydrofolate reductase, at 5,261,828 to 5,263,315
_4638
Position (kb)
5260
5261
5262 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5259.826 kb on - strand, within PP_4636 at 5259.826 kb on - strand, within PP_4636 at 5259.921 kb on + strand, within PP_4636 at 5259.921 kb on + strand, within PP_4636 at 5259.921 kb on + strand, within PP_4636 at 5259.922 kb on - strand, within PP_4636 at 5259.922 kb on - strand, within PP_4636 at 5259.961 kb on - strand, within PP_4636 at 5259.962 kb on - strand, within PP_4636 at 5260.682 kb on - strand at 5260.731 kb on - strand at 5260.992 kb on + strand, within PP_4637 at 5260.993 kb on - strand, within PP_4637 at 5260.993 kb on - strand, within PP_4637 at 5260.993 kb on - strand, within PP_4637 at 5261.030 kb on - strand, within PP_4637 at 5261.119 kb on + strand, within PP_4637 at 5261.165 kb on - strand, within PP_4637 at 5261.176 kb on + strand, within PP_4637 at 5261.176 kb on + strand, within PP_4637 at 5261.278 kb on + strand, within PP_4637 at 5261.278 kb on + strand, within PP_4637 at 5261.278 kb on + strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.279 kb on - strand, within PP_4637 at 5261.350 kb on + strand, within PP_4637 at 5261.350 kb on + strand, within PP_4637 at 5261.351 kb on - strand, within PP_4637 at 5261.351 kb on - strand, within PP_4637 at 5261.351 kb on - strand, within PP_4637 at 5261.462 kb on + strand, within PP_4637 at 5261.462 kb on + strand, within PP_4637 at 5261.463 kb on - strand, within PP_4637 at 5261.585 kb on + strand at 5261.585 kb on + strand at 5261.586 kb on - strand at 5261.586 kb on - strand at 5261.586 kb on - strand at 5261.586 kb on - strand at 5261.827 kb on + strand at 5261.828 kb on - strand at 5261.875 kb on - strand at 5261.875 kb on - strand at 5262.138 kb on + strand, within PP_4638 at 5262.139 kb on - strand, within PP_4638 at 5262.305 kb on + strand, within PP_4638 at 5262.305 kb on + strand, within PP_4638 at 5262.305 kb on + strand, within PP_4638 at 5262.394 kb on - strand, within PP_4638 at 5262.394 kb on - strand, within PP_4638 at 5262.592 kb on + strand, within PP_4638 at 5262.598 kb on + strand, within PP_4638 at 5262.599 kb on - strand, within PP_4638
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 5,259,826 - PP_4636 0.31 -0.9 5,259,826 - PP_4636 0.31 -0.3 5,259,921 + PP_4636 0.39 -0.5 5,259,921 + PP_4636 0.39 +0.2 5,259,921 + PP_4636 0.39 +0.1 5,259,922 - PP_4636 0.39 +0.5 5,259,922 - PP_4636 0.39 -0.0 5,259,961 - PP_4636 0.43 +0.5 5,259,962 - PP_4636 0.43 -1.1 5,260,682 - +0.7 5,260,731 - +0.4 5,260,992 + PP_4637 0.26 +0.7 5,260,993 - PP_4637 0.26 +1.2 5,260,993 - PP_4637 0.26 -0.8 5,260,993 - PP_4637 0.26 +0.5 5,261,030 - PP_4637 0.31 -2.9 5,261,119 + PP_4637 0.42 +0.9 5,261,165 - PP_4637 0.47 +0.2 5,261,176 + PP_4637 0.48 +0.7 5,261,176 + PP_4637 0.48 -0.2 5,261,278 + PP_4637 0.61 +0.9 5,261,278 + PP_4637 0.61 +1.6 5,261,278 + PP_4637 0.61 -0.8 5,261,279 - PP_4637 0.61 +1.3 5,261,279 - PP_4637 0.61 +0.1 5,261,279 - PP_4637 0.61 -0.3 5,261,279 - PP_4637 0.61 -1.9 5,261,279 - PP_4637 0.61 +0.2 5,261,350 + PP_4637 0.70 -0.5 5,261,350 + PP_4637 0.70 +0.2 5,261,351 - PP_4637 0.70 +0.3 5,261,351 - PP_4637 0.70 -1.0 5,261,351 - PP_4637 0.70 +1.7 5,261,462 + PP_4637 0.83 +1.2 5,261,462 + PP_4637 0.83 +1.0 5,261,463 - PP_4637 0.83 +1.1 5,261,585 + -0.9 5,261,585 + +1.0 5,261,586 - -0.8 5,261,586 - -0.2 5,261,586 - -0.1 5,261,586 - -0.1 5,261,827 + +0.6 5,261,828 - -0.1 5,261,875 - +1.3 5,261,875 - +0.3 5,262,138 + PP_4638 0.21 -0.6 5,262,139 - PP_4638 0.21 +0.1 5,262,305 + PP_4638 0.32 +0.6 5,262,305 + PP_4638 0.32 -1.2 5,262,305 + PP_4638 0.32 -1.1 5,262,394 - PP_4638 0.38 +0.1 5,262,394 - PP_4638 0.38 -0.5 5,262,592 + PP_4638 0.51 -0.6 5,262,598 + PP_4638 0.52 -1.0 5,262,599 - PP_4638 0.52 -1.0
Or see this region's nucleotide sequence