Strain Fitness in Pseudomonas putida KT2440 around PP_4448

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
5,041,976	-		PP_4447	0.23	-0.2
5,042,016	-		PP_4447	0.27	+0.3
5,042,141	+		PP_4447	0.37	+0.8
5,042,148	-		PP_4447	0.37	-1.9
5,042,148	-		PP_4447	0.37	+0.3
5,042,217	+		PP_4447	0.43	-0.2
5,042,218	-		PP_4447	0.43	-1.5
5,042,294	+		PP_4447	0.49	-1.3
5,042,295	-		PP_4447	0.49	+0.2
5,042,351	+		PP_4447	0.53	+0.8
5,042,717	+		PP_4447	0.83	-0.2
5,042,720	+		PP_4447	0.83	-0.4
5,042,972	+				+0.6
5,043,001	+				-1.6
5,043,056	-				+0.3
5,043,176	+		PP_4448	0.14	+0.8
5,043,236	+		PP_4448	0.17	+0.2
5,043,278	+		PP_4448	0.20	-0.6
5,043,362	+		PP_4448	0.24	-1.1
5,043,362	+		PP_4448	0.24	-0.5
5,043,564	-		PP_4448	0.36	-0.2
5,043,639	+		PP_4448	0.40	-0.4
5,043,643	+		PP_4448	0.40	-0.6
5,043,804	-		PP_4448	0.49	-1.2
5,043,815	-		PP_4448	0.50	-0.9
5,043,850	+		PP_4448	0.52	-0.3
5,043,875	-		PP_4448	0.54	-0.4
5,044,074	+		PP_4448	0.65	-0.4
5,044,151	-		PP_4448	0.69	-1.1
5,044,185	+		PP_4448	0.71	-0.3
5,044,191	+		PP_4448	0.71	-1.1
5,044,249	+		PP_4448	0.75	-1.8
5,044,249	+		PP_4448	0.75	-1.2
5,044,273	-		PP_4448	0.76	-1.5
5,044,273	-		PP_4448	0.76	+0.5
5,044,464	+		PP_4448	0.87	-2.1
5,044,464	+		PP_4448	0.87	-0.8
5,044,465	-		PP_4448	0.87	-0.6
5,044,688	+				-0.1
5,044,688	+				+0.5
5,044,689	-				-0.2
5,044,689	-				-0.5
5,044,692	+				-0.6
5,044,692	+				-0.0
5,044,692	+				+0.5
5,044,693	-				+1.0
5,044,743	-				+0.4
5,044,743	-				+0.4
5,044,743	-				-0.4
5,044,743	-				-0.9
5,044,808	+				+1.8
5,044,809	-				+1.0
5,044,819	+				+0.1
5,044,819	+				-0.1
5,044,819	+				-0.2
5,044,866	+				-1.1
5,044,867	-				-0.8
5,044,930	-				-0.0
5,044,934	+				+0.0
5,044,934	+				+1.0
5,044,934	+				+0.5
5,044,934	+				-0.5
5,044,935	-				-1.6
5,045,042	+		PP_5681	0.34	-0.2
5,045,043	-		PP_5681	0.34	+1.2
5,045,044	+		PP_5681	0.35	+0.3
5,045,045	-		PP_5681	0.35	+0.5
5,045,045	-		PP_5681	0.35	-0.9
5,045,045	-		PP_5681	0.35	+0.1
5,045,045	-		PP_5681	0.35	+0.4
5,045,130	-		PP_5681	0.71	+1.2
5,045,259	+				+0.1
5,045,293	+				+0.6
5,045,298	+				-0.4
5,045,312	-				+0.1
5,045,312	-				-0.0
5,045,325	-				+0.4
5,045,329	+				+0.5
5,045,330	-				-2.7
5,045,381	-				+0.2
5,045,393	+				-0.3
5,045,393	+				+0.1
5,045,393	+				+0.8
5,045,475	+		PP_4449	0.38	-0.6
5,045,476	-		PP_4449	0.38	-0.3
5,045,530	+		PP_4449	0.59	-0.7
5,045,531	-		PP_4449	0.60	-0.3
5,045,570	+		PP_4449	0.75	+0.8
5,045,570	+		PP_4449	0.75	-0.1
5,045,625	+				-0.7
5,045,625	+				-0.4
5,045,626	-				-0.8
5,045,626	-				+1.4