Strain Fitness in Pseudomonas putida KT2440 around PP_4216

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,762,657	+				+0.5
4,762,945	+		PP_4215	0.31	-0.8
4,762,975	+		PP_4215	0.34	+2.9
4,762,975	+		PP_4215	0.34	-1.5
4,762,975	+		PP_4215	0.34	-0.7
4,762,975	+		PP_4215	0.34	+0.9
4,762,975	+		PP_4215	0.34	-1.9
4,763,053	+		PP_4215	0.43	-0.9
4,763,053	+		PP_4215	0.43	+0.2
4,763,053	+		PP_4215	0.43	+1.3
4,763,053	+		PP_4215	0.43	-0.1
4,763,110	+		PP_4215	0.49	+0.1
4,763,110	+		PP_4215	0.49	+0.1
4,763,338	+		PP_4215	0.75	-0.3
4,763,338	+		PP_4215	0.75	+0.9
4,763,339	-		PP_4215	0.75	+0.5
4,763,359	+		PP_4215	0.77	+0.1
4,763,359	+		PP_4215	0.77	-0.7
4,763,359	+		PP_4215	0.77	+0.6
4,764,770	+		PP_4216	0.70	+0.5
4,764,771	-				+0.3
4,764,869	+		PP_4216	0.76	+2.9
4,765,181	+				-0.4
4,765,648	+		PP_4217	0.12	+0.4
4,765,648	+		PP_4217	0.12	-1.7
4,765,648	+		PP_4217	0.12	-0.9
4,765,649	-		PP_4217	0.12	-2.5
4,765,731	+		PP_4217	0.16	-1.1
4,765,732	-		PP_4217	0.16	-2.8
4,765,768	+		PP_4217	0.17	-2.2
4,765,768	+		PP_4217	0.17	-3.0
4,765,768	+		PP_4217	0.17	-3.1
4,765,770	+		PP_4217	0.17	-2.5
4,766,136	+		PP_4217	0.32	-2.8
4,766,189	-		PP_4217	0.34	-1.1
4,766,261	-		PP_4217	0.37	-0.9
4,766,261	-		PP_4217	0.37	-3.4
4,766,261	-		PP_4217	0.37	-0.4