Experiment: 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5655 and PP_4094 are separated by 12 nucleotides
PP_5655: PP_5655 - protein of unknown function, at 4,629,582 to 4,629,728
_5655
PP_4094: PP_4094 - conserved protein of unknown function, at 4,629,741 to 4,630,226
_4094
Position (kb)
4629
4630
4631 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4628.836 kb on - strand at 4628.958 kb on - strand at 4629.050 kb on + strand at 4629.125 kb on - strand at 4629.154 kb on + strand at 4629.154 kb on + strand at 4629.249 kb on - strand at 4629.290 kb on + strand at 4629.312 kb on + strand at 4629.313 kb on - strand at 4629.313 kb on - strand at 4629.338 kb on + strand at 4629.353 kb on + strand at 4629.353 kb on + strand at 4629.421 kb on - strand at 4629.421 kb on - strand at 4629.421 kb on - strand at 4629.422 kb on + strand at 4629.513 kb on + strand at 4629.520 kb on + strand at 4629.526 kb on + strand at 4629.737 kb on - strand at 4629.752 kb on - strand at 4629.761 kb on - strand at 4629.823 kb on + strand, within PP_4094 at 4629.840 kb on - strand, within PP_4094 at 4629.844 kb on - strand, within PP_4094 at 4630.219 kb on - strand at 4630.332 kb on + strand at 4630.458 kb on + strand at 4630.461 kb on + strand at 4630.566 kb on - strand at 4630.596 kb on + strand at 4630.596 kb on + strand at 4630.596 kb on + strand at 4630.596 kb on + strand at 4630.597 kb on - strand at 4630.647 kb on - strand at 4631.225 kb on + strand at 4631.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 1g/hr dextrose, set to 30% DO. remove 4,628,836 - -0.3 4,628,958 - +0.5 4,629,050 + +0.2 4,629,125 - +2.8 4,629,154 + +0.1 4,629,154 + -0.3 4,629,249 - -1.9 4,629,290 + -0.6 4,629,312 + +0.2 4,629,313 - -0.3 4,629,313 - +1.3 4,629,338 + +0.1 4,629,353 + -0.1 4,629,353 + -0.5 4,629,421 - +1.8 4,629,421 - -0.2 4,629,421 - -0.1 4,629,422 + +0.8 4,629,513 + -0.4 4,629,520 + -0.5 4,629,526 + -1.5 4,629,737 - +0.4 4,629,752 - +0.9 4,629,761 - -0.2 4,629,823 + PP_4094 0.17 -0.4 4,629,840 - PP_4094 0.20 -1.4 4,629,844 - PP_4094 0.21 -0.9 4,630,219 - +0.4 4,630,332 + +0.1 4,630,458 + -0.2 4,630,461 + +0.1 4,630,566 - +0.4 4,630,596 + +1.5 4,630,596 + +1.7 4,630,596 + +0.6 4,630,596 + -0.0 4,630,597 - +0.2 4,630,647 - +0.2 4,631,225 + -0.6 4,631,226 - -1.2
Or see this region's nucleotide sequence