Strain Fitness in Pseudomonas putida KT2440 around PP_4221

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,786,730	+				+0.9
4,786,731	-				+1.9
4,786,970	+				+0.8
4,786,971	-				+0.2
4,786,971	-				+1.2
4,787,092	+				+0.8
4,787,092	+				+1.1
4,787,097	+				+0.1
4,787,098	-				+1.3
4,787,099	+				+0.9
4,787,153	+				-0.3
4,787,153	+				+0.2
4,787,153	+				-0.2
4,787,154	-				+0.7
4,787,154	-				-0.6
4,787,331	+				+0.9
4,787,449	+				-0.2
4,787,450	-				+0.6
4,787,473	+				-0.1
4,787,474	-				+0.7
4,787,594	-				+1.1
4,787,613	+				-0.4
4,787,702	+				+0.5
4,787,703	-				-0.4
4,787,703	-				-0.1
4,787,724	+				-0.8
4,787,724	+				-0.3
4,787,724	+				-0.0
4,787,724	+				-0.9
4,787,725	-				-0.2
4,787,882	+				+0.3
4,787,883	-				+0.6
4,787,974	-		PP_4221	0.10	+1.0
4,788,245	+		PP_4221	0.14	-0.4
4,788,245	+		PP_4221	0.14	-1.6
4,788,246	-		PP_4221	0.14	+0.3
4,788,246	-		PP_4221	0.14	+2.9
4,788,246	-		PP_4221	0.14	+0.4
4,788,246	-		PP_4221	0.14	+0.2
4,788,246	-		PP_4221	0.14	-0.1
4,788,246	-		PP_4221	0.14	+1.2
4,788,249	+		PP_4221	0.14	+0.7
4,788,249	+		PP_4221	0.14	+0.5
4,788,249	+		PP_4221	0.14	+0.6
4,788,250	-		PP_4221	0.14	+0.2
4,788,300	+		PP_4221	0.15	+1.9
4,788,300	+		PP_4221	0.15	+0.2
4,788,317	+		PP_4221	0.15	+0.1
4,788,317	+		PP_4221	0.15	-1.5
4,788,317	+		PP_4221	0.15	+0.9
4,788,317	+		PP_4221	0.15	-0.2
4,788,469	-		PP_4221	0.18	+0.9
4,788,470	+		PP_4221	0.18	-0.4
4,788,470	+		PP_4221	0.18	+0.3
4,788,470	+		PP_4221	0.18	-0.6
4,788,470	+		PP_4221	0.18	+1.3
4,788,470	+		PP_4221	0.18	+0.1
4,788,471	-		PP_4221	0.18	+1.0
4,788,732	+		PP_4221	0.22	-0.2
4,788,732	+		PP_4221	0.22	+0.9
4,788,733	-		PP_4221	0.22	-0.4
4,788,733	-		PP_4221	0.22	-1.6
4,788,733	-		PP_4221	0.22	+0.3
4,788,741	+		PP_4221	0.22	+1.1
4,788,741	+		PP_4221	0.22	-0.1
4,788,742	-		PP_4221	0.22	+0.4
4,788,742	-		PP_4221	0.22	+1.2
4,788,903	+		PP_4221	0.24	+1.1
4,788,903	+		PP_4221	0.24	+0.7
4,788,945	+		PP_4221	0.25	+0.2
4,788,945	+		PP_4221	0.25	-1.0
4,788,946	-		PP_4221	0.25	-1.0
4,788,946	-		PP_4221	0.25	-1.0
4,788,946	-		PP_4221	0.25	+1.4
4,789,092	+		PP_4221	0.27	+0.5
4,789,093	-		PP_4221	0.27	+2.9
4,789,328	+		PP_4221	0.31	+0.9
4,789,328	+		PP_4221	0.31	-0.6
4,789,329	-		PP_4221	0.31	+0.4
4,789,329	-		PP_4221	0.31	+1.1
4,789,329	-		PP_4221	0.31	+1.4
4,789,359	+		PP_4221	0.31	-0.0
4,789,359	+		PP_4221	0.31	-0.4
4,789,360	-		PP_4221	0.31	+0.4
4,789,512	+				+2.9
4,789,512	+		PP_4221	0.34	-0.8
4,789,512	+		PP_4221	0.34	+0.8
4,789,513	-		PP_4221	0.34	+0.9
4,789,513	-		PP_4221	0.34	-0.2
4,789,513	-		PP_4221	0.34	-0.7
4,789,536	+		PP_4221	0.34	+0.5
4,789,536	+		PP_4221	0.34	+0.5
4,789,537	-		PP_4221	0.34	+1.3
4,789,628	-		PP_4221	0.35	-0.1
4,789,660	-		PP_4221	0.36	-0.8
4,789,806	-		PP_4221	0.38	+1.1
4,789,940	+		PP_4221	0.40	+0.2
4,789,940	+		PP_4221	0.40	+0.8
4,789,984	-		PP_4221	0.41	+1.3
4,790,040	+		PP_4221	0.42	-0.7
4,790,049	+		PP_4221	0.42	-0.4
4,790,050	-		PP_4221	0.42	-0.6
