Strain Fitness in Pseudomonas putida KT2440 around PP_4211

Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
4,755,880	-		PP_4210	0.57	-0.9
4,756,044	+		PP_4210	0.65	+0.9
4,756,045	-		PP_4210	0.65	-0.2
4,756,048	+		PP_4210	0.66	+0.4
4,756,048	+		PP_4210	0.66	-1.9
4,756,048	+		PP_4210	0.66	+0.1
4,756,049	-		PP_4210	0.66	-0.1
4,756,049	-		PP_4210	0.66	+0.7
4,756,049	-		PP_4210	0.66	-1.2
4,756,049	-		PP_4210	0.66	+0.3
4,756,063	+		PP_4210	0.66	+0.2
4,756,063	+		PP_4210	0.66	-0.7
4,756,063	+		PP_4210	0.66	-1.1
4,756,064	-		PP_4210	0.66	-0.6
4,756,193	+		PP_4210	0.73	-1.8
4,756,194	-		PP_4210	0.73	+1.4
4,756,408	-		PP_4210	0.84	-1.8
4,756,577	+				-0.2
4,756,578	-				+0.2
4,756,712	+				+0.1
4,756,712	+				-0.5
4,756,805	+				-1.2
4,756,805	+				-0.0
4,756,805	+				-1.2
4,756,813	+				+1.5
4,756,994	-		PP_4211	0.19	+0.5
4,756,994	-		PP_4211	0.19	+0.2
4,757,265	+		PP_4211	0.38	+0.4
4,757,266	-		PP_4211	0.38	-0.1
4,757,300	+		PP_4211	0.40	+0.1
4,757,303	-		PP_4211	0.41	-0.1
4,757,734	-		PP_4211	0.71	+0.2
4,757,734	-		PP_4211	0.71	+0.9
4,757,736	+		PP_4211	0.71	-0.3
4,757,737	-		PP_4211	0.71	+0.2
4,757,737	-		PP_4211	0.71	-0.3
4,757,872	+		PP_4211	0.81	-0.6
4,757,873	-		PP_4211	0.81	+0.4
4,758,031	-				-0.1
4,758,151	+				+0.2
4,758,152	-				+0.1
4,758,195	+				+0.9
4,758,196	-				-0.2
4,758,221	+				-0.8
4,758,252	+				-1.0
4,758,328	+		PP_4212	0.10	+0.1
4,758,561	+		PP_4212	0.25	-1.1
4,758,562	-		PP_4212	0.25	-1.3
4,758,718	-				+3.4
4,758,804	+		PP_4212	0.40	-0.2
4,758,805	-		PP_4212	0.40	-0.8
4,758,820	-		PP_4212	0.41	-0.6
4,758,867	+		PP_4212	0.43	+0.6