4,790,050	-		PP_4221	0.42	+1.4
4,790,050	-		PP_4221	0.42	+1.3
4,790,106	+		PP_4221	0.43	+0.6
4,790,106	+		PP_4221	0.43	+0.2
4,790,183	+		PP_4221	0.44	+2.9
4,790,183	+		PP_4221	0.44	+1.0
4,790,183	+		PP_4221	0.44	+2.0
4,790,209	-		PP_4221	0.44	+2.3
4,790,209	-		PP_4221	0.44	+1.2
4,790,386	+		PP_4221	0.47	-0.4
4,790,387	-		PP_4221	0.47	+0.2
4,790,530	-		PP_4221	0.49	+0.3
4,791,149	+		PP_4221	0.59	-0.3
4,791,149	+		PP_4221	0.59	+1.2
4,791,180	+		PP_4221	0.59	+0.9
4,791,422	+		PP_4221	0.63	-1.4
4,791,458	+		PP_4221	0.63	+0.4
4,791,458	+		PP_4221	0.63	-0.1
4,791,716	+		PP_4221	0.67	+0.4
4,791,716	+		PP_4221	0.67	+1.3
4,791,717	-		PP_4221	0.67	-0.4
4,791,717	-		PP_4221	0.67	-0.4
4,791,754	+		PP_4221	0.68	+1.3
4,791,754	+		PP_4221	0.68	+0.3
4,791,754	+		PP_4221	0.68	+0.2
4,791,755	-		PP_4221	0.68	+0.4
4,791,883	+		PP_4221	0.70	-0.9
4,791,904	-		PP_4221	0.70	-0.6
4,791,913	-		PP_4221	0.70	+1.1
4,792,097	+		PP_4221	0.73	+0.4
4,792,097	+		PP_4221	0.73	+1.7
4,792,098	-		PP_4221	0.73	+1.6
4,792,101	+		PP_4221	0.73	-0.5
4,792,101	+		PP_4221	0.73	+1.6
4,792,101	+		PP_4221	0.73	+1.3
4,792,101	+		PP_4221	0.73	+0.8
4,792,101	+		PP_4221	0.73	+0.1
4,792,102	-		PP_4221	0.73	+1.9
4,792,102	-		PP_4221	0.73	+0.3
4,792,102	-		PP_4221	0.73	-0.7
4,792,102	-		PP_4221	0.73	+0.5
4,792,248	+		PP_4221	0.76	-0.1
4,792,540	+		PP_4221	0.80	+0.3
4,792,820	-		PP_4221	0.84	+0.9
4,792,908	+		PP_4221	0.86	-1.1
4,792,908	+		PP_4221	0.86	+0.9
4,792,908	+		PP_4221	0.86	+2.3
4,792,909	-		PP_4221	0.86	+0.0
4,792,909	-		PP_4221	0.86	+0.9
4,792,914	+		PP_4221	0.86	-0.3
4,792,915	-		PP_4221	0.86	-0.2
4,792,915	-		PP_4221	0.86	-0.3
4,793,115	+		PP_4221	0.89	-1.3
4,793,235	+				+0.8
4,793,264	-				+1.3
4,793,290	+				+0.4
4,793,290	+				-0.6
4,793,291	-				-0.7
4,793,291	-				+0.9
4,793,291	-				-0.9
4,793,625	+				+0.3
4,793,626	-				+1.6
4,793,626	-				+0.7
4,793,679	+				+0.8
4,793,679	+				+0.2
4,793,679	+				+0.4
4,793,680	-				+1.5
4,793,852	+				-0.4
4,793,853	-				+1.5
4,794,200	+		PP_4222	0.21	+0.1
4,794,201	-		PP_4222	0.21	+0.1
4,794,201	-		PP_4222	0.21	+1.8
4,794,201	-		PP_4222	0.21	-0.4
4,794,266	-		PP_4222	0.28	+0.8
4,794,305	-		PP_4222	0.32	+1.9
4,794,305	-		PP_4222	0.32	+0.5
4,794,305	-		PP_4222	0.32	+0.7
4,794,410	+		PP_4222	0.42	+0.2
4,794,410	+		PP_4222	0.42	+0.3
4,794,410	+		PP_4222	0.42	+0.1
4,794,422	-		PP_4222	0.43	+0.3
4,794,479	+				+0.3
4,794,480	-		PP_4222	0.49	-0.0
4,794,491	+		PP_4222	0.50	+0.1
4,794,491	+		PP_4222	0.50	+1.1
4,794,492	-		PP_4222	0.51	+0.8
4,794,523	+		PP_4222	0.54	+0.4
4,794,523	+		PP_4222	0.54	-0.4
4,794,606	-		PP_4222	0.62	+0.0
4,794,606	-		PP_4222	0.62	+0.1
4,794,606	-		PP_4222	0.62	+0.5
4,794,606	-		PP_4222	0.62	-0.2
4,794,638	+		PP_4222	0.65	+0.9
4,794,638	+		PP_4222	0.65	+0.1
4,794,681	-		PP_4222	0.70	+1.7
4,794,695	+		PP_4222	0.71	-0.4
4,794,695	+		PP_4222	0.71	+0.2
4,794,696	-		PP_4222	0.71	-0.2
4,794,696	-		PP_4222	0.71	+0.3
4,794,740	+		PP_4222	0.76	+0.